Skip to main content
Log in

Characterization of Salmonella spp. isolated from patients below 3 years old with acute diarrhoea

  • Published:
World Journal of Microbiology and Biotechnology Aims and scope Submit manuscript

Abstract

Fifteen strains of Salmonella were isolated from children with clinically diagnosed diarrhoea aged below 3 years old, who had been admitted to K7 ward, Pediatric Institute, Kuala Lumpur Hospital. The isolates were tested for their susceptibility to a range of antimicrobial agents, and typed by serological tests and randomly amplified polymorphic DNA (RAPD) fingerprinting. All the strains had a similar pattern of antimicrobial susceptibility, where they were susceptible to a wide range of antimicrobial agents. The serological test has typed them into three serovars, which were identified as Salmonella enterica ser. Akanji, Salmonella enterica ser. Hindmarch and Salmonella enterica ser. Richmond. In contrast, the RAPD fingerprinting classed them into two major clusters, cluster 1 consisting of 12 strains of Salmonella and cluster 2 consisting of three strains of Salmonella.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

References

  • Albert, M.J., Faruque, S.M., Neogi, P.K.B., Ansaruzzaman, M., Bhuyan, N.N., Alam, K. & Akbar, M.S. 1995 Controlled study of Esherichia coli diarrheal infections in Bangladeshi children. Journal of Clinical Microbiology 33, 973–977

    Google Scholar 

  • Anonymous. 2000 Salmonella serotypes recorded in the Public Health Laboratory Service Salmonella data set, April to June 2000. Communicable Disease Report 10, 332.

  • Bishop, W.P. & Ulshen, M.H. 1988 Bacterial gastroenteritis. Pediatric Clinics of North America 35, 69–87.

    Google Scholar 

  • Casalino, M., Yusuf, M.W., Nicoletti, M., Bazzicalupo, P., Coppo, A., Colonna, B., Cappelli, C., Bianchini, C., Falbo, V., Ahmed, H.J., Omar, K.H., Maxamuud, K.B. & Maimone, F. 1988 A two-year study of enteric infections associated with diarrhoeal diseases in children in urban Somalia. Transactions of the Royal Society of Tropical Medicine and Hygeine 82, 637–641.

    Google Scholar 

  • Cohen, M.B. & Laney, D.W. Jr., 1999 Infectious diarrhea. In Pediatric Gastrointestinal Disease Pathophysiology, Diagnosis, and Management, 2nd edn. eds. Wyllie, R., Hyams, J.S., pp. 348–370. Philadelphia: WB Saunders. ISBN 0-7216-7461-5.

    Google Scholar 

  • Conte, J.E. 2001 Manual of Antibiotics and Infectious Diseases. 9th edn. Lippincott Williams & Wilkins. ISBN 0-7817-2316-7.

  • Guerrant, R.L., Hughes, J.M., Lima, N.L. & Crane, J. 1990 Diarrhea in developed and developing countries: magnitude, special settings and etiologies. Reviews of Infectious Diseases 12(Suppl 1), S41.

    Google Scholar 

  • Hargett-Bean, N.T., Pavia, A.T. & Tauxen, R.T. 1988 Salomella isolates from humans in United States, 1984-1986. MMWR Morbility and Mortality Weekly Report. 3(SS2), 25–31.

    Google Scholar 

  • Herikstad, H., Hayes, P., Mokhtar, M., Fracaro, M.L., John Threlfall, E. & Angulo, F.J. 1997 Emerging quinolone-resistant Salmonella in the United States. Emerging Infectious Diseases 3, 371–372.

    Google Scholar 

  • Hoge, C.W., Gambel, J.M., Srijn, A., Pitarangsi, C. & Echeverria, P. 1998 Trends in antibiotic resistance among diarrhoeal pathogens isolated in Thailand over 15 years. Clinical Infectious Diseases 26, 341–345.

    Google Scholar 

  • Kunin, C.M. 1993 Resistance to antimicrobial drugs a worldwide calamity. Annals of Internal Medicine 118, 557–561.

    Google Scholar 

  • Malaysia Health 1996 Technical Report of the Director-General of Health, Malaysia. Tan Sri Dato (Dr) Abu Bakar Suleiman, p. 48. Malaysia: Ministry of Health.

    Google Scholar 

  • Murray, B.E., Alvarado, T., Kim, K.H., Vorachit, M., Jayanetra, P. & Levine, M.M. 1985 Increasing resistance to trimethoprim-sulfamethoxazole among isolates of Escherichia coli in developing countries. Journal of Infectious Diseases 147, 724–728.

    Google Scholar 

  • Nolan, L.K., Wooley, R.E., Brown, J. & Payeurk, J.B. 1991 Comparison of phenotypic characteristics of Salmonella spp. isolated from healthy and ill (infected) chickens. American Journal of Veterinary Research 52, 1512–1517.

    Google Scholar 

  • Powell, D.W. 1995 Approach to the patient with diarrhea. In Textbook of Gastroenterolgy, ed. Tadataka, Y. pp. 813–854. Philadelphia: JB Lippincott Company. ISBN 0-397-51491-3.

    Google Scholar 

  • Rahal, K., Wang, F., Schindler, J., Rowe, B., Cookson, B., Huovinen, P., et al. 1997 Reports on surveillance of antimicrobial resistance in individual countries. Clinical Infectious Diseases 24(Suppl 1), S169–175.

    Google Scholar 

  • Sack, R.B., Rahman, M., Yunus, M. & Khan, E.H. 1997 Antimicrobial resistance in organisms causing diarrhoeal disease. Clinical Infectious Diseases 24(Suppl 1), S102–105.

    Google Scholar 

  • Sambrook, J., Fritsch, E.F. & Maniatis, T. 1989 Molecular Cloning: A Laboratory Manual, 2nd edn. Cold Spring Harbor: Cold Spring Harbor Laboratory Press. ISBN 0879693738.

    Google Scholar 

  • Scuderi, G., Fantasia, M. & Niglio, T. 2000 Results of the three-year surveillance by the Italian SALM-NET system: human isolates of Salmonella serotypes. European Journal of Epidemiology 16, 377–383.

    Google Scholar 

  • Simanjuntak, C.H., Hardjining, S., Hasibuan, M.A., Pujarwoto & Koiman, I. 1998 Gastrointestinal infections in Southeast Asia (V). Technical Meeting of Counterparts on Seamic Data Exchange Program of Infectious Diseases. SEAMIC, Japan.

    Google Scholar 

  • Soto, A., Pitcher, D.G. & Soriano, F. 1991 A numerical analysis of ribosomal RNA gene patterns for typing clinical isolates of Corynebacterium group D2. Epidemiology and Infection 102, 263–272.

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

About this article

Cite this article

Shobirin, M.A., Shuhaimi, M., Abu-Bakar, F. et al. Characterization of Salmonella spp. isolated from patients below 3 years old with acute diarrhoea. World Journal of Microbiology and Biotechnology 19, 751–755 (2003). https://doi.org/10.1023/A:1025136830281

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1023/A:1025136830281

Navigation