Abstract—
The results of a study of the structural and functional characteristics of phytoplankton in the coastal region of the Black Sea using flow cytometry are presented. The data on the seasonal variability of the biomass of three algal groups (Synechococcus, pico-eukaryotic phytoplankton, and nanophytoplankton), chlorophyll a content, percentage of living cells, and FDA (diacetate fluorescein) fluorescence characterizing the functional state of algae are obtained. A significantly positive relationship is found between the values (biomass and autofluorescence of chlorophyll) determined on the flow cytometer and the total content of chlorophyll a, calculated using standard methods. The effect of temperature, illumination, and content of nutrients in water on the biomass and the FDA fluorescence of three isolated groups of algae is shown. The nitrate content and temperature have no significant effect on the abundance of pico and nanophytoplankton, while a reliable relationship is established between the biomass of nanophytoplankton and the concentrations of dissolved forms of mineral phosphorus. An inverse statistically significant correlation is found between the light intensity and the biomass of picoeukaryotic phytoplankton. It is demonstrated that the abiotic environmental factors considered in the study do not significantly affect the FDA fluorescence value, except for temperature: in the warm period of the year, the picophytoplankton are most active in the Black Sea, while the cold period of the year is favorable for the development of nanophytoplankton.
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REFERENCES
Agawin, N.S.R., Duarte, C.M., and Agusti, S., Growth and abundance of Synechococcus sp. in a Mediterranean Bay: seasonality and relationship with temperature, Mar. Ecol.: Prog. Ser., 1998, vol. 170, pp. 45–53.
Agawin, N.S.R., Duarte, C.M., and Agusti, S., Nutrient and temperature control of the contribution of picoplankton to phytoplankton biomass and production, Limnol. Oceanogr., 2000, vol. 45, no. 3, pp. 591–600.
Bentley-Mowat, J.A. Application of fluorescence microscopy to pollution studies on marine phytoplankton, Bot. Mar., 1982, vol. 28, pp. 203–204.
Berglund, D.L. and Eversman, S., Flow cytometric measurement of pollutant stresses on algal cells, Cytometry, 1988, vol. 9, no. 2, pp. 150–155.
Burke, I.C., Lauenroth, W.K., and Parton, W.J., Regional and temporal variation in net primary production and nitrogen mineralization in grasslands, Ecology, 1997, vol. 78, no. 5, pp. 1330–1340.
Cloern, J.E. and Jassby, A.D., Patterns and scales of phytoplankton variability in estuarine–coastal ecosystems, Estuaries Coasts, 2010, vol. 33, no. 2, pp. 230–241.
Davey, H.M. and Kell, D.B., Flow cytometry and cell sorting of heterogeneous microbial populations: the importance of single-cell analyses, Microbiol. Mol. Biol. Rev., 1996, vol. 60, no. 4, pp. 641–696.
Dorsey, J., Yentsch, C.M., Mayo, S., and McKenna, C., Rapid analytical technique for the assessment of cell metabolic activity in marine microalgae, Cytometry Part A, 1989, vol. 10, no. 5, pp. 622–628.
Finenko, Z.Z., Churilova, T.Ya., and Suslin, V.V., Estimation of phytoplankton biomass and primary production in the Black Sea using satellite data, in Promyslovye bioresursy Chernogo i Azovskogo morei (Commercial Biological Resources of the Black Sea and Sea of Azov), Eremeev, V.N., Gaevskaya, A.V., Shul’man, G.E., and Zagorodnyaya, Yu.A., Eds., Sevastopol: EKOSI-Gidrofizika, 2011, pp. 220–236.
Finenko, Z.Z., Stel’makh, L.V., Mansurova, I.M., Georgieva, E.Yu., and Tsilinskii, V.S., Seasonal dynamics of structural and functional indicators of the phytoplankton community in the Sevastopol Bay, Sist. Kontrolya Okruzh. Sredy, 2017, vol. 9, no. 29, pp. 73–83.
Garvey, M., Moriceau, B., and Passow, U., Applicability of the FDA assay to determine the viability of marine phytoplankton under different environmental conditions, Mar. Ecol.: Prog. Ser., 2007, vol. 352, pp. 17–26.
Gilbert, F., Galgani, F., and Cadiou, Y., Rapid assessment of metabolic activity in marine microalgae: application in ecotoxicological tests and evaluation of water quality, Mar. Biol., 1992, vol. 112, no. 2, pp. 199–205.
