Abstract
The goral (Naemorhedus caudatus), a species of the Caprinae, is classified as globally vulnerable on the IUCN Red List of Threatened Species because of its sharply declining population size, which is attributable to habitat destruction. In this study, we determined and described the complete mitochondrial genome of N. c. griseus, a goral subspecies from the western Sichuan of China; the mitochondrial genome of this goral is 16,557 bp in length (GenBank accession No: MG865962) and contains 13 protein-coding genes (PCGs), two rRNA genes, 23 tRNA genes, and one control region (D-loop). Ten PCGs start with ATG, but ND2, ND3, and ND5 use ATA as the start codon. The overall base composition of the mitochondrial DNA is 34.6% A, 28.6% T, 22.4% C, and 14.4% G. The G+C content is 36.8%. Phylogenetic analysis revealed that N. c. griseus clustered into a clade with other Naemorhedus species with high bootstrap support. This work provides additional molecular information for studying the conservation genetics and evolutionary relationships of this endangered species.
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References
Boore JL (1999) Animal mitochondrial genomes. Nucleic Acids Res 27(8):1767–1780
Conant GC, Wolfe KH (2008) GenomeVx: simple web-based creation of editable circular chromosome maps. Bioinformatics 24(6):861–862
De Giorgi C, Saccone C (1989) Mitochondrial genome in animal cells. Cell Biophys 14(1):67–78
Duckworth JW, MacKinnon J, Tsytsulina K (2008) Naemorhedus caudatus. The IUCN Red List of Threatened Species 2008: e.T14295A4429742. Downloaded on 13 February 2018
Laslett D, Canback B (2008) ARWEN, a program to detect tRNA genes in metazoan mitochondrial nucleotide sequences. Bioinformatics 24(2):172–175
Liu Y, Jiang L (2017) The complete mitochondrial genome sequence of the Himalayan goral, Naemorhedus goral (Cetartiodactyla: Caprinae). Mitochondrial DNA 28(2):233–235
Ren ZM, Wang Y, Zhu J, Su X (2017) Complete mitochondrial genome of Naemorhedus goral (Caprinae), a threatened species from the Himalayan and Hindu Kush regions. Conserv Genet Resour. https://doi.org/10.1007/s12686-017-0947-2
Sambrook J, Russell DW (2001) Molecular cloning: a laboratory manual, 3rd edn. Cold Spring Harbor Laboratory Press, New York
Shi R, Sun Y, Teng L, Liu Z (2016) Complete mitochondrial genome of red goral (Naemorhedus baileyi) and a complete estimate of the phylogenetic relationships in Caprinae. Mitochondrial DNA 27(4):2721–2722
Wang S (1998) China Endangered Species Red List (Mammalia). Science Press, Beijing
Wang YX (2003) A complete checklist of mammal species and subspecies in China. China Forestry Publishing House, Beijing
Zhang B, Pan T, Ren L, Wang H, Hu C (2015) Mitochondrial genome of the Naemorhedus griseus (Bovidae: Naemorhedus). Mitochondrial DNA 26(4):636–637
Acknowledgements
This work was supported by grants from the National Natural Science Foundation of China (31370407). We thank Pu Zhao, Qian Su, Xue Liu, Yuhan Wu, and Mengmeng Dong for their assistance in experiments and data analysis.
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Yao, Y., Li, D., Xie, M. et al. The complete mitochondrial genome and phylogeny of a wild goral from the western Sichuan of China (Naemorhedus caudatus griseus). Conservation Genet Resour 11, 385–388 (2019). https://doi.org/10.1007/s12686-018-1024-1
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DOI: https://doi.org/10.1007/s12686-018-1024-1