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Development and characterization of twenty-five new polymorphic microsatellite markers in proso millet (Panicum miliaceum L.)

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Abstract

Millets such as proso millet have excellent nutritional properties and could become a basic resource for crop breeding programs and food diversification. In this study, 25 polymorphic microsatellite markers were developed and characterized through construction of an SSR-enriched library from genomic DNA of proso millet (Panicum miliaceum L.). In total, 110 alleles were detected, with an average of 4.4 alleles per locus. Values of major allele frequency (M AF ) and expected heterozygosity (H E ) ranged from 0.36 to 0.98 (mean = 0.73) and from 0.04 to 0.74 (mean = 0.37), respectively. The mean genetic similarity coefficient was 0.3711, indicating that among 50 accessions of proso millet there was wide genetic variation. The newly developed microsatellite markers should be useful tools for assessing genetic diversity, understanding population structure, and breeding of proso millet.

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Authors and Affiliations

  1. Department of Plant Resources, College of Industrial Science, Kongju National University, Yesan, 340-702, Korea

    Young-Il Cho, Jong-Wook Chung & Yong-Jin Park

  2. National Agrobiodiversity Center, National Institute of Agricultural Biotechnology, RDA, Seodun-Dong, Suwon, 441-100, Korea

    Gi-An Lee, Kyung-Ho Ma & Jae-Gyun Gwag

  3. Rice DNA and Quality Testing Laboratory, Basmati Export Development Foundation, SVBP University of Agriculture and Technology, Meerut, 250110, India

    Anupam Dixit

Authors
  1. Young-Il Cho
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  2. Jong-Wook Chung
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  3. Gi-An Lee
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  4. Kyung-Ho Ma
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  5. Anupam Dixit
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  6. Jae-Gyun Gwag
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  7. Yong-Jin Park
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Correspondence to Yong-Jin Park.

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Cho, YI., Chung, JW., Lee, GA. et al. Development and characterization of twenty-five new polymorphic microsatellite markers in proso millet (Panicum miliaceum L.). Genes Genom 32, 267–273 (2010). https://doi.org/10.1007/s13258-010-0007-8

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  • DOI: https://doi.org/10.1007/s13258-010-0007-8

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