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The Inhibition Effect of Lactobacilli Against Growth and Biofilm Formation of Pseudomonas aeruginosa

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Abstract

The emergence of antibiotic-resistant and food-spoilage microorganisms has renewed efforts to identify safe and natural alternative agents of antibiotics such as probiotics. The aim of this study was the isolation of lactobacilli as potential probiotics from local dairy products with broad antibacterial and anti-biofilm activities against antibiotic-resistant strains of Pