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Novel male-specific molecular markers (MADC5, MADC6) in hemp

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Abstract

Decamer RAPD primers were tested on dioecious and monoecious hemp cultivars to identify sex-specific molecular markers. Two primers (OPD05 and UBC354) generated specific bands in male plants. These two DNA fragments were isolated, cloned and sequenced. Both markers proved to be unique, since no sequence with significant homology to OPD05961 and UBC354151 markers were found in databases. These markers were named MADC3 (OPD05961) and MADC4 (UBC354151) (Male-Associated DNA from Cannabis sativa). The markers were converted into sequence-characterized amplified region (SCAR) markers. The SCAR markers correlated with the sex of the segregating F2 population and proved the tight linkage to the male phenotype. Results of F2 plant population analysis suggest these markers are to be linked to the Y chromosome.

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Authors and Affiliations

  1. Dept. of Genetics & Plant Breeding, St. István University, Gödöllõ, Hungary

    Ottó Törjék, Nándor Bucherna, Erzsébet Kiss, Hajnalka Homoki & László E. Heszky

  2. Agricultural Research Institute, St. István University, Kompolt, Hungary

    Zsuzsanna Finta-Korpelová & Iván Bócsa

  3. Agricultural Biotechnology Center, Gödöllõ, Hungary

    István Nagy

Authors
  1. Ottó Törjék
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  2. Nándor Bucherna
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  3. Erzsébet Kiss
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  4. Hajnalka Homoki
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  5. Zsuzsanna Finta-Korpelová
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  6. Iván Bócsa
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  7. István Nagy
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  8. László E. Heszky
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Törjék, O., Bucherna, N., Kiss, E. et al. Novel male-specific molecular markers (MADC5, MADC6) in hemp. Euphytica 127, 209–218 (2002). https://doi.org/10.1023/A:1020204729122

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