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Parentage assignment in hatchery population of brown sole Pleuronectes herzensteini by microsatellite DNA markers

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Abstract

Five loci (Phz2, Phz6, Phz7, Phz12, and Phz14) of microsatellite DNA markers developed in a previous study for parentage assignment in the hatchery population generated by mating among 61 broodstock fish (35 females and 26 males) in a spawning tank, were selected. After natural spawning in the same tank, larvae collected at three different times were categorized into early phase (EP), middle phase (MP), and late phase (LP) groups. In the parental broodstock, the mean number of alleles per locus was 21.8 and expected heterozygosity (H E) was 0.813. In the progeny, the mean number of alleles per locus decreased to 11.6 (EP), 14.4 (MP), and 6.4 (LP) and H E to 0.796 (EP), 0.833 (MP), and 0.681 (LP). Parental assignment determined eight dams and six sires as major parents for the EP group. In the MP group, 13 dams and ten sires genetically contributed to spawning, but only three dams and two sires were involved in LP group progeny. In the hatchery population produced from a limited number of parental fish such as the LP group, genetic variability was apparently decreased.

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Author notes

  1. Sang -Gyu Kim

    Present address: Korea Inter-University Institute of Ocean Science, Pukyong National University, 599-1, Daeyeun, Namgu, 608-737, Busan, Korea

  2. Kagayaki Morishima

    Present address: Fisheries Laboratory, Kinki University, Shirahama, 649-2211, Wakayama, Japan

  3. Nobukazu Satoh & Setsuo Saito

    Present address: Hokkaido Mariculture Fisheries Experiment Station, 051-0013, Muroran, Hokkaido, Japan

  4. Takashi Fujioka

    Present address: Hokkaido Hakodate Fisheries Experiment Station, 042-0932, Hakodate, Hokkaido, Japan

Authors and Affiliations

  1. Graduate School of Fisheries Sciences, Hokkaido University, 041-8611, Hakodate, Hokkaido, Japan

    Sang -Gyu Kim, Kagayaki Morishima & Katsutoshi Arai

  2. Hokkaido Institute of Mariculture, 041-1404, Shikabe, Hokkaido, Japan

    Nobukazu Satoh, Takashi Fujioka & Setsuo Saito

Authors
  1. Sang -Gyu Kim
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  2. Kagayaki Morishima
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  4. Takashi Fujioka
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  5. Setsuo Saito
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  6. Katsutoshi Arai
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Correspondence to Katsutoshi Arai.

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Kim, S.G., Morishima, K., Satoh, N. et al. Parentage assignment in hatchery population of brown sole Pleuronectes herzensteini by microsatellite DNA markers. Fish Sci 73, 1087–1093 (2007). https://doi.org/10.1111/j.1444-2906.2007.01440.x

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  • DOI: https://doi.org/10.1111/j.1444-2906.2007.01440.x

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