Abstract
Retrotransposons constitute a ubiquitous and dynamic component of plant genomes. Intragenomic and intergenomic comparisons of related genomes offer potential insights into retrotransposon behavior and genomic effects. Here, we have used fluorescent in-situ hybridization to determine the chromosomal distributions of a Ty1-copia-like retrotransposon in the cotton AD-genome tetraploid Gossypium hirsutum and closely related putative A- and D-genome diploid ancestors. Retrotransposon clone A108 hybridized to all G. hirsutum chromosomes, approximately equal in intensity in the A- and D-subgenomes. Similar results were obtained by hybridization of A108 to the A-genome diploid G. arboreum, whereas no signal was detected on chromosomes of the D-genome diploid G. raimondii. The significance and potential causes of these observations are discussed.
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References
Beasley JO (1940) The origin of American tetraploid Gossypium species. Am Nat 74: 285–286.
Endrizzi JE, Turcotte EL, Kohel RJ (1985) Genetics, cytology, and evolution of Gossypium. Adv Genet 23: 271–375.
Flavell AJ, Dunbar E, Anderson R, Pearce SR, Hartley R, Kumar A (1992a) Ty1-copia group retrotransposons are ubiquitous and heterogeneous in higher plants. Nucleic Acids Res 20: 3639–3644.
Flavell AJ, Smith DB, Kumar A (1992b) Extreme heterogeneity of Ty1-copia group retrotransposons in plants. Mol Gen Genet 231: 233–242.
Fryxell PA (1992) A revised taxanomic interpretation of Gossypium L. Rheedea 2: 108–165.
Hanson RE, Islam-Faridi MN, Percival EA et al. (1996) Distributions of 5S and 18S-28S rDNA loci in a tetraploid cotton (Gossypium hirsutum L.) and its putative diploid ancestors. Chromosoma 105: 55–61.
Holland J, Spindler K, Horodyski F, Grabau E, Nichol S, VandePol S (1982) Rapid evolution of RNA genomes. Science 215: 1577–1585.
Jewell DC, Islam-Faridi MN (1994) Details of a technique for somatic chromosome preparation and C-banding of maize. In: Freeling M, Walbot V, eds. The Maize Handbook. New York: Springer-Verlag, pp 484–493.
Kamm A, Doudrick RL, Heslop-Harrison JS, Schmidt T (1996) The genomic and physical organization or Ty1-copia-like sequences as a component of large genomes in Pinus elliottii var. elliottii and other gymnosperms. Proc Natl Acad Sci USA 93: 2708–2713.
Katsiotis A, Schmidt T, Heslop-Harrison JS (1996) Chromosomal and genomic organization of Ty1-copia-like retrotransposon sequences in the genus Avena. Genome 39: 410–417.
Konieczny AD, Voytas DF, Cummings MP, Ausubel FM (1991) A superfamily of Arabidopsis thaliana retrotransposons. Genetics 127: 801–809.
Kumar A (1996) The adventures of the Ty1-copia group of retrotransposons in plants. Trends Genet 12: 41–43.
Masterson J (1994) Stomatal size in fossil plants: evidence for polyploidy in the majority of angiosperms. Science 264: 421–424.
Menzel MY (1954) A cytological method for genome analysis in Gossypium. Genetics 40: 214–223.
Pearce SR, Harrison G, Li D, Heslop-Harrison JS, Kumar A, Flavell AJ (1996a) The Ty1-copia group retrotransposons in Vicia species: copy number, sequence heterogeneity and chromosomal localisation. Mol Gen Genet 250: 305–315.
Pearce SR, Pich U, Harrison G et al. (1996b) The Ty1-copia group retrotransposons of Allium cepa are distributed throughout the chromosomes but are enriched in the terminal heterochromatin. Chromosome Res 4: 357–364.
Schmidt T, Kubis S, Heslop-Harrison JS (1995) Analysis and chromosomal localization of retrotransposons in sugar beet (Beta vulgaris L.)-LINEs and Ty1-copia-like elements as major components of the genome. Chromosome Res 3: 335–345.
Silhavy TJ, Berman ML, Enquist LW (1984) Experiments with Gene Fusion. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory Press, pp 144–146.
Soltis DE, Soltis PS (1993) Molecular data and the dynamic nature of polyploidy. Crit Rev Plant Sci 12: 243–273.
Suoniemi A, Anamthawat-Jonsson K, Arna T, Schulman A (1996) Retrotransposon BARE-1 is a major, dispersed component of the barley (Hordeum vulgare L.) genome. Plant Mol Biol 30: 1321–1329.
VanderWeil PL, Voytas DF, Wendel JF (1993) Ty1-copia-like retrotransposable element evolution in diploid and polyploid cotton (Gossypium L.). J Mol Evol 36: 429–447.
Voytas DF, Konieczny A, Cummings MP, Ausubel FM (1990) The structure, distribution and evolution of the Ta1 retrotransposable element family of Arabidopsis thaliana. Genetics 125: 713–721.
Wendel JF (1989) New World tetraploid cottons contain Old World cytoplasm. Proc Natl Acad Sci USA 86: 4132–4136.
Wendel JF, Albert VA (1992) Phylogenetics of the cotton genus (Gossypium L.): character-state weighted parsimony analysis of chloroplast DNA restriction site data and its systematic and biogeographic implications. Syst Bot 17: 115–143.
Woodruff RC, Slatko BE, Thompson JN Jr (1982) Factors affecting mutation rate in natural populations. In: Ashburner M, Carson HL, Thompson JN Jr, eds. The Genetics and Biology of Drosophila, Vol 3C, London and New York: Academic Press, Chapter 20, pp. 37–124.
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Hanson, R.E., Islam-Faridi, M.N., Crane, C.F. et al. Ty1-copia-retrotransposon Behavior in a Polyploid Cotton. Chromosome Res 8, 73–76 (2000). https://doi.org/10.1023/A:1009239522541
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DOI: https://doi.org/10.1023/A:1009239522541