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Development, characterization, and inheritance of 113 novel EST-SSR markers in the Pacific oyster (Crassostrea gigas)

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Abstract

A total of 113 novel EST-derived simple sequence repeat (EST-SSR) markers were developed in the Pacific oyster (Crassotrea gigas). Polymorphisms of these markers were evaluated in a wild population of 30 individuals. The number of alleles per locus ranged from 2 to 27 with an average of 6.3, and the observed and expected heterozygosities varied from 0 to 0.9667 and from 0.0333 to 0.9701, respectively. Mendelian segregations were tested for 24 of the markers that were polymorphic in one family produced by single-pair mating. Null alleles were discovered at four loci. Nine tests of segregation ratios revealed significant departures from expected Mendelian ratios. As a useful addition to the collection of the microsatellites that are now available for C. gigas, these EST-SSR markers will help the advance in investigation of QTL mapping and genetic diversity in this species.

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Authors and Affiliations

  1. Key Laboratory of Mariculture Ministry of Education, Ocean University of China, Qingdao, 266003, China

    Qi Li, Qingzhi Wang, Mingjun Qi, Jianlong Ge & Rihao Cong

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  1. Qi Li
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  2. Qingzhi Wang
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  3. Mingjun Qi
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  4. Jianlong Ge
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  5. Rihao Cong
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Correspondence to Qi Li.

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Li, Q., Wang, Q., Qi, M. et al. Development, characterization, and inheritance of 113 novel EST-SSR markers in the Pacific oyster (Crassostrea gigas). Genes Genom 33, 313–316 (2011). https://doi.org/10.1007/s13258-011-0021-5

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  • DOI: https://doi.org/10.1007/s13258-011-0021-5

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