Abstract
A method was standardized to isolate quality DNA from cattle and buffalo fat for species identification using QIAamp DNA stool mini kit. The quality of the DNA was sufficient enough to amplify universal primers viz., mt 12S rRNA and mt 16S rRNA, and species specific D loop primers for cattle and buffalo. The sensitivity of the PCR assay in the species specific D loop primer amplification was with a detection level of 0. 47 ng cattle DNA and 0.23 ng buffalo DNA in simplex and, 0. 47 ng cattle DNA and 0.12 ng buffalo DNA in duplex PCR. It is a potentially reliable method for DNA detection to authenticate animal fat.
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The authors thank the National Research on Meat for providing the necessary facilities to carry out this study.
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Highlights
• A method standardized to isolate DNA from cattle and buffalo fat for PCR assay.
• The quality of DNA was sufficient enough to amplify universal primers and species specific primers, and to quantitatively identify species of fat
• The method and assay may be used by food inspection departments as well as manufacturers
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Vaithiyanathan, S., Kulkarni, V.V. Species identification of cattle and buffalo fat through PCR assay. J Food Sci Technol 53, 2077–2082 (2016). https://doi.org/10.1007/s13197-016-2198-8
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DOI: https://doi.org/10.1007/s13197-016-2198-8