Abstract
We developed and characterized 13 microsatellite markers for Nuphar submersa, a critically endangered freshwater macrophyte of central Japan. In all the studied populations, ten out of isolated 13 loci showed polymorphism. The 13 loci showed one to five alleles per locus, with observed and expected heterozygosities ranging from 0 to 0.40 and from 0 to 0.67, respectively. Between 5 and 11 of the 13 loci were successfully amplified across the six other Nuphar taxa. The markers described here will be useful for a conservation genetic study of the species and informing the development of effective conservation programs of N. submersa and related species.
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Acknowledgments
We thank Y. Tsukasaki, M. Mori, Y. Shibata, S. Shibata, Y. Komine, and Y. Sugita for their helpful support of sampling. We also thank J. Worth for his helpful comments on this manuscript. This work was supported by the Global Environment Research Fund (D-0903) of the Ministry of the Environment, Japan and Grants in Aid for Scientific Research (20770074) from the Japan Society for the Promotion of Science.
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Yokogawa, M., Shiga, T., Kaneko, S. et al. Development of nuclear microsatellite markers for the critically endangered freshwater macrophyte, Nuphar submersa (Nymphaeaceae), and cross-species amplification in six additional Nuphar taxa. Conservation Genet Resour 4, 295–298 (2012). https://doi.org/10.1007/s12686-011-9528-y
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DOI: https://doi.org/10.1007/s12686-011-9528-y