Skip to main content
SpringerLink
Log in

A Study on the Effect of Prolactin Gene Variants on Milk Production Traits of Holstein Cattle

  • ANIMAL GENETICS
  • Published:
Russian Journal of Genetics Aims and scope Submit manuscript

Abstract

Recent studies aiming to improve the daily milk production of cattle using both genetic and environmental factors are extremely important. The aim of this study is to determine the possible relationships between daily milk production and polymorphic distribution of exon 3 region of the prolactin (PRL) gene, which is an important marker for selection. With this purpose, 155 Holstein cows were included in the study, and the demographic data of the cattle were recorded. Venous blood was collected from the jugular vein of cows in 2 tubes of 2 mL each containing EDTA for DNA isolation. Genomic DNA were isolated from these whole blood samples. The prolactin gene region of the cattle were identified using PCR/RFLP and Sanger DNA sequencing method. The genotype, allele frequencies and Hardy–Weinberg equilibrium were calculated using Arlequin version 3.5.1.3 package software. Accordingly, it was found that cows with genotype AA had statistically higher milk yield as compared to those with genotype AB and BB. Missense mutation, frameshift and silent mutations were detected in the prolactin gene exon 3. It was also found that cows with missense mutation had statistically lower milk fat percentage as compared to the others. Consequently, the effects of genotypic distribution and mutation in the prolactin gene on the milk production of Holstein cows, which is a special breed in terms of milk productivity, have been revealed.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1.

Similar content being viewed by others

REFERENCES

  1. Walsh, B., Minireview: quantitative genetics in the age of genomics, Theor. Popul. Biol., 2000, vol. 59, no. 3, pp. 175—184.

    Article  Google Scholar 

  2. Tambasco, D.D., Paz, C.C.P., Tambasco-Studart, M.D., et al., Candidate genes for growth traits inbeef cattle crosses Bos taurus × Bos indicus, J. Anim. Breed. Genet., 2003, vol. 120, pp. 51—56.

    Article  CAS  Google Scholar 

  3. Ponchon, B., Zhao, X., Ollier, S., et al., Lacasse relationship between glucocorticoids and prolactin during mammary gland stimulation in dairy cows, J. Dairy Sci., 2017, vol. 100, no. 2, pp. 1521—1534.

    Article  CAS  PubMed  Google Scholar 

  4. Cao, X., Wang, Q., Yan, J.B., et al., Molecular cloning and analysis of bovine prolactin full-long genomic as well as cDNA sequences, Yi Chuan Xue Bao, 2002, vol. 29, no. 9, pp. 768—773.

    CAS  PubMed  Google Scholar 

  5. Horseman, N.D., Zhao, W., Montecino-Rodriguez, E., et al., Defective mammopoiesis, but normal hematopoiesis, in mice with a targeted disruption of the prolactin gene, 1997, EMBO J., 1997, vol. 16, no. 23, pp. 6926—6935.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  6. Kim, J.H., Choi, B.H., and Lim, H.T., Characterization of phosphoinositide-3-kinase, class 3 (PIK3C3) gene and association tests with quantitative traits in pigs, Asian-Australasian J. Anim. Sci., 2005, vol. 18, no. 12, pp. 1701—1707.

    Article  CAS  Google Scholar 

  7. Yoon, D.H., Cho, B.H, Park, B.L., et al., Highly polymorphic bovine leptin gene, Asian-Australasian J. Anim. Sci., 2005, vol. 18, no. 11, pp. 1548—1551.

    Article  CAS  Google Scholar 

  8. Alipanah, M., Kalashnikova, L., and Rodionov, G., Association of prolactin gene variants with milk production traits in Russian Red Pied cattle, Iran. J. Biotech., 2007, vol. 5, no. 3, pp. 158—161.

    CAS  Google Scholar 

  9. Chrenek, P., Huba, J., Oravcova, M., et al., Genotypes of bGH and bPRL genes in relationships to milk production, in Book of Abstracts of the Annual Meeting—European Association for Animal Production, Zuerich: 1999, p. 40.

