Abstract
Congenital toxoplasmosis can lead to severe damage for the fetus and newborn. Considering that the seroepidemiology of Toxoplasma infection in the pregnant women is poorly studied in west of Iran, the main objective of this study was to estimate the seroprevalence and potential risk factors for congenital toxoplasmosis in Delfan, Iran. In this cross-sectional study, the serum samples obtained from pregnant women who were referred to health centers for routine monitoring of the pregnancy. Totally, 264 sera were screened for IgG and IgM anti-T. gondii antibodies by enzyme linked immunosorbent assay method. All women with IgM anti-T. gondii positive checked by RT-PCR and confirmed. In addition, structured questionnaires were used to obtain information on risk factors for T. gondii infection. Anti-Toxoplasma IgG and IgM were positive in 66 (25 %) and 15 (5.7 %) respectively. Seropositive subjects were more frequently seen in those with >30 years old compared to younger women (<25 years old) (p < 0.001). No significant relationship was found between the seroprevalence of T. gondii infection and level of education, and gestational age (p > 0.05), while there was statistical difference between the infection with cat exposure, consumption of raw/undercooked meat, eating raw or uncooked eggs, consumption of unwashed vegetables and drinking unpasteurized milk (p < 0.001). In the present study, it was found that T. gondii infection was present among pregnant women in west of Iran. Therefore, it is suggested to provide health education for preventing primary infection during pregnancy and subsequently congenital toxoplasmosis in the pregnant women.
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Abdi J, Shojaee S, Mirzaee A, Keshavarz H (2008) Seroprevalence of toxoplasmosis in pregnant women in Ilam province, Iran. Iran J Parasitol 3:34–37
Al-Harthi SA, Jamjoom MB, Ghazi HO (2006) Seroprevalence of Toxoplasma gondii among pregnant women in Makkah, Saudi Arabia. Umm Al-Qura Univ J Sci Med Eng 18:217–227
Al-Mendalawi MD (2010) The investigation of congenital toxoplasmosis in a tertiary care hospital in Turkey. Saudi Med J 31:96
Alsammani M (2014) Sero-epidemiology and risk factors for Toxoplasma gondii among pregnant women in Arab and African countries. J Parasit Dis. doi:10.1007/s12639-014-0558-8
Cheraghipour K, Taherkhani H, Fallah Mohammad SA, Sardarian K, Rostamnezhad M, Maghsoudi A (2010) Seroprevalence of toxoplasmosis in pregnant women admitted to the health centers of Khorram-Abad City, Iran. Sci J Hamadan Univ Med Sci Health Serv 17:46–51
Daryani A, Sagha M (2004) Seroepidemiology of toxoplasmosis in women referred to medical health laboratory before marriage, Ardebil. J Ardabil Univ Med Sci 4:19–25
Daryani A, Sarvi S, Aarabi M et al (2014) Seroprevalence of Toxoplasma gondii in the Iranian general population: a systematic review and meta-analysis. Acta Trop 137:185–194
Dubey J (2009) Toxoplasmosis in sheep-the last 20 years. Vet Parasitol 163:1–14
Ebrahimzadeh A, Mohammadi S, Salimi-Khorashad A, Jamshidi A (2013) Seroprevalence of toxoplasmosis among pregnant women referring to the reference Laboratory of Zahedan, Iran. Zahedan J Res Med Sci 15:32–35
Edelhofer R, Prossinger H (2010) Infection with Toxoplasma gondii during pregnancy: seroepidemiological studies in Austria. Zoonoses Public Health 57:18–26
Ertug S, Okyay P, Turkmen M, Yuksel H (2005) Seroprevalence and risk factors for Toxoplasma infection among pregnant women in Aydin province, Turkey. BMC Public Health 5:66
Ezatpour B, Haghpanah B, Hejazi SH, Ghayour Z, Andalib AR (2009) Study of resistance to Toxoplasmosis using Tachizoites surface antigens of Toxoplasma gondii in vivo. Yafteh 10:63–70
Ezatpour B, Zibaie M, Rahmati H et al (2013) Seroprevalence of toxoplasmosis in mentally retarded patients in Iranian rehabilitation centers. J Parasit Dis 9(1):13–17. doi:10.1007/s12639-013-0358-6
Fallah E, Navazesh R, Majidi J, Kushavar H, Mahdipourzareh N (2005) An epidemiological study of Toxoplasma infection among high-school girls in Jolfa. J Reprod Infertil 6:261–269