Glover, H.E., Keller, M.D., and Spinrad, R.W., The effects of light quality and intensity on photosynthesis and growth of marine eukaryotic and prokaryotic phytoplankton clones, J. Exp. Mar. Biol. Ecol., 1987, vol. 105, nos. 2–3, pp. 137–159.
Heldal, M., Scanlan, D.J., Norland, S., Thingstad, F., and Mann, N.H., Elemental composition of single cells of various strains of marine Prochlorococcus and Synechococcus using X-ray microanalysis, Limnol. Oceanogr., 2003, vol. 48, no. 5, pp. 1732–1743.
Jeffrey, S.W. and Humphrey, G.F., New spectrophotometric equations for determining chlorophylls a, b, c1 and c2 in higher plants, algae and natural phytoplankton, Biochem. Physiol. Pflanzen., 1975, vol. 167, pp. 191–194.
Jochem, F.J., Morphology and DNA content of bacterioplankton in the northern Gulf of Mexico: analysis by epifluorescence microscopy and flow cytometry, Aquat. Microb. Ecol., 2001, vol. 25, no. 2, pp. 179–194.
Koblents-Mishke, O.I., Photosynthetic primary production, in Biologicheskie resursy okeana (Biological Resources of an Ocean), Moscow, 1985, pp. 48–62.
Latour, D., Giraudet, H., and Berthon, J.L., Frequency of dividing cells and viability of Microcystis aeruginosa in sediment of a eutrophic reservoir, Aquat. Microb. Ecol., 2004, vol. 36, no. 2, pp. 117–122.
Marie, D., Simon, N., and Vaulot, D., Phytoplankton cell counting by flow cytometry, Algal Cult. Techn., 2005, vol. 1, pp. 253–267.
McQuoid, M.R., Godhe, A., and Nordberg, K., Viability of phytoplankton resting stages in the sediments of a coastal Swedish fjord, Eur. J. Phycol., 2002, vol. 37, no. 2, pp. 191–201.
Mikaelyan, A.S., Silkin, V.A., and Pautova, L.A., Coccolithophorids in the Black Sea: their interannual and long-term changes, Oceanology (Engl. Transl.), 2011, vol. 51, no. 1, pp. 39–48.
Mukhanov, V.S., Rylkova, O.A., Churilova, T.Ya., Sakhon, E.G., and Pimenov, N.V., Structure and seasonal trophodynamics of picophytoplankton in Sevastopol bay and adjacent waters (the Black Sea), Microbiology (Moscow), 2016, vol. 85, no. 5, pp. 553–561.
Oradovskii, S.G., RD52.10.243-293. Rukovodstvo po khimicheskomu analizu morskikh vod (RD52.10.243-293. Manual for Chemical Analysis of Marine Waters), St. Petersburg: Gidrometeoizdat, 1993.
Osadchaya, T.S., Characterization of the phytoplankton dimensional structure from measurements of chlorophyll a, Ekol. Morya, 2007, vol. 73, pp. 70–74.
Parsons, T., Takahashi, M., and Hargrave, B., Biological Oceanographic Processes, Oxford: Pergamon, 1984, 2nd ed.
Partensky, F., Blanchot, J., and Vaulot, D., Differential distribution and ecology of Prochlorococcus and Synechococcus in oceanic waters: a review, Bull. Inst. Oceanogr. Monaco-Numero Spec., 1999, pp. 457–476.
Pick, F.R. and Caron, D.A., Picoplankton and nanoplankton biomass in Lake Ontario: relative contribution of phototrophic and heterotrophic communities, Can. J. Fish. Aquat. Sci., 1987, vol. 44, no. 12, pp. 2164–2172.
Shalapenok, L.S. and Shalapenok, A.A., Heterogeneous pigment composition of phycoerythrin-containing picocyanobacteria Synechococcus spp. in the Black Sea, Microbiology (Moscow), 1997, vol. 66, no. 1, pp. 80–84.
Shapiro, J., Current beliefs regarding dominance by blue-greens: the case for the importance of CO2 and pH, Mitt.-Int. Ver. Theor. Angew. Limnol., 1990, vol. 24, no. 1, pp. 38–54.
Sicko-Goad, L., Stoermer, E.F., and Kociolek, J.P., Diatom resting cell rejuvenation and formation: time course, species records and distribution, J. Plankton Res., 1989, vol. 11, no. 2, pp. 375–389.
Simon, M., Glöckner, F.O., and Amann, R., Different community structure and temperature optima of heterotrophic picoplankton in various regions of the Southern Ocean, Aquat. Microb. Ecol., 1999, vol. 18, no. 3, pp. 275–284.
