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Evaluation of the genetic diversity and population structure of sesame (Sesamum indicum L.) using microsatellite markers

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Abstract

Sixteen polymorphic microsatellite (SSR) markers, developed from an SSR-enriched genomic DNA library of sesame (Sesamum indicum L.), were used to assess genetic diversity, phylogenetic relationships, and population structure among 150 sesame accessions collected from 22 countries. A total of 121 alleles were detected among the sesame accessions. The number of detected alleles varied from 2 to 18, with an average of 7.6 alleles per locus. Polymorphism information content values ranged from 0.03 to 0.79, with an average of 0.42. These values indicated an excess of heterozygous individuals at 16 loci and an excess of homozygous individuals at three loci. Of these, 32 genotype-specific alleles were identified at 11 of 16 polymorphic SSR markers. Cluster analyses were performed by accession and population, revealing a complex accession distribution pattern with mean genetic similarity coefficient of 0.45 by accession and 0.52 by population. The wide variation in genetic similarity among the accessions revealed by SSRs reflected a high level of polymorphism at the DNA level. Model-based structure analysis revealed the presence of three groups that were basically consistent with the clustering results based on genetic distance. These findings may be used to augment the sesame germplasm and to increase the effectiveness of sesame breeding.

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Authors and Affiliations

  1. Department of Plant Resources, College of Industrial Sciences, Kongju National University, Yesan, 340-702, Korea

    Young-Il Cho, Won-Hee Ra, Jong-Wook Chung & Yong-Jin Park

  2. Bio-resources Management Division, Research Policy Bureau, Rural Development Administration, Suwon, 441-707, Korea

    Jong-Hyun Park

  3. Department of Crop Science, Chungbuk National University, Cheongju, 361-763, Korea

    Chul-Won Lee

  4. National Agrobiodiversity Center, National Institute of Agricultural Biotechnology, Rural Development Administration, Suwon, 441-707, Korea

    Jong-Wook Chung, Jung-Ro Lee, Kyung-Ho Ma, Seok-Young Lee, Kang-Seob Lee & Myung-Chul Lee

  5. Legume Bio-Resource Center of Green Manure, Kongju National University, Yesan, 340-702, Korea

    Yong-Jin Park

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  1. Young-Il Cho
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  2. Jong-Hyun Park
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Correspondence to Yong-Jin Park.

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Cho, YI., Park, JH., Lee, CW. et al. Evaluation of the genetic diversity and population structure of sesame (Sesamum indicum L.) using microsatellite markers. Genes Genom 33, 187–195 (2011). https://doi.org/10.1007/s13258-010-0130-6

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  • DOI: https://doi.org/10.1007/s13258-010-0130-6

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