  10. Zeynep, S. and Memiş, Ö., Doğu Anadolu Kırmızısı Melez Sığırlarda, Prolaktin Geni Polimorfizmi1, Atatürk Üniv. Ziraat Fak. Derg., 2015, vol. 46, no. 2, pp. 119—123.

    Google Scholar 

  11. Bilal, A., Korhan, A., and Davut, B., Allelic frequency of kappa-casein, growth hormone and prolactin gene in Holstein, Brown Swiss and Simmental cattle breeds in Turkey, Kafkas Üniv. Vet. Derg., 2013, vol.19, no. 3, pp. 439—444.

    Google Scholar 

  12. Carpi, F.M., Di-Pietro, F., Vincenzetti, S., et al., Human DNA extraction methods: patents and applications, Recent Pat DNA Gene Seq., 2011, vol. 5, no. 1, pp. 1—7.

    Article  CAS  PubMed  Google Scholar 

  13. Mitra, A., Schlee, P, and Balakrishnan, C.R., Polymorphisms at growth-hormone and prolactin loci in Indian cattle and buffalo, J. Anim. Breed. Genet., 1995, vol. 112, nos. 1–6, pp. 71—74.

    Article  Google Scholar 

  14. Uddin, R.M., Babar, M.E., and Nadeem, A., Genetic analysis of prolactin gene in Pakistani cattle, Mol. Biol. Rep., 2013, vol. 40, no. 10, pp. 5685—5689.

    Article  CAS  PubMed  Google Scholar 

  15. Kumar, S., Nei, M., Dudley, J., et al., MEGA: a biologist-centric software for evolutionary analysis of DNA and protein sequences, Brief. Bioinf., 2008, vol. 9, no. 4, pp. 299—306.

    Article  CAS  Google Scholar 

  16. Memiş, Ö. and Ünsal, D., Sığırların Verim Özellikleri Üzerine Etkili Önemli Moleküler Markörler, Atatürk Üniv. Ziraat Fak. Derg., 2008, vol, 39, no. 1, pp. 127—135.

    Google Scholar 

  17. Hallerman, E.M., Theilmann, J.L., Beckmann, J.S., et al., Mapping of bovine prolactin and rhodopsin genes in hybrid somatic cells, Anim. Genet., 1998, vol. 19, no. 2, pp. 123—131.

    Article  Google Scholar 

  18. Klauzinska, M., Polymorphism of 5'-Flanking Regions of Genes GH, GHRH, Prolactin, and Cattle Miostatin, Poland: Inst. Anim. Genet. Breed., 2002.

    Google Scholar 

  19. Citek, J., Rehout, V., Neubauerova, V., Allele frequency at PRL (prolactin) and LGB (lactoglobulin beta) genes in Red cattle breeds from Central Europe and in other breeds, Czech J. Anim. Sci., 2001, vol. 46, no.10, pp. 433—438.

    CAS  Google Scholar 

  20. Miceikiene, I., Peciulaitiene, N., Baltrenaite, I., et al., Association of cattle genetic markers with performance traits, Biologija, 2006, vol. 1, pp. 24—29.

    Google Scholar 

  21. Citek, J., Rehout, V., Neubauerova, V., Allele frequency at PRL (prolactin) and LGB (lactoglobulin beta) genes in Red cattle breeds from Central Europe and in other breeds, Czech J. Anim. Sci., 2001, vol. 46, no. 10, pp. 433—438.

    CAS  Google Scholar 

  22. Dybus, A., Grzesiak, W., Kamieniecki, H., et al., Association of genetic variants of bovine prolactin with milk production traits of Black-and-White and Jersey cattle, Arch. Anim. Breed., 2005, vol. 48, no. 2, pp. 149—156.

    Article  CAS  Google Scholar 

  23. Zhou, G., Zhu, Q., Wu, Y., Polymorphism of PRL gene and its relationship with milk production traits in cows, J. J. Agric.Univ., 2006, vol. 28, no. 1, pp. 80—83.

