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Protective effect of black raspberry seed containing anthocyanins against oxidative damage to DNA, protein, and lipid

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Abstract

This study aimed to determine bioactive components and radical scavenging capacity of black raspberry seed extracts as byproducts obtaining during the juice (FSE) and wine (WSE) making process. Cyanidin-3-O-rutinoside was identified as a major anthocyanin and the total anthocyanin contents of fresh and wine seed were 78.24 and 41.61 mg/100 g of dry weight, respectively. The total phenolic and flavonoid contents of FSE and WSE were 2.31 g gallic acid equivalent (GAE) and 360.95 mg catechin equivalent (CE), and 2.44 g GAE and 379.54 mg CE per 100 g dry weight, respectively. The oxygen radical absorbance capacity (ORAC) values were 1041.9 μM TE/g for FSE and 1060.4 μM TE/g for WSE. Pretreatment of the FSE and WSE inhibited the generation of intracellular reactive oxygen species (ROS), DNA and protein damage induced by hydroxyl radicals, and Fe3+/ascorbic acid-induced lipid peroxidation in a dose dependent manner. WSE more effectively protected from oxidative damage than FSE. Results from the current study suggest that black raspberry seeds as byproducts from juice and wine processing could be potential sources for natural antioxidants.

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Acknowledgments

This work was supported by the Priority Research Centers Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education, Science and Technology (2009-0094017).

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Authors and Affiliations

  1. Department of Food and Nutrition, Duksung Women’s University, 419 Ssangmun-dong, Tobong-gu, Seoul, 132-714, South Korea

    Mi-Hee Choi & Gun-Hee Kim

  2. Department of Food Science and Technology, Sejong University, 98 Gunja-dong, Kwangjin-gu, Seoul, 134-747, South Korea

    Mi-Hee Choi & Soon-Mi Shim

Authors
  1. Mi-Hee Choi
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  2. Soon-Mi Shim
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  3. Gun-Hee Kim
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Corresponding authors

Correspondence to Soon-Mi Shim or Gun-Hee Kim.

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Research highlights

• Black raspberry seeds are produced during juice and winemaking procedure.

• Total phenolic content and flavonoids in seed extracts were determined.

• Pretreatment of seed extracts inhibited the generation of reactive oxygen species.

• Seed extracts protected DNA, protein, and lipid against cellular oxidative damage.

• Seeds as byproducts could have a potential as natural antioxidants.

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Choi, MH., Shim, SM. & Kim, GH. Protective effect of black raspberry seed containing anthocyanins against oxidative damage to DNA, protein, and lipid. J Food Sci Technol 53, 1214–1221 (2016). https://doi.org/10.1007/s13197-015-2094-7

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  • DOI: https://doi.org/10.1007/s13197-015-2094-7

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