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Protein adsorption properties of OEG monolayers and dense PNIPAM brushes probed by neutron reflectivity

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Abstract

The structure of dense poly(N-isopropylacrylamide) (PNIPAM) brushes and oligo(ethylene glycol) (OEG) monolayers has been probed using neutron reflectometry and ellipsometry. The PNIPAM brush is swollen below the Lower Critical Solution Temperature (LCST) of 32 ∘C and is collapsed at 37 ∘C. Neutron reflectivity shows that below the LCST, the brush is described by a two-layer model: an inner dense layer and a hydrated outer layer. Above the LCST the collapsed brush forms a homogenous layer. With a fully deuterated myoglobin protein to increase the neutron scattering length density contrast, the reflectivity data show no detectable primary adsorption on the grafted OEG surface. A bound on the ternary adsorption onto PNIPAM chains forming dense brushes below and above the LCST is obtained.

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Authors and Affiliations

  1. Département de Physique, Faculté des Sciences, Université Libre de Bruxelles, Boulevard du Triomphe, CP. 223, 1050, Bruxelles, Belgique

    N. Brouette & M. Sferrazza

  2. Department of Chemical and Biomolecular Engineering, University of Illinois at Urbana-Champaign, Urbana, Illinois 61801, USA

    C. Xue & D.E. Leckband

  3. Institut Max Von Laue-Paul Langevin, 38042, Grenoble, France

    M. Haertlein, M. Moulin & G. Fragneto

  4. Department of Chemistry, University of Illinois at Urbana-Champaign, Urbana, Illinois 61801, USA

    D.E. Leckband

  5. University of Grenoble 1/CNRS, LIPhy UMR 5588, BP. 87, 38041, Grenoble, France

    A. Halperin

Authors
  1. N. Brouette
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  2. C. Xue
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  3. M. Haertlein
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  4. M. Moulin
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  5. G. Fragneto
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  6. D.E. Leckband
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  7. A. Halperin
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  8. M. Sferrazza
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Correspondence to M. Sferrazza.

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Brouette, N., Xue, C., Haertlein, M. et al. Protein adsorption properties of OEG monolayers and dense PNIPAM brushes probed by neutron reflectivity. Eur. Phys. J. Spec. Top. 213, 343–353 (2012). https://doi.org/10.1140/epjst/e2012-01681-4

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  • DOI: https://doi.org/10.1140/epjst/e2012-01681-4

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