Abstract
Using vesicles of symbiosome membrane (SM), it was shown that the Ca2+-ATPase can function as an ATP-energized Ca2+/H+ antiporter. The initial rate of the acidic shift inside the vesicles, as well as the rate of the ITP-dependent alkalization of the medium inside them markedly increased in the presence of valinomycin. This process was rapidly stopped by eosin Y, a known inhibitor of the type IIB Ca2+-ATPase. ITP-dependent uptake of Ca2+ was blocked after the addition to the reaction mixture of nigericin in the presence of K+. Under these conditions, the alkaline shift of pH inside the vesicles occurred, leading to the inhibition of operation of the calcium pump in SM. Evaluation of the pH shifts inside the vesicles by using pH-indicator pyranine confirmed the ion-exchange mechanism of the Ca2+-ATPase functioning in the SM.
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References
Roth L.E., Stacey G. 1989. Bacterium release into host cells of nitrogen-fixing soybean nodules: The symbiosome membrane comes from three sources. Eur. J. Cell Biol. 49, 13–23.
Brewin N.J. 1991. Development of the legume root nodule. Annu. Rev. Cell Biol. 7, 191–226.
Udvardi M., Day D.A. 2013. Transport and metabolism in legume-rhizobia symbiosis. Annu. Rev. Plant Biol. 64, 781–805.
Izmailov S.F. 2014. Simbiosomnaya membrana (Symbiosome membrane). Moscow: Nauka.
Bush D.S. 1995. Calcium regulation in plant cells and its role in signaling. Annu. Rev. Plant Physiol. Plant Mol. Biol. 46, 95–122.
Bose J., Pottosin I.I., Shabala S.S., Palmgren M.G., Shabala S. 2001. Calcium efflux systems in stress signaling and adaptation in plants. Front. Plant Sci. 2, 1–17.
Andreev I.M., Dubrovo P.N., Krylova V.V., Izmailov S.F. 1999. Functional identification of ATP-driven Ca2+ pump in the peribacteroid membrane of broad bean root nodules. FEBS Lett. 447, 49–52.
Andreeva I.N., Andreev I.M., Kozharinova G.M., Krylova V.V., Izmailov S.F. 1999. Calcium stores in symbiosomes from yellow lupin root nodules. J. Plant Physiol. 155, 357–363.
Andreev I.M., Dubrovo P.N., Krylova V.V., Izmailov S.F. 1998. Calcium uptake by symbiosomes and the peribacteroid membrane vesicles isolated from yellow lupin root nodules. J. Plant Physiol. 153, 203–211.
Krylova V.V., Andreev I.M., Zartdinova R., Izmailov S.F. 2013. Biochemical characterization of Ca2+-ATPase in the peribacteroid membrane of broad bean root nodules. Protoplasma. 250, 531–538.
Clarke V.C., Loughlin P.C., Garvin A., Chen C., Brear E.M., Day D.A., Smith M.C. 2014. Proteomic analysis of the soybean symbiosome identifies new symbiotic proteins. Mol. Cell Prot. 5, 1–9.
Niggli V., Sigel E. 2008. Anticipating antiport in P-type ATPases. Trends Biochem. Sci. 33, 156–160.
Tadini-Buoninsegni T., Bartolommei G., Moncelli M.R., Guidelli R., Inesi G. 2006. Presteady state electrogenic events of Ca2+/H+ exchange and transport by the Ca2+- ATPase. J. Biol. Chem. 281, 37720–37727.
Andreev I.M., Dubrovo P.N., Krylova V.V., Andreeva I.N., Koren’kov V.D., Sorokin E.M., Izmailov S.F. 1997. Characterization of ATP-hydrolyzing and ATP-driven proton-translocating activities associated with the peribacteroid membrane from root nodules of Lupinus luteus L. J. Plant Physiol. 151, 563–569.
Palmgren M.G. 1991. Acridine orange as a probe for measuring pH gradients across membranes: Mechanism and limitations. Anal. Biochem. 192, 316–321.
Kendrick N.C., Ratzlaff R.W., Blanstein M.P. 1977. Arsenazo III as an indicator for ionized calcium in physiological salt solutions: Its use for determination of the Ca-ATP dissociation constant. Anal. Biochem. 83, 433–450.
Bradford M.M. 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein dye binding. Anal. Biochem. 72, 248–254.
Andreev I.M., Krylova V., Dubrovo P., Izmailov S. 2005. Passive potassium transport by symbiosomes from broad bean root nodules. Plant Sci. 168, 1005–1010.
De Michelis M.I., Carnelli A., Rasi-Caldogno F. 1993. The Ca2+ pump of the plasma membrane of Arabidopsis thaliana: Characteristics and sensitivity to fluorescein derivatives. Botanica Acta. 106, 20–25.
Krylova V.V., Andreeva I.N., Zartdinova R. F., Izmailov S.F. 2014. Ca2+/H+ antiport as a possible mechanism the Ca2+-ATPase work in the symbiosome membrane. In: Interregional scientific collection Combination and regulation of physiological and biochemical processes. VGU, Issue 16, 71–77.
Tuteja N. 2009. Integrated calcium signaling in plants. In: Signaling in plants. Ed. Baluska F., Mancuso S. Berlin Heidelberg: Springer-Verlag, p. 29–51.
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Original Russian Text © V.V. Krylova, R.F. Zartdinova, I.M. Andreev, S.F. Izmailov, 2016, published in Biologicheskie Membrany, 2016, Vol. 33, No. 2, pp. 113–118.
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Krylova, V.V., Zartdinova, R.F., Andreev, I.M. et al. Ca2+/H+ antiport as a possible mechanism of the Ca2+-translocating ATPase functioning in vesicles of bean root nodule’s symbiosome membrane. Biochem. Moscow Suppl. Ser. A 10, 218–222 (2016). https://doi.org/10.1134/S1990747816010074
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DOI: https://doi.org/10.1134/S1990747816010074