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Antioxidant activity of polyphenolic extract of Phyllanthus emblica against lead acetate induced oxidative stress

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Abstract

Phyllanthus emblica (Euphorbiaceae) possesses a vast ethnomedical history and represents a phytochemical reservoir of heuristic medicinal value. Polyphenolic extract of P. emblica fruits, was investigated for antioxidant potential against lead acetate induced toxicity in female Wistar rats. The liver of female Wistar rats treated with lead acetate at different concentrations showed significant increase in lipid peroxidation and decrease in activities of superoxide dismutase and catalase. Furthermore, lead acetate also caused decrease in reduced glutathione content in rat liver. However, the co-incubation of extract and lead acetate showed significant protection in concentration-dependent manner. Furthermore, the polyphenolic extract of P. emblica fruits also found to have potent free radical scavenging activity as assessed by reducing power assay, 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical (IC50 15.5 μg/mL), superoxide radical (IC50 26 μg/mL) and hydrogen peroxide scavenging assays (IC50 22 μg/mL) under in vitro conditions. Moreover the phytochemical characterization of the extract was also measured by determining total phenolic, flavonoids and tannin contents. This activity of the extract may be due to the total polyphenolic contents present in it. The present study concluded that a polyphenolic rich fraction of P. emblica fruits is a potential source of natural antioxidants and thus could prevent oxidative stress induced by heavy metal toxicants.

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  1. Department of Zoology, University School of Sciences, Gujarat University, Ahmedabad, 380009, India

    Sarmistha Saha & Ramtej J. Verma

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Saha, S., Verma, R.J. Antioxidant activity of polyphenolic extract of Phyllanthus emblica against lead acetate induced oxidative stress. Toxicol. Environ. Health Sci. 7, 82–90 (2015). https://doi.org/10.1007/s13530-015-0224-2

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