Fallah M, Rabiee S, Matini M, Taherkhani H (2008) Seroepidemiology of toxoplasmosis in primigravida women in Hamadan, Islamic Republic of Iran, 2004. East Mediterr Health J 14:163–171
Goldstein EJ, Montoya JG, Remington JS (2008) Management of Toxoplasma gondii infection during pregnancy. Clin Infect Dis 47:554–566
Hajsoleimani F, Ataeian A, Nourian A, Mazloomzadeh S (2012) Seroprevalence of Toxoplasma gondii in pregnant women and bioassay of IgM positive cases in Zanjan, Northwest of Iran. Iran J Parasitol 7:82
Hashemi HJ, Saraei M (2010) Seroprevalence of Toxoplasma gondii in unmarried women in Qazvin, Islamic Republic of Iran. East Mediter Health J 16:24–28
Hung C-C, Fan C-K, Su K-E et al (2007) Serological screening and toxoplasmosis exposure factors among pregnant women in the Democratic Republic of Sao Tome and Principe. Trans R Soc Trop Med Hyg 101:134–139
Keshavarz H, Mamishi S, Daneshvar H (2000) The prevalence of Toxoplasma infection in hospitalized patients in selected hospitals of Kerman. J Kerman Univ Med Sci 7:129–136
Khatun S, Islam MN, Huq T (1998) Seroprevalence of Toxoplasma antibodies among the antenatal population in Bangladesh. J Obstet Gynaecol Res 24:115–119
Lappalainen M, Koskiniemi M, Hiilesmaa V et al (1995) Outcome of children after maternal primary Toxoplasma infection during pregnancy with emphasis on avidity of specific IgG. Pediat Infect Dis J 14:354–361
Mahmoudvand H, Saedi Dezaki E, Soleimani S, Baneshi MR, Kheirandish F, Ezatpour B, Zia-ali N (2015) Seroprevalence and risk factors of Toxoplasma gondii infection among healthy blood donors in southeast of Iran. Parasite Immunol 37:362–367
Mandell G, Dolin R, Bennett J, Mandell GL, Bennett J (2009) Mandell, Douglas, and Bennett’s principles and practice of infectious diseases. Elsevier, Amsterdam
Montoya JG (2002) Laboratory diagnosis of Toxoplasma gondii infection and toxoplasmosis. J Infect Dis 185:S73–S82
Mostafavi N, Ataei B, Nokhodian Z, Monfared LJ, Yaran M, Ataie M, Babak A (2012) Toxoplasma gondii infection in women of childbearing age of Isfahan, Iran: a population-based study. Adv Biomed Res 1:60
Nash J, Chissel S, Jones J, Warburton F, Verlander N (2005) Risk factors for toxoplasmosis in pregnant women in Kent, United Kingdom. Epidemiol Infect 133:475–483
Parvizpour F, Hajighasemlo S, Hasani S, Olfati L, Bahmani A, Hoseini F, Gharibi F (2010) Toxoplasmosis infection in the pregnant women in the first half of pregnancy, in Kamyaran in 2008. Sci J Kurd Univ Med Sci 15:72–78
Romand S, Wallon M, Franck J, Thulliez P, Peyron F, Dumon H (2001) Prenatal diagnosis using polymerase chain reaction on amniotic fluid for congenital toxoplasmosis. Obstet Gynecol 97:296–300
Sarkar MD, Anuradha B, Sharma N, Roy RN (2012) Seropositivity of toxoplasmosis in antenatal women with bad obstetric history in a tertiary-care hospital of Andhra Pradesh, India. J Health Popul Nutr 30:87
Shaapan R (2015) The common zoonotic protozoal diseases causing abortion. J Parasit Dis. doi:10.1007/s12639-015-0661-5
Shin D-W, Cha D-Y, Hua QJ, Cha G-H, Lee Y-H (2009) Seroprevalence of Toxoplasma gondii infection and characteristics of seropositive patients in general hospitals in Daejeon, Korea. Korean J Parasitol 47:125–130
Tekkesin N (2012) Diagnosis of toxoplasmosis in pregnancy: a review. HOAJ Biol 1:9
Varella IS, Wagner MB, Darela AC, Nunes LM, Müller RW (2003) Seroprevalence of toxoplasmosis in pregnant women. J Pediatria 79:69–74
Yad MJY, Jomehzadeh N, Sameri MJ, Noorshahi N (2014) Seroprevalence of Anti-Toxoplasma gondii antibodies among pregnant woman in South Khuzestan, Iran. Jundishapur J Microbiol 7:e9998
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The present work was based on a thesis defended at the Tehran University of Medical Sciences (TUMS). This study was supported by TUMS Grant.
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Ahmadpour, G.R., Ezatpour, B., Hadighi, R. et al. Seroepidemiology of Toxoplasma gondii infection in pregnant women in west Iran: determined by ELISA and PCR analysis. J Parasit Dis 41, 237–242 (2017). https://doi.org/10.1007/s12639-016-0784-3
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DOI: https://doi.org/10.1007/s12639-016-0784-3