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Identification and Morphological-Physiological Characterization of Astaxanthin Producer Strains of Haematococcus pluvialis from the Black Sea Region

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Abstract

The molecular genetic, morphometric, and physiological-biochemical characteristics of three isolates of the carotenogenic microalga Haematococcus sp. obtained in different regions of the Caucasus and Crimea were studied. According to the results of light microscopy and PCR analysis of 18s rDNA gene fragments, all isolates were identified as Haematococcus pluvialis (clade Chlorogonia, order Volvocales, class Chlorophyceae). It is shown that the strains isolated in the mountainous and valley areas of the Black Sea region differ in important characteristics relevant for mass cultivation: cell size, growth rate, stress tolerance, mass fraction of total carotenoids and astaxanthin in dry matter, and their yield per liter of the start culture. The H. pluvialis isolates most promising for further research and the development of a cultivation technology for the Black Sea regions of the Caucasus and Crimea were strains IMBR-3 (Sevastopol) and IMBR-1 (Adler), which were characterized by the highest astaxanthin production (16.77 ± 1.32 and 17.12 ± 0.72 mg/(L day), respectively).

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REFERENCES

  1. Han, D., Li, Y., and Hu, Q., Handbook of Microalgal Culture: Applied Phycology and Biotechnology, Richmond, A. and Hu, Q., Eds., New York: :John Wiley and Sons, 2013, pp. 388–405.

    Google Scholar 

  2. Shah, M.M.R., Liang, Y., Cheng, J.J., and Daroch, M., Front. Plant Sci., 2016, vol. 7, art. 531, pp. 1–28. doi 10.3389/fpls.2016.00531

  3. Moretti, V.M., Mentasti, T., Bellagamba, F., Luzzana, U., Caprino, F., Turchini, G.M., Giani, I., and Valfre, F., Food. Add. Contam., 2006, vol. 23, pp. 1056–1063.

    Article  CAS  Google Scholar 

  4. Noroozi, M., Omar, H., Napis, S., Hejazi, M.A., and Tan, S.G., African J. Biotechnol., 2012, vol. 11, no. 84, pp. 15049–15059.

    CAS  Google Scholar 

  5. Gonzalez, M.A., Cifuentes, A.S., and Gomez, P.I., Gayana. Botanica, 2009, vol. 66, no. 1, pp. 58–70.

    Article  Google Scholar 

  6. Gao, Z., Meng, C., Chen, Y.C., Ahmed, F., Mangott, A., Schenk, P.M., and Li, Y., J. Appl. Phycol., 2015, vol. 27, no. 5, pp. 1853–1860.

    Article  CAS  Google Scholar 

  7. Minyuk, G.S., Terent’eva, N.V., and Drobetskaya, I.V., Algologiya, 2007, vol. 17, no. 2, pp. 148–159.

    Google Scholar 

  8. Fabregas, J., Otero, A., Maseda, A., and Dominguez, A., J. Biotechnol., 2001, vol. 89, pp. 65–71.

    Article  CAS  PubMed  Google Scholar 

  9. Andersen, R.A., Algal Culturing Techniques, San Diego: Academic Press, 2005.

    Google Scholar 

  10. Buchheim, M.A., Sutherland, D.M., Buchheim, J.A., and Wolf, M., Eur. J. Phycol., 2013, vol. 48, no. 3, pp. 318–329.

    Article  Google Scholar 

  11. Tamura, K., Stecher, G., Peterson, D., Filipski, A., and Kumar, S., Mol. Biol. EV, 2013, vol. 30, pp. 2725–2729.

    Article  CAS  Google Scholar 

  12. Edgar, RC., Nucleic Acids Res., 2004, vol. 32, no. 5, pp. 1792–1797.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  13. Aldrich, J.R.A., Stat. Sci., 1997, vol. 12, no. 3, pp. 162–176.

    Article  Google Scholar 

  14. Kimura, M., J. Mol. Evol., 1980, vol. 16, pp. 111–120.

    Article  CAS  PubMed  Google Scholar 

  15. Felsenstein, J., Evolution, 1985, vol. 39, pp. 783–791.

    Article  PubMed  Google Scholar 

  16. Britton, G., in Eds.: Britton, G., Liaasen-Jensen, S., and Pfander, H., Carotenoids, vol. 1 A: Isolation and Analysis, Basel: Birkauser Verlag, 1995, pp. 199–225.

