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Phylogenetic and systematic study of Korean Orius species (Heteroptera: Anthocoridae) on the basis of molecular and morphological data

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Abstract

A phylogenetic and systematic study of Orius species (Heteroptera: Anthocoridae) from Korea has been conducted using both morphological and molecular characters. Thirty morphological character states were coded for 10 strains of 9 species. Five molecular markers, partial cytochrome c oxidase I (COI), cytochrome b (CytB), 16S rRNA (16S), 18S rRNA (18S), and 28S rRNA (28S), from mitochondrial and nuclear genes, were tested. Phylogenetic analyses based on molecular data were conducted by minimum evolution, maximum parsimony, maximum likelihood, and Bayesian phylogenetic (BP) analyses. Analysis of morphological data was performed using the parsimony programs NONA, and the combined dataset of morphological and molecular data was analyzed using BP analyses. The results of this study indicate that use of COI and CytB enabled relatively effective identification of species, whereas the sequences of 16S, 18S and 28S did not enable identification of closely related species such as Orius minutus and O. strigicollis. We discuss the usefulness of the five molecular markers for determining phylogenetic relationships and identifying the species.

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Acknowledgments

The authors thank Dr Hyojoong Kim, Dr Kazutaka Yamada, Dr Tomohide Yasuanga, Dr Tadashi Ishikawa, Dr Randall T. Schuh, Dr Kim A. Hoelmer, Mr Sangwook Park, Mr Gary Pixsely, and Ms Ram K. Duwal for their assistance with sampling and for valuable discussion. This research was supported by a grant from the Agenda Project (grant no. 102966290) and Biogreen 21 Program (grant no. 034032), by the Rural Development Administration in the Republic of Korea.

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  1. Laboratory of Insect Biosystematics, Division of Entomology, Research Institute for Agricultural and Life Sciences, Department of Agricultural Biotechnology, Seoul National University, San 56-1 Shilim-dong, Gwanak-gu, Seoul, 151-742, Korea

    Sunghoon Jung & Seunghwan Lee

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  1. Sunghoon Jung
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  2. Seunghwan Lee
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Correspondence to Seunghwan Lee.

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Jung, S., Lee, S. Phylogenetic and systematic study of Korean Orius species (Heteroptera: Anthocoridae) on the basis of molecular and morphological data. Appl Entomol Zool 46, 153–164 (2011). https://doi.org/10.1007/s13355-011-0024-3

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