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Diffusion inside living human cells

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Abstract

Naturally occurring lipid granules diffuse in the cytoplasm and can be used as tracers to map out the viscoelastic landscape inside living cells. Using optical trapping and single particle tracking we found that lipid granules exhibit anomalous diffusion inside human umbilical vein endothelial cells. For these cells the exact diffusional pattern of a particular granule depends on the physiological state of the cell and on the localization of the granule within the cytoplasm. Granules located close to the actin rich periphery of the cell move less than those located towards to the center of the cell or within the nucleus. Also, granules in cells which are stressed by intense laser illumination or which have attached to a surface for a long period of time move in a more restricted fashion than those within healthy cells. For granules diffusing in healthy cells, in regions away from the cell periphery, occurrences of weak ergodicity breaking are observed, similar to the recent observations inside living fission yeast cells [1].

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References

  1. J.H. Jeon, V. Tejedor, S. Burov, E. Barkai, C. Selhuber-Unkel, K. Berg-Sørensen, L.B. Oddershede, R. Metzler, Phys. Rev. Lett. 106, 048103 (2011)

    Article  ADS  Google Scholar 

  2. L. Rajendran, H.J. Knolker, K. Simons, Nat. Rev. Drug. Discov. 9, 29 (2010)

    Article  Google Scholar 

  3. D. Moradpour, F. Penin, C.M. Rice, Nature Microbiol. 5, 453 (2007)

    Article  Google Scholar 

  4. R. Metzler, J. Klafter, Physics Reports 339, 1 (2000)

    Article  MathSciNet  ADS  MATH  Google Scholar 

  5. R. Metzler, J. Klafter, J. Phys. A: Math. General 37, R161 (2004)

    Article  MathSciNet  ADS  MATH  Google Scholar 

  6. I. Golding, E. Cox, Phys. Rev. Lett. 96, 098102 (2006)

    Article  ADS  Google Scholar 

  7. S.C. Weber, A.J. Spakowitz, J.A. Theriot, Phys. Rev. Lett. 104, 238102 (2010)

    Article  ADS  Google Scholar 

  8. I.M. Tolić-Nørrelykke, E.L. Munteanu, G. Thon, L.B. Oddershede, K. Berg-Sørensen, Phys. Rev. Lett. 93, 078102 (2004)

    Article  ADS  Google Scholar 

  9. C. Selhuber-Unkel, P. Yde, K. Berg-Sørensen, L.B. Oddershede, Phys. Biol. 6, 025015 (2009)

    Article  ADS  Google Scholar 

  10. S. Yamada, D. Wirtz, S.C. Kuo, Biophys. J. 78, 1736 (2000)

    Article  Google Scholar 

  11. I. Bronstein, Y. Israel, E. Kepten, S. Mai, Y. Shav-Tal, E. Barkai, Y. Garini, Phys. Rev. Lett. 103, 018102 (2009)

    Article  ADS  Google Scholar 

  12. A. Caspi, R. Granek, M. Elbaum, Phys. Rev. E 66, 011916 (2002)

    Article  ADS  Google Scholar 

  13. G. Guigas, M. Weiss, Biophys. J. 94, 90 (2008)

    Article  ADS  Google Scholar 

  14. R.J. Ellis, A.P. Minton, Nature 425, 27 (2003)

    Article  ADS  Google Scholar 

  15. S.R. McGuffee, A.H. Elcock, PLoS Comput Biol. 6, e1000694 (2010)

    Article  Google Scholar 

  16. M.J. Saxton, K. Jacobson, Annual Rev. Biophys. Biomolec. Struct. 26, 373 (1997)

    Article  Google Scholar 

  17. G. Seisenberger, M.U. Ried, T. Endreß, H. Büning, M. Hallek, C. Bräuchle, Science 294, 1929 (2001)

    Article  ADS  Google Scholar 

  18. V. Tejedor, O. Benichou, R. Voituriez, R. Jungmann, F. Simmel, C. Selhuber-Unkel, L. Oddershede, R. Metzler, Biophys. J. 98, 1364 (2010)

    Article  ADS  Google Scholar 

  19. J. Mercer, M. Schelhaas, A. Helenius, Annu. Rev. Biochem. 79, 803 (2010)

    Article  Google Scholar 

  20. D.A. Fletcher, R.D. Mullins, Nature 463, 485 (2010)

    Article  ADS  Google Scholar 

  21. Y. He, S. Burov, R. Metzler, E. Barkai, Phys. Rev. Lett. 101, 058101 (2008)

    Article  ADS  Google Scholar 

  22. S. Burov, R. Metzler, E. Barkai, Proc. National Acad. Sci. 107, 13228 (2010)

    Article  MathSciNet  ADS  MATH  Google Scholar 

  23. S. Burov, J.H. Jeon, R. Metzler, E. Barkai, Phys. Chem. Chem. Phys. 13, 1800 (2011)

    Article  Google Scholar 

  24. A. Lubelski, I.M. Sokolov, J. Klafter, Phys. Rev. Lett. 100, 250602 (2008)

    Article  ADS  Google Scholar 

  25. T. Neusius, I.M. Sokolov, J.C. Smith, Phys. Rev. E 80, 011109 (2009)

    Article  ADS  Google Scholar 

  26. M. Rasmussen, L. Oddershede, H. Siegumfeldt, Appl. Environm. Microbiol. 74, 2441 (2008)

    Article  Google Scholar 

  27. E. Peterman, F. Gittes, C. Schmidt, Biophys. J. 84, 1308 (2003)

    Article  ADS  Google Scholar 

  28. P. Bendix, S. Reihani, L. Oddershede, ACS Nano 4, 2256 (2010)

    Article  Google Scholar 

  29. A.C. Richardson, S.N.S. Reihani, L.B. Oddershede, Opt. Express 16, 15709 (2008)

    Article  ADS  Google Scholar 

  30. S.S. Rogers, T.A. Waigh, X. Zhao, J.R. Lu, Phys. Biol. 4, 220 (2007)

    Article  ADS  Google Scholar 

  31. A.V. Weigel, B. Simon, M.M. Tamkun, D. Krapf, Proc. National Acad. Sci. 108, 6438 (2011)

    Article  ADS  Google Scholar 

  32. N. Rossen, A. Hansen, C. Selhuber-Unkel, L. Oddershede, PLoS ONE 6, e25196 (2011)

    Article  ADS  Google Scholar 

  33. F. Gittes, F.C. Mackintosh, Phys. Rev. E 58, R1241 (1998)

    Article  ADS  Google Scholar 

  34. I.Y. Wong, M.L. Gardel, D.R. Reichman, E.R. Weeks, M.T. Valentine, A.R. Bausch, D.A. Weitz, Phys. Rev. Lett. 92, 178101 (2004)

    Article  ADS  Google Scholar 

  35. J.L. McGrath, Current Biol. 17, R357 (2007)

    Article  Google Scholar 

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Correspondence to L. B. Oddershede.

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Leijnse, N., Jeon, J.H., Loft, S. et al. Diffusion inside living human cells. Eur. Phys. J. Spec. Top. 204, 75–84 (2012). https://doi.org/10.1140/epjst/e2012-01553-y

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