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Newly developed liquid-based cytology. TACAS™: cytological appearance and HPV testing using liquid-based sample

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Abstract

Cell profiles determined by the thin-layer advanced cytology assay system (TACAS™), a liquid-based cytology technique newly developed in Japan, were analyzed in this study. Hybrid capture 2 (HC-2) was also performed using the liquid-based samples prepared by TACAS to ascertain its ability to detect human papillomavirus (HPV). Cell collection samples from uterine cervix were obtained from 359 patients and examined cytologically. A HC-2 assay for HPV was carried out in the cell specimens. All specimens were found to show background factors such as leukocytes. After excluding the 5 unsatisfactory cases from the total 354 cases, 82 cases (23.2%) were positive and 272 cases (76.8%) were negative for HPV. Cell specimens from 30 HPV-positive cases and 166 HPV-negative cases were subjected to 4 weeks of preservation at room temperature. Then, when subsequently re-assayed, 28 cases (93.3%) in the former group were found to be HPV positive and 164 cases (98.8%) in the latter group were found to be HPV negative. These results supported the excellent reproducibility of TACAS for HPV testing. A reasonable inference from the foregoing analysis is that TACAS may be distinguished from other liquid-based cytological approaches, such as ThinPrep and SurePath, in that it can retain the cell backgrounds. Furthermore, this study raises the possibility that cell specimens prepared using TACAS could be preserved for at least 4 weeks prior to carrying out a HC-2 assay for HPV.

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References

  1. http://apps.who.int/hpvcentre/statistics/dynamic/ico/SummaryReportsSelect.cfm. Accessed 2 Dec 2010.

  2. Konno R, Dobbelaere KO, Godeaux OO, et al. Immunogenicity, reactogenicity, and safety of human papillomavirus 16/18 AS04-adjuvanted vaccine in Japanese women: interim analysis of a phase II, double-blind, randomized controlled trial at month 7. Int J Gynecol Cancer. 2009;19:905–11.

    Article  PubMed  Google Scholar 

  3. Doyle B, O’Farrell C, Mahoney E, et al. Liquid-based cytology improves productivity in cervical cytology screening. Cytopathology. 2006;17:60–4.

    Article  PubMed  CAS  Google Scholar 

  4. Kirschner B, Simonsen K, Junge J. Comparison of conventional Papanicolaou smear and SurePath liquid-based cytology in the Copenhagen population screening programme for cervical cancer. Cytopathology. 2006;17:187–94.

    Article  PubMed  CAS  Google Scholar 

  5. Williams AR. Liquid-based cytology and conventional smears compared over two 12-month periods. Cytopathology. 2006;17:82–5.

    Article  PubMed  CAS  Google Scholar 

  6. Ronco G, Cuzick J, Pierotti P, et al. Accuracy of liquid based versus conventional cytology: overall results of new technologies for cervical cancer screening: randomised controlled trial. BMJ. 2007;335:28.

    Article  PubMed  Google Scholar 

  7. Strander B, Andersson-Ellstrom A, Milsom I, et al. Liquid-based cytology versus conventional Papanicolaou smear in an organized screening program: a prospective randomized study. Cancer. 2007;111:285–91.

    Article  PubMed  Google Scholar 

  8. Bergeron C, Bishop J, Lemarie A, et al. Accuracy of thin-layer cytology in patients undergoing cervical cone biopsy. Acta Cytol. 2001;45:519–24.

    Article  PubMed  CAS  Google Scholar 

  9. Hessling JJ, Raso DS, Schiffer B, et al. Effectiveness of thin-layer preparations vs. conventional Pap smears in a blinded, split-sample study. Extended cytologic evaluation. J Reprod Med. 2001;46:880–6.

    PubMed  CAS  Google Scholar 

  10. Schledermann D, Ejersbo D, Hoelund B. Improvement of diagnostic accuracy and screening conditions with liquid-based cytology. Diagn Cytopathol. 2006;34:780–5.

    Article  PubMed  Google Scholar 

  11. Ferenczy A, Robitaille J, Franco E, et al. Conventional cervical cytologic smears vs. ThinPrep smears. A paired comparison study on cervical cytology. Acta Cytol. 1996;40:1136–42.

    PubMed  CAS  Google Scholar 

  12. Sherman ME, Mendoza M, Lee KR, et al. Performance of liquid-based, thin-layer cervical cytology: correlation with reference diagnoses and human papillomavirus testing. Mod Pathol. 1998;11:837–43.

    PubMed  CAS  Google Scholar 

  13. Arbyn M, Bergeron C, Klinkhamer P, et al. Liquid compared with conventional cervical cytology: a systematic review and meta-analysis. Obstet Gynecol. 2008;111:167–77.

    Article  PubMed  Google Scholar 

  14. Davey E, Barratt A, Irwig L, et al. Effect of study design and quality on unsatisfactory rates, cytology classifications, and accuracy in liquid-based versus conventional cervical cytology: a systematic review. Lancet. 2006;367:122–32.

    Article  PubMed  Google Scholar 

  15. Siebers AG, Klinkhamer PJ, Grefte JM, et al. Comparison of liquid-based cytology with conventional cytology for detection of cervical cancer precursors: a randomized controlled trial. JAMA. 2009;302:1757–64.

    Article  PubMed  CAS  Google Scholar 

  16. Masumoto N, Fujii T, Ishikawa M, et al. Papanicolaou tests and molecular analyses using new fluid-based specimen collection technology in 3000 Japanese women. Br J Cancer. 2003;88:1883–8.

