Abstract:
Interactions between isolated nucleosome core particles are studied as a function of the monovalent salt concentration by osmometry and by electrophoretic mobility measurements. The data are compared to the measurements performed on the protein-free DNA fragments and also analysed using the conventional theoretical approach. At low salt, an electrostatic screening effect accounts for the variation of the second virial coefficient whereas the simple hard-core contribution becomes predominant at high salt. In the intermediate range, an attraction occurs. In the light of previous results (Mangenot et al. Biophys. J. 82, 345 (2002)), we show that the flexible basic proteic tails are responsible for this attraction. A tail-bridging effect is discussed.
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Received 4 October 2001
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Mangenot, S., Raspaud, E., Tribet, C. et al. Interactions between isolated nucleosome core particles: A tail-bridging effect?. Eur. Phys. J. E 7, 221–231 (2002). https://doi.org/10.1140/epje/i200101151
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DOI: https://doi.org/10.1140/epje/i200101151