  17. Lichtenthaler, H.K., Meth. Enzymol., 1987, vol. 148, pp. 350–382.

    Article  CAS  Google Scholar 

  18. Drobetskaya, I.V., Chubchikova, I.N., Borovkov, A.B., and Minyuk, G.S., Ekol. Morya, 2010, vol. 79, pp. 50–56.

    Google Scholar 

  19. Pocock, M.A., Trans. R. Sos. S. Afr., 1960, vol. 36, no. 1, pp. 5–55.

    Article  Google Scholar 

  20. Gu, W., Xie, X., Gao, S., Zhou, W., and Pan, G., PLoS One, 2013, vol. 8, no. 7.

  21. Pentecost, A., in The Freshwater Algal Flora of the British Isles: An Identification Guide to Freshwater and Terrestrial Algae, John, D.M., Whitton, B.A., and Brook, A.J., Eds., Cambridge: CUP, 2002, vol. 1, pp. 381–409.

    Google Scholar 

  22. Klochkova, T.A., Kwak, M.S., Han, J.W., Motomura, T., Nagasato, C., and Kim, G.H., Algae, 2013, vol. 28, no. 2, pp. 185–192.

    Article  CAS  Google Scholar 

  23. Chekanov, K., Lobakova, E., Selyakh, I., Semenova, L., Sidorov, R., and Solovchenko, A., Mar. Drugs, 2014, vol. 12, no. 8, pp. 4504–4520.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  24. Elliot, F.M., Arch. Protistenk., 1934, vol. 82, pp. 250–273.

    Google Scholar 

  25. Zhang, C., Liu, J., and Zhang, L., Chinese J. Ocean. Limnol., 2017, vol. 35, no. 5, pp. 1205–1211.

    Article  Google Scholar 

  26. Proschold, T. and Leliaert, F., in Systematics Association Special Volume Series, Brodie, J. and Lewis, J.M., Eds., Boca Raton, FL: CRC Press, 2007, pp. 123–153.

    Google Scholar 

  27. Triki, A., Maillard, P., and Gudin, C., Phycologia, 1997, vol. 36, no. 3, pp. 190–194.

    Article  Google Scholar 

  28. Liu, J.G., Yin, M.Y., Zhang, J.P., Meng, Z.C., and Bourne, W.F., Oceanol. Limnol. Sinica, 2000, vol. 31, no. 2, pp. 145–150.

    Google Scholar 

  29. Masyuk, N.P. and Demchenko, E.N., Algologiya, 2001, vol. 11, no. 3, pp. 298–310.

    Google Scholar 

  30. Minyuk, G.S., Erokhin, V.E., Gordienko, A.P., Terent’eva, N.V., Chubchikova, I.N., and Drobetskaya, I.V., in Mikrovodorosli Chernogo morya: problemy sokhraneniya bioraznoobraziya i biotekhnologicheskogo ispol’zovaniya (Microalgae of the Black Sea: Problems of Biodiversity Conservation and Biotechnological Use), Sevastopol: EKOSI-Gidrofizika, 2008, pp. 353–392.

    Google Scholar 

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Authors and Affiliations

  1. Kovalevsky Institute of Marine Biological Research, Russian Academy of Sciences, 299011, Sevastopol, Russia

    E. S. Chelebieva, N. V. Dantsyuk, I. N. Chubchikova, I. V. Drobetskaya & G. S. Minyuk

  2. Lomonosov Moscow State University, 119991, Moscow, Russia

    K. A. Chekanov, E. S. Lobakova & A. E. Solovchenko

Authors
  1. E. S. Chelebieva
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  2. N. V. Dantsyuk
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  3. K. A. Chekanov
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  4. I. N. Chubchikova
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  5. I. V. Drobetskaya
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  6. G. S. Minyuk
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  7. E. S. Lobakova
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  8. A. E. Solovchenko
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Correspondence to A. E. Solovchenko.

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Translated by M. Batrukova

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Chelebieva, E.S., Dantsyuk, N.V., Chekanov, K.A. et al. Identification and Morphological-Physiological Characterization of Astaxanthin Producer Strains of Haematococcus pluvialis from the Black Sea Region. Appl Biochem Microbiol 54, 639–648 (2018). https://doi.org/10.1134/S0003683818060078

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  • DOI: https://doi.org/10.1134/S0003683818060078

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