Abstract
The molecular genetic, morphometric, and physiological-biochemical characteristics of three isolates of the carotenogenic microalga Haematococcus sp. obtained in different regions of the Caucasus and Crimea were studied. According to the results of light microscopy and PCR analysis of 18s rDNA gene fragments, all isolates were identified as Haematococcus pluvialis (clade Chlorogonia, order Volvocales, class Chlorophyceae). It is shown that the strains isolated in the mountainous and valley areas of the Black Sea region differ in important characteristics relevant for mass cultivation: cell size, growth rate, stress tolerance, mass fraction of total carotenoids and astaxanthin in dry matter, and their yield per liter of the start culture. The H. pluvialis isolates most promising for further research and the development of a cultivation technology for the Black Sea regions of the Caucasus and Crimea were strains IMBR-3 (Sevastopol) and IMBR-1 (Adler), which were characterized by the highest astaxanthin production (16.77 ± 1.32 and 17.12 ± 0.72 mg/(L day), respectively).
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REFERENCES
Han, D., Li, Y., and Hu, Q., Handbook of Microalgal Culture: Applied Phycology and Biotechnology, Richmond, A. and Hu, Q., Eds., New York: :John Wiley and Sons, 2013, pp. 388–405.
Shah, M.M.R., Liang, Y., Cheng, J.J., and Daroch, M., Front. Plant Sci., 2016, vol. 7, art. 531, pp. 1–28. doi 10.3389/fpls.2016.00531
Moretti, V.M., Mentasti, T., Bellagamba, F., Luzzana, U., Caprino, F., Turchini, G.M., Giani, I., and Valfre, F., Food. Add. Contam., 2006, vol. 23, pp. 1056–1063.
Noroozi, M., Omar, H., Napis, S., Hejazi, M.A., and Tan, S.G., African J. Biotechnol., 2012, vol. 11, no. 84, pp. 15049–15059.
Gonzalez, M.A., Cifuentes, A.S., and Gomez, P.I., Gayana. Botanica, 2009, vol. 66, no. 1, pp. 58–70.
Gao, Z., Meng, C., Chen, Y.C., Ahmed, F., Mangott, A., Schenk, P.M., and Li, Y., J. Appl. Phycol., 2015, vol. 27, no. 5, pp. 1853–1860.
Minyuk, G.S., Terent’eva, N.V., and Drobetskaya, I.V., Algologiya, 2007, vol. 17, no. 2, pp. 148–159.
Fabregas, J., Otero, A., Maseda, A., and Dominguez, A., J. Biotechnol., 2001, vol. 89, pp. 65–71.
Andersen, R.A., Algal Culturing Techniques, San Diego: Academic Press, 2005.
Buchheim, M.A., Sutherland, D.M., Buchheim, J.A., and Wolf, M., Eur. J. Phycol., 2013, vol. 48, no. 3, pp. 318–329.
Tamura, K., Stecher, G., Peterson, D., Filipski, A., and Kumar, S., Mol. Biol. EV, 2013, vol. 30, pp. 2725–2729.
Edgar, RC., Nucleic Acids Res., 2004, vol. 32, no. 5, pp. 1792–1797.
Aldrich, J.R.A., Stat. Sci., 1997, vol. 12, no. 3, pp. 162–176.
Kimura, M., J. Mol. Evol., 1980, vol. 16, pp. 111–120.
Felsenstein, J., Evolution, 1985, vol. 39, pp. 783–791.
Britton, G., in Eds.: Britton, G., Liaasen-Jensen, S., and Pfander, H., Carotenoids, vol. 1 A: Isolation and Analysis, Basel: Birkauser Verlag, 1995, pp. 199–225.
Lichtenthaler, H.K., Meth. Enzymol., 1987, vol. 148, pp. 350–382.
Drobetskaya, I.V., Chubchikova, I.N., Borovkov, A.B., and Minyuk, G.S., Ekol. Morya, 2010, vol. 79, pp. 50–56.
Pocock, M.A., Trans. R. Sos. S. Afr., 1960, vol. 36, no. 1, pp. 5–55.
Gu, W., Xie, X., Gao, S., Zhou, W., and Pan, G., PLoS One, 2013, vol. 8, no. 7.
Pentecost, A., in The Freshwater Algal Flora of the British Isles: An Identification Guide to Freshwater and Terrestrial Algae, John, D.M., Whitton, B.A., and Brook, A.J., Eds., Cambridge: CUP, 2002, vol. 1, pp. 381–409.
Klochkova, T.A., Kwak, M.S., Han, J.W., Motomura, T., Nagasato, C., and Kim, G.H., Algae, 2013, vol. 28, no. 2, pp. 185–192.
Chekanov, K., Lobakova, E., Selyakh, I., Semenova, L., Sidorov, R., and Solovchenko, A., Mar. Drugs, 2014, vol. 12, no. 8, pp. 4504–4520.
Elliot, F.M., Arch. Protistenk., 1934, vol. 82, pp. 250–273.
Zhang, C., Liu, J., and Zhang, L., Chinese J. Ocean. Limnol., 2017, vol. 35, no. 5, pp. 1205–1211.
Proschold, T. and Leliaert, F., in Systematics Association Special Volume Series, Brodie, J. and Lewis, J.M., Eds., Boca Raton, FL: CRC Press, 2007, pp. 123–153.
Triki, A., Maillard, P., and Gudin, C., Phycologia, 1997, vol. 36, no. 3, pp. 190–194.
Liu, J.G., Yin, M.Y., Zhang, J.P., Meng, Z.C., and Bourne, W.F., Oceanol. Limnol. Sinica, 2000, vol. 31, no. 2, pp. 145–150.
Masyuk, N.P. and Demchenko, E.N., Algologiya, 2001, vol. 11, no. 3, pp. 298–310.
Minyuk, G.S., Erokhin, V.E., Gordienko, A.P., Terent’eva, N.V., Chubchikova, I.N., and Drobetskaya, I.V., in Mikrovodorosli Chernogo morya: problemy sokhraneniya bioraznoobraziya i biotekhnologicheskogo ispol’zovaniya (Microalgae of the Black Sea: Problems of Biodiversity Conservation and Biotechnological Use), Sevastopol: EKOSI-Gidrofizika, 2008, pp. 353–392.
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Chelebieva, E.S., Dantsyuk, N.V., Chekanov, K.A. et al. Identification and Morphological-Physiological Characterization of Astaxanthin Producer Strains of Haematococcus pluvialis from the Black Sea Region. Appl Biochem Microbiol 54, 639–648 (2018). https://doi.org/10.1134/S0003683818060078
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DOI: https://doi.org/10.1134/S0003683818060078