    CAS  Google Scholar 

  24. Jaan, O.C., Ibeagha-Awemu, E.M., Özbeyaz, C., et al., Geographic distribution of haplotype diversity at the bovine casein locus, Genet. Sel. Evol., 2004, vol. 36, no. 2, pp. 243—257.

    Article  Google Scholar 

  25. Bilal, A., Korhan, A., Davut, B., et al., Allelic frequency of kappa-casein, growth hormone and prolactin gene in Holstein, Brown Swiss and Simmental cattle breeds in Turkey, Kafkas Üniv. Vet. Derg., 2013, vol. 19, no. 3, pp. 439—444.

    Google Scholar 

  26. Yousef, M., Cyrus, A., Mortaza, B., et al., Effects of bovine prolactin gene polymorphism within exon 4 on milk related traits and genetic trends in Iranian Holstein bulls, Afr. J. Biotechnol., 2009, vol. 8, no. 19, pp. 4797—4801.

    Google Scholar 

  27. Chung, E.R., Rhin, T.J., and Han, S.K, Association between PCR RFLP markers of growth hormone and prolactin genes and production traits in dairy cattle, Korean J. Anim. Sci., 1996, vol. 38, pp. 321—336.

    CAS  Google Scholar 

  28. Dybus, A., Associations of growth hormone GH and prolactin PRL genes polymorphism with milk production traits in Polish Black and White cattle, Anim. Sci. Pap. Rep., 2002, vol. 20, no. 4, pp. 203—212.

    CAS  Google Scholar 

  29. Brym, P., Kamiñski, S., and Wójcik, E., Nucleotide sequence polymorphism within exon 4 of the bovine prolactin gene and its associations with milk performance traits, J. Appl. Genet., 2005, vol. 45, no. 2, pp. 179—185.

    Google Scholar 

  30. Li, J.T., Wang, A.H., Chen, P., et al., Relationship between the polymorphisms of 5'regulation region of prolactin gene and milk traits in Chinese Holstein dairy cows, Asian-Australasian J. Anim. Sci., 2006, vol. 19, no. 4, pp. 459—462.

    Article  Google Scholar 

  31. Mathew, D.L., Kristen, M.H., Kathryn, T., et al., Functionally reciprocal mutations of the prolactin signalling pathway define hairy and slick cattle, Nat. Commun., 2014, vol. 18, no. 5, pp. 5861—5869.

    Google Scholar 

  32. Feng, H., Dongxiao, S., Ying, Y., et al., Association between SNPs within prolactin gene and milk performance traits in Holstein dairy cattle, Asian-Australasian J. Anim. Sci., 2006, vol. 19, no. 10, pp. 1384—1389.

    Article  Google Scholar 

Download references

ACKNOWLEDGMENTS

In this study, Gaziantep University BAP-2017.SHMYO 17.01 number is supported by Scientific Research Projects I would like to thank for their support at this point.

Author information

Authors and Affiliations

  1. Department of Medical Services and Techniques, Vocational School of Health Services, University of Gaziantep, 27310, Gaziantep, Turkey

    S. Bayıl Oğuzkan

  2. Department of Plant and Animal Production, Technical Services, University of Gaziantep, 27310, Gaziantep, Turkey

    A. S. Bozkurt

Authors
  1. S. Bayıl Oğuzkan
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. A. S. Bozkurt
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to S. Bayıl Oğuzkan.

Ethics declarations

Conflict of interests. The authors declare that they have no conflict of interest.

Statement on the welfare of animals. All applicable international, national, and/or institutional guidelines for the care and use of animals were followed.

Additional information

The article is published in the original.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Bayıl Oğuzkan, S., Bozkurt, A.S. A Study on the Effect of Prolactin Gene Variants on Milk Production Traits of Holstein Cattle. Russ J Genet 55, 480–486 (2019). https://doi.org/10.1134/S1022795419040082

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1134/S1022795419040082

Keywords:

Search

Navigation