Abstract
A total of 129 Limousin calves were used to investigate how leptin gene polymorphisms affect growth traits, such as body weight, average daily gain, wither height, sacrum height and chest girth in beef cattle. Two single nucleotide polymorphisms (SNPs) were genotyped, including the Sau3AI polymorphism in intron 2 and the A59V polymorphism in exon 3. The genotype and allele frequencies for each SNP and the haplotype frequencies for both SNPs were estimated in the studied herd. Statistical analysis revealed that the A59V polymorphism significantly affected the body weight at 210 days of age (P ≤ 0.01) and the average daily gain between 3 and 210 days of age (P ≤ 0.05) with T as a desirable allele. No associations were observed between the Sau3AI polymorphism and the growth traits mentioned above. However, the average daily gain between 3 and 210 days of age was significantly higher in the CT/CT haplotype animals compared with the CC/CC and CC/CT individuals. These results indicated that selection for the A59V TT animals might contribute to an improved body weight in Limousin cattle.
Similar content being viewed by others
References
Zhang, Y., Proenca, R., Maffei, M., et al., Positional Cloning of the Mouse obese Gene and Its Human Homologue, Nature, 1994, vol. 372, pp. 425–432.
Houseknecht, K.L., Baile, C.A., Matteri, R.L., and Spurlock, M.E., The Biology of Leptin: A Review, J. Anim. Sci., 1998, vol. 76, pp. 1405–1420.
Delavaud, C., Ferlay, A., Faulconnier, Y., et al., Plasma Leptin Concentration in Adult Cattle: Effects of Breed, Adiposity, Feeding Level, and Meal Intake, J. Anim. Sci., 2002, vol. 80, pp. 1317–1328.
Leshin, L.S., Barb, C.R., Kiser, T.E., et al., Growth Hormone-Releasing Hormone and Somatostatin Neurons within the Porcine and Bovine Hypothalamus, Neuroendocrinology, 1994, vol. 59, pp. 251–264.
Barb, C.R., Yan, X., Azain, M.J., et al., Recombinant Porcine Leptin Reduces Feed Intake and Stimulates Growth Hormone Secretion in Swine, Domest. Anim. Endocrinol., 1998, vol. 15, pp. 77–86.
Nkrumah, J.D., Li, C., Basarab, J.B., et al., Association of a Single Nucleotide Polymorphism in the Bovine Leptin Gene with Feed Intake, Feed Efficiency, Growth, Feeding Behaviour, Carcass Quality and Body Composition, Can. J. Anim. Sci., 2004, vol. 84, pp. 211–219.
Pomp, D., Zou, T., Clutter, A.C., and Barendse, W., Rapid Communication: Mapping of Leptin to Bovine Chromosome 4 by Linkage Analysis of a PCR-Based Polymorphism, J. Anim. Sci., 1997, vol. 75, p. 1427.
Zwierzchowski, L., Oprzadek, J., Dymnicki, E., and Dzierzbicki, P., An Association of Growth Hormone, κ-Casein, β-Lactoglobulin, Leptin and Pit-1 Loci Poly morphism with Growth Rate and Carcass Trait in Beef Cattle, Anim. Sci. Papers Rep., 2001, vol. 19, pp. 65–78.
Oprzadek, J., Flisikowski, K., Zwierzchowski, L., and Dymnicki, E., Polymorphisms at Loci of Leptin (LEP), Pit-1 and STAT5A and Their Association with Growth, Feed Conversion and Carcass Quality in Black-and-White Bulls, Anim. Sci. Papers Rep., 2003, vol. 21, pp. 135–145.
Nkrumah, J.D., Li, C., Yu, J., et al., Polymorphisms in the Bovine Leptin Promoter Associated with Serum Leptin Concentration, Growth, Feed Intake, Feeding Behavior, and Measures of Carcass Merit, J. Anim. Sci., 2005, vol. 83, pp. 20–28.
Haegeman, A., Van Zeveren, A., and Peelman, L.J., New Mutation in Exon 2 of the Bovine Leptin Gene, Anim. Genet., 2000, vol. 31, p. 79.
STATISTICA for Windows (Data Analysis Software System), Version 7.1, StatSoft: Tulsa, 2006.
Nkrumah, J.D., Li, C., Keisler, D.H., et al., Polymorphisms in the Leptin Gene and Their Associations with Performance, Feed Efficiency, and Carcass Merit of Beef Cattle, in Genetics Applied to Livestock Production, Proc. 8th World Congress, Belo Horizonte, 2006.
Author information
Authors and Affiliations
Corresponding author
Additional information
The article is published in the original.
Rights and permissions
About this article
Cite this article
Kulig, H., Kmieć, M. Association between leptin gene polymorphisms and growth traits in Limousin cattle. Russ J Genet 45, 738–741 (2009). https://doi.org/10.1134/S1022795409060131
Received:
Published:
Issue Date:
DOI: https://doi.org/10.1134/S1022795409060131