    Article  PubMed  CAS  Google Scholar 

  17. Taoka H, Yamamoto Y, Sakurai N, et al. Comparison of conventional and liquid-based cytology, and human papillomavirus testing using SurePath preparation in Japan. Hum Cell. 2010;23:126–33.

    Article  PubMed  Google Scholar 

  18. Jamison J, Wilson RT, Carson J. The evaluation of human papillomavirus genotyping in cervical liquid-based cytology specimens; using the Roche Linear Array HPV genotyping assay. Cytopathology. 2009;20:242–8.

    Article  PubMed  CAS  Google Scholar 

  19. Lee JK, Kim MK, Song SH, et al. Comparison of human papillomavirus detection and typing by hybrid capture 2, linear array, DNA chip, and cycle sequencing in cervical swab samples. Int J Gynecol Cancer. 2009;19:266–72.

    Article  PubMed  Google Scholar 

  20. Hardie A, Moore C, Patnick J, et al. High-risk HPV detection in specimens collected in SurePath preservative fluid: comparison of ambient and refrigerated storage. Cytopathology. 2009;20:235–41.

    Article  PubMed  CAS  Google Scholar 

  21. Obwegeser JH, Brack S. Does liquid-based technology really improve detection of cervical neoplasia? A prospective, randomized trial comparing the ThinPrep Pap Test with the conventional Pap Test, including follow-up of HSIL cases. Acta Cytol. 2001;45:709–14.

    Article  PubMed  CAS  Google Scholar 

  22. Taylor S, Kuhn L, Dupree W, et al. Direct comparison of liquid-based and conventional cytology in a South African screening trial. Int J Cancer. 2006;118:957–62.

    Article  PubMed  CAS  Google Scholar 

  23. Sykes PH, Harker DY, Miller A, et al. A randomised comparison of SurePath liquid-based cytology and conventional smear cytology in a colposcopy clinic setting. BJOG. 2008;115:1375–81.

    Article  PubMed  CAS  Google Scholar 

  24. Beerman H, van Dorst EB, Kuenen-Boumeester V, et al. Superior performance of liquid-based versus conventional cytology in a population-based cervical cancer screening program. Gynecol Oncol. 2009;112:572–6.

    Article  PubMed  CAS  Google Scholar 

  25. Davey E, d’Assuncao J, Irwig L, et al. Accuracy of reading liquid based cytology slides using the ThinPrep Imager compared with conventional cytology: prospective study. BMJ. 2007;335:31.

    Article  PubMed  Google Scholar 

  26. Sweeney BJ, Haq Z, Happel JF, Weinstein B, Schneider D. Comparison of the effectiveness of two liquid-based Papanicolaou systems in the handling of adverse limiting factors, such as excessive blood. Cancer. 2006;108:27–31.

    Article  PubMed  Google Scholar 

  27. Wright TC Jr. Cervical cancer screening in the 21st century: is it time to retire the PAP smear? Clin Obstet Gynecol. 2007;50:313–23.

    Article  PubMed  Google Scholar 

  28. Kjaer S, Hogdall E, Frederiksen K, et al. The absolute risk of cervical abnormalities in high-risk human papillomavirus-positive, cytologically normal women over a 10-year period. Cancer Res. 2006;66:10630–6.

    Article  PubMed  CAS  Google Scholar 

  29. Khan MJ, Castle PE, Lorincz AT, et al. The elevated 10-year risk of cervical precancer and cancer in women with human papillomavirus (HPV) type 16 or 18 and the possible utility of type-specific HPV testing in clinical practice. J Natl Cancer Inst. 2005;97:1072–9.

    Article  PubMed  Google Scholar 

  30. Castle PE, Rodríguez AC, Burk RD, et al. Short term persistence of human papillomavirus and risk of cervical precancer and cancer: population based cohort study. BMJ. 2009;339:b2569. doi:10.1136/bmj.b2569.

  31. http://www.asccp.org/pdfs/consensus/hpv_genotyping_20090320.pdf. Accessed 5 Dec 2010.

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Acknowledgments

We thank Dr Toshiaki Oharaseki for pathological discussion, and the cytotechnologists, Mr. Katsuji Taguchi, Mr. Masashi Fujita, Ms. Yoshie Muraishi, and Ms. Tomoko Kawabata for cytoscreening. We also thank Ms. Miyuki Saito for her helpful discussion during this study.

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Authors and Affiliations

  1. Department of Obstetrics and Gynecology, Toho University School of Medicine, Ohashi Medical Center, 2-17-6 Ohashi, Meguro-ku, Tokyo, 153-8515, Japan

    Kaneyuki Kubushiro, Hideki Taoka, Nobuyuki Sakurai, Yasuhiro Yamamoto, Akiko Kurasaki & Yasuyuki Asakawa

  2. Department of Surgical Pathology, Toho University School of Medicine, Ohashi Medical Center, Tokyo, Japan

    Minoru Iwahara & Kei Takahashi

Authors
  1. Kaneyuki Kubushiro
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  2. Hideki Taoka
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  3. Nobuyuki Sakurai
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  5. Akiko Kurasaki
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  6. Yasuyuki Asakawa
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  7. Minoru Iwahara
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Correspondence to Kaneyuki Kubushiro.

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Kubushiro, K., Taoka, H., Sakurai, N. et al. Newly developed liquid-based cytology. TACAS™: cytological appearance and HPV testing using liquid-based sample. Human Cell 24, 115–120 (2011). https://doi.org/10.1007/s13577-011-0020-5

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  • DOI: https://doi.org/10.1007/s13577-011-0020-5

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