Solomonova, E.S., Dynamics of physiologically active pico-and nanophytoplankton cells in the coastal waters of the Black Sea, Vestn. S.-Peterb. Univ., Ser. 3: Biol., 2016, vol. 1, pp. 62–72.
Solomonova, E.S. and Akimov, A.I., Evaluation of the functional state of the culture of Chlorella vulgaris Suboblonga using flow cytometry and variable fluorescence, Morsk. Ekol. Zh., 2012, vol. 4, pp. 78–84.
Solomonova, E.S. and Mukhanov, V.S., Flow cytometry for the assessment of the physiological active cells in batch cultures of Phaeodactylum tricornutum and Nitzschia sp., Morsk. Ekol. Zh., 2011, vol. 10, pp. 67–72.
Stel’makh, L.V., The contribution of picoplankton to the primary production and chlorophyll a content in eutrophic waters on the example of the Sevastopol Bay, Okeanologiya (Moscow), 1988, vol. 28, no. 1, pp. 127–131.
Stel’makh, L.V., Senicheva, M.I., and Babich, I.I., The ecological and physiological basis of the water blooming caused by Emiliania huxleyi in the Sevastopol Bay, Ekol. Morya, 2009, vol. 77, pp. 28–32.
Takahashi, M., Ichimura, S., Kishino, M., and Okami, N., Shade and chromatic adaptation of phytoplankton photosynthesis in a thermally stratified sea, Mar. Biol., 1989, vol. 100, no. 3, pp. 401–409.
Uysal, Z., Chroococcoid cyanobacteria Synechococcus spp. In the Black Sea: pigments, size, distribution, growth and diurnal variability, J. Plankton Res., 2001, vol. 23, pp. 175–190.
Uysal, Z., Pigments, size and distribution of Synechococcus spp. in the Black Sea, J. Mar. Syst., 2000, vol. 24, no. 3, pp. 313–326.
Verity, P.G., Robertson, C.Y., Tronzo, C.R., Andrews, M.G., Nelson, J.R., and Sieracki, M.E., Relationships between cell volume and the carbon and nitrogen content of marine photosynthetic nanoplankton, Limnol. Oceanogr., 1992, vol. 37, no. 7, pp. 1434–1446.
Wang, Z.H., Fu, Y.H., Kang, W., Liang, J.F., Gu, Y.G., and Jiang, X.L., Germination of phytoplankton resting cells from surface sediments in two areas of the Southern Chinese coastal waters, Mar. Ecol., 2013, vol. 34, no. 2, pp. 218–232.
Worden, A.Z., Nolan, J.K., and Palenik, B., Assessing the dynamics and ecology of marine picophytoplankton: the importance of the eukaryotic component, Limnol. Oceanogr., 2004, vol. 49, no. 1, pp. 168–179.
Wyman, M., An in vivo method for the estimation of phycoerythrin concentrations in marine cyanobacteria (Synechoccus spp.), Limnol. Oceanogr., 1992, vol. 37, no. 6, pp. 1300–1306.
Zaika, V.E., Shevchenko, V.A., and Bulatov, K.V., Ekologiya morskogo fototrofnogo pikoplanktona (Ecology of Marine Phototrophic Picoplankton), Moscow: Nauchn. Tsentr Biol. Issled., Akad. Nauk SSSR, 1989.
Zaika, V.E., Shalapenok, L.S., and Pokotilov, S.L., Izmenenie chastoty delyashchikhsya tsianobakterii po glubine i vremeni sutok (Changes in the Frequency of Dividing Cyanobacteria by the Depth and Time of Day), Available from VINITI, 1991, Sevastopol, no. 870-V91.
ACKNOWLEDGMENTS
I am grateful to I.M. Mansurova, a researcher of the Department of Ecological Physiology of Algae, Kovalevsky Institute of Marine Biological Research, Russian Academy of Sciences, for kindly presenting data on the chlorophyll а concentration and researchers at the Department of Aquaculture and Marine Pharmacology for determining the content of nutrients.
Funding
This study was performed within the framework of a state assignment of the Kovalevsky Institute of Marine Biological Research on the theme “Functional, Metabolic, and Toxicological Aspects of the Existence of Hydrobionts and Their Populations in Habitats with Different Physical and Chemical Regimes (project no. 0828-0003).
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Solomonova, E.S. Structural and Functional Characteristics of the Phytoplankton Community in Coastal Waters of the Black Sea. Contemp. Probl. Ecol. 12, 473–481 (2019). https://doi.org/10.1134/S199542551905010X
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DOI: https://doi.org/10.1134/S199542551905010X