Abstract
We have studied p H-dependent conformational transitions of Bovine Serum Albumin adsorbed onto different sizes of gold nanospheres. For larger spheres (D > 10 nm) there is evidence for a path-dependent extended state near p H 4, over a very small p H range. For smaller nanospheres (5nm and 10nm) the evidence for such a transition is either much weaker or completely suppressed. We suggest that the absence of the transition on small spheres is due to the fact that the protein adsorbed on such small spheres has already lost at least some of its tertiary structure. The results have important implications for the functionality of proteins adsorbed onto nanospheres or surfaces with nm scale roughness.
Similar content being viewed by others
References
W.T. Liu, J. Biosci. Bioeng. 102, 1 (2006).
X. Huang, I.H. El-Sayed, W. Qian, M.A. El-Sayed, J. Am. Chem. Soc. 128, 2115 (2006).
X. Huang, P.K. Jain, I.H. El-Sayed, M.A. El-Sayed, Photochem. Photobiol. 82, 412 (2006).
D.O. Lapotko, E. Lukianova, A.A. Oraevsky, Lasers Surg. Med. 38, 631 (2006).
I. Hilger, R. Hergt, W.A. Kaiser, IEE Proc. Nanobiotechnol. 152, 33 (2005).
M.A. Hayat, Colloidal Gold: Principles, Methods, and Applications (Academic Press, San Diego, Toronto, 1989).
V.P. Zharov, K.E. Mercer, E.N. Galitovskaya, M.S. Smeltzer, Biophys. J. 90, 619 (2006).
C. Haynes, W. Norde, Colloids Surf. B: Biointerfaces 2, 517 (1994).
M. Malmsten, Biopolymers at Interfaces, 2nd edition (M. Dekker, New York, Basel, 2003).
Q. Garrett, B. Laycock, R.W. Garrett, Invest. Opthal. Vis. Sci. 41, 1687 (2000).
R.J. Green, I. Hopkinson, R.A.L. Jones, Langmuir 15, 5102 (1999).
E. Ostuni, B.A. Grzybowski, M. Mrksich, C.S. Roberts, G.M. Whitesides, Langmuir 19, 1861 (2003).
N. Xia, Y. Hu, D.W. Grainger, D.G. Castner, Langmuir 18, 3255 (2002).
A.A. Vertegel, R.W. Siegel, J.S. Dordick, Langmuir 20, 6800 (2004).
M. Lundqvist, I. Sethson, B.-H. Jonsson, Langmuir 20, 10639 (2004).
P. Roach, D. Farrar, C.C. Perry, J. Am. Chem. Soc. 128, 3939 (2006).
J.H. Teichroeb, J.A. Forrest, V. Ngai, L.W. Jones, Eur. Phys. J. E 21, 19 (2006).
B. Dalmas, W.H. Bannister, Anal Biochem. 225, 39 (1995).
L. Shang, Y. Wang, J. Jiang, S. Dong, Langmuir 23, 2714 (2007).
P. Englebienne, A. Van oonacker, M. Verhas, Spectroscopy 17, 255 (2003).
N.G. Khlebtsov, V.A. Bogatyrev, L.A. Dykman, A.G. Melnikov, J. Colloid Interface Sci. 180, 436 (1996).
S. Chah, M.R. Hammond, R.N. Zare, Chem. & Biol. 12, 323 (2005).
D.C. Carter, J.X. Ho, Adv. Protein Chem. 45, 153 (1994).
M.Y. Khan, Biochem. J. 236, 307 (1986).
W.F. Harrington, P. Johnson, R.H. Ottewill, Biochem. J. 62, 569 (1956).
M.Y. Khan, S.K. Agarwal, S. Hangloo, J. Biochem. 102, 313 (1987).
C.F. Bohren, D.R. Huffman, Absorption and Scattering of Light by Small Particles (Wiley, New York, Toronto, 1983).
C. Matzler, Research Report No. 2002-08, Institute of Applied Physics, University of Bern, Switzerland (2002).
H. Arwin, Appl. Spectrosc. 40, 313 (1986).
T.J. Su, J.R. Lu, R.K. Thomas, Z.F. Cui, J. Penfold, J. Phys. Chem. B 102, 8100 (1998).
N.J. Freeman, L.L. Peel, M.J. Swann, G.H. Cross, A. Reeves, S. Brand, J.R. Lu, J. Phys.: Condens. Matter 16, 2493 (2004).
C.E. Giacomelli, W. Norde, J. Colloid Interface Sci. 233, 234 (2001).
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Teichroeb, J.H., McVeigh, P.Z. & Forrest, J.A. Influence of nanoparticle size on the pH-dependent structure of adsorbed proteins studied with quantitative localized surface plasmon spectroscopy. Eur. Phys. J. E 30, 157–164 (2009). https://doi.org/10.1140/epje/i2008-10393-4
Received:
Revised:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1140/epje/i2008-10393-4