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Direct electrochemistry of cytochrome c entrapped in agarose hydrogel by protein film voltammetry

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Abstract

Cytochrome c (cyt-c) entrapped in agarose hydrogel was electrostatically bound to the edge plane pyrolytic graphite electrode (EPPGE), and then stable agarose-cyt-c/EPPGE was prepared. Direct electrochemistry between cyt-c and the EPPGE was monitored by protein film voltammetry. The effects of ionic strength, pH and exterior substances on the direct electrochemistry, and electrocatalytic properties of cyt-c were also studied. Results show that the electrochemical behavior of heme Fe(III)/Fe(II) in cyt-c is quasireversible. Its electrochemical performance is the best when the concentration of supporting electrolyte phosphate buffer solution is 0.2 mol/l and pH is 7.0, and greatly inhibited by exterior metal ions. Catalytic reduction of oxygen could also be achieved at the agarose-cyt-c/EPPGE.

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Authors and Affiliations

  1. Faculty of Materials Science and Chemical Engineering, the State Key Laboratory Base of Novel Functional Materials and Preparation Science, Ningbo University, Ningbo, 315211, China

    Zhiyong Guo, Huina Zhang, Panpan Gai & Jing Duan

Authors
  1. Zhiyong Guo
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  2. Huina Zhang
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  3. Panpan Gai
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  4. Jing Duan
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Corresponding author

Correspondence to Zhiyong Guo.

Additional information

Published in Russian in Elektrokhimiya, 2011, Vol. 47, No. 2, pp. 188–194.

The article is published in the original.

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Guo, Z., Zhang, H., Gai, P. et al. Direct electrochemistry of cytochrome c entrapped in agarose hydrogel by protein film voltammetry. Russ J Electrochem 47, 175–180 (2011). https://doi.org/10.1134/S1023193511020108

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