Abstract
We have previously found that a ras switch 1 domain peptide (PNC-7, residues 35–47) selectively blocks oocyte maturation induced by oncogenic (Val 12–containing) ras-p21 protein and also blocks c-raf–induced oocyte maturation. We now find that oncogenic ras-p21 does not inhibit oocyte maturation of a constitutively activated raf protein (raf BXB) that is lacking most of the first 301 amino terminal amino acids, including the major ras binding domain and accessory ras-binding regions. We also find that a dominant negative raf that completely blocks c-raf–induced maturation likewise does not block raf-BXB–induced maturation. We conclude that PNC-7 blocks ras by binding to the amino-terminal domain of raf and that raf BXB must initiate signal transduction in the cytosol.
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REFERENCES
Adler, V., Pincus, M. R., Brandt-Rauf, P. W., and Ronai, Z. (1995). Proc. Natl. Acad. Sci. USA 92, 10585–10589.
Adler, V., Pincus, M. R., Polatskaya, A., Montano, X., Friedman, F. K., and Ronai, Z. (1996). J. Biol. Chem. 271, 23304–23309.
Chen, J. M., Monaco, R., Manolatos, S., Brandt-Rauf, P. W., Friedman, F. K., and Pincus, M. R. (1997). J. Prot. Chem. 16, 631–635.
Chen, J. M., Rijwani, K., Friedman, F. K., Hyde, M. J., and Pincus, M. R. (1999). J. Prot. Chem. 7, 543–549.
Chie, L., Chen, J. M., Friedman, F. K., Chung, D. L., Amar, S. Michl, J., Yamiazumi, Z., Brandt-Rauf, P. W., and Pincus, M. R. (1999). J. Prot. Chem. 18, 881–884.
Chie, L., Amar, S., Kung, H.-F., Lin, M. C. M., Chen, H., Chung, D., Adler, V., Ronai, Z., Friedman, F. K., Robinson, R. C., Kovac, C., Brandt-Rauf, P. W., Yamaizumi, Z., Michl, J., and Pincus, M. R. (2000). Cancer Chemother. Pharmacol. 45, 441–449.
Chung, D. L., Brandt-Rauf, P. W., Weinstein, I. B., Nishimura, S., Yamaizumi, Z., Murphy R. B., and Pincus, M. R. (1992) Proc. Natl. Acad. Sci. USA 89, 1993–1996.
Chung, D., Amar, S., Glozman, A., Chen, J. M., Friedman, F. K., Robinson, R., Monaco, R., Brandt-Rauf, P. W., Yamaizumi, Z., and Pincus, M. R. (1997). J. Prot. Chem. 16, 619–629.
Clark, J., Drugan, J. K., Terrell, R. S., Bradham, C., Der, C. J., Bell, R. M., and Campbell, S. (1996). Proc. Natl. Acad. Sci. USA 93, 1577–1581.
Deshpande, A. K. and Kung, H.-F. (1987). Mol. Cell. Biol. 7, 1285–1288.
LaBonne, C., Burke, B., and Whitman, M. (1995). Development 121, 1475–1486.
Mansour, S. J., Matten, W. T., Hermann, A. S., Candia, J. M., Rong, S., Fukasawa, K., Vande Woude, G. F., and Ahn, N. G. (1994). Science 265, 966–970.
Miura, K., Inouye, Y., Nakamori, H., Iwai, S., Ohtsuka, E., Ikehara, M., Noguchi, S., and Nishimura, S. (1986). Jpn. J. Cancer Res. (Gann) 77, 45–51.
Nassar, N., Horn, G., Herrmann, C., Scherer, A., McCormick, F., and Wittinghofer, A. (1995). Nature (London) 375, 554–560.
Pincus, M. R., Brandt-Rauf, P. W., Michl, J., and Friedman, F. K. (2000). Cancer Invest. 46, 35–53.
Ranginwale, M., Smith, S., Flom, J., Chie, L., Kanovsky, M., Chung, D., Friedman, F. K., Robinson, R. C., Brandt-Rauf, P. W., Yamaizumi, Z., Michl, J., and Pincus, M. R. (2001). Exp. Cell Res. 269, 162–169.
Reiss, Y., Goldstein, J. L., Seabra, M. C., Casey, P. J., and Brown, M. S. (1990). Cell 62, 81–88.
Stokoe, D., Macdonald S., Cadawallader, K., Symons, M., and Hancock, J. F. (1994). Science 264, 1463–1467.
White, M. A., Nicolette, C., Minden, A., Polverino, A., van Aelst, L., Karin, M., and Wigler, M. (1995). Cell 80, 533–541.
Xu, R.-H., Dong, Z., Maeno, M., Kim, J., Suzuki, A., Ueno, N., Sredni, D., Colburn, N. H., and Kung, H.-F. (1996). Proc. Natl. Acad. Sci. USA 93, 834–838.
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Chie, L., Friedman, F.K., Kung, HF. et al. Identification of the Site of Inhibition of Mitogenic Signaling by Oncogenic ras-p21 by a ras Effector Peptide. J Protein Chem 21, 367–370 (2002). https://doi.org/10.1023/A:1019998403181
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DOI: https://doi.org/10.1023/A:1019998403181