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Identification of genetic variations of cultivated and weedy types of Perilla species in Korea and Japan using morphological and SSR markers

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Abstract

To better understand the genetic diversity and relationships of the two cultivated types of Perilla crop and their weedy types in Korea and Japan, we evaluated the genetic variations of 56 accessions by assessing five morphological characteristics and 18 SSR markers. The two cultivated types of var. frutescens and var. crispa were clearly distinguished by seed size, whereas most accessions of cultivated and weedy types of var. crispa cannot be distinguished strictly by seed characteristics. A total of 165 alleles with the SSR analysis were detected with an average number of 9.2 alleles per locus among the 56 Perilla accessions. The number of alleles per locus ranged from two for KWPE-56 and KWPE-39 to 21 for GBPFM-204. Additionally, the genetic diversity of each locus ranged from 0.497 at KWPE-56 and KWPE-39 to 0.959 at GBPFM-204, with an average of 0.692. The average genetic diversity values were 0.549, 0.685, 0.451 and 0.557 for cultivated and weedy types of var. frutescens and for cultivated and weedy types of var. crispa, respectively. The weedy type accessions of var. frutescens and var. crispa evidenced greater variation than the corresponding cultivated type accessions. The accessions of the cultivated and weedy types of var. frutescens and var. crispa from Korea exhibited greater SSR diversity than those of Japan. An UPGMA phylogenetic tree revealed three major groups, which was congruent with their morphological characteristics except for a few odd accessions. SSR markers clarified the genetic relationships between var. frutescens and var. crispa and helped improve our understanding of the genetic diversity of the two cultivated types of P. frutescens and their weedy types in Korea and Japan.

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Acknowledgments

This study was supported by Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education, Science and Technology (2012R1A1A4A01001247), and a Korea Research Foundation Grant funded by the Next-Generation BioGreen 21 Program (Plant Molecular Breeding Center, No. PJ008018012012).

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Authors and Affiliations

  1. Department of Applied Plant Sciences, College of Agriculture and Life Science, Kangwon National University, Chuncheon, 200-701, Korea

    Kyu Jin Sa, Seung Hun Choi & Ju Kyong Lee

  2. Plant Germ-Plasm Institute Faculty of Agriculture, Kyoto University, Nakajo 1, Mozume-cho, Muko, Kyoto, 617-001, Japan

    Mariko Ueno

  3. Department of Molecular Biosciences, Kangwon National University, Chuncheon, 200-701, Korea

    Kyong-Cheul Park

  4. Department of Plant Sciences, Kongju National University, Yesan, Kongju, 340-702, Korea

    Young Jin Park

  5. National Academy of Agricultural Science, Rural Development Administration, Suwon, 441-707, Korea

    Kyoung Ho Ma

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  1. Kyu Jin Sa
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  2. Seung Hun Choi
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  3. Mariko Ueno
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  4. Kyong-Cheul Park
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  5. Young Jin Park
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  6. Kyoung Ho Ma
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  7. Ju Kyong Lee
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Correspondence to Ju Kyong Lee.

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Sa, K.J., Choi, S.H., Ueno, M. et al. Identification of genetic variations of cultivated and weedy types of Perilla species in Korea and Japan using morphological and SSR markers. Genes Genom 35, 649–659 (2013). https://doi.org/10.1007/s13258-013-0117-1

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  • DOI: https://doi.org/10.1007/s13258-013-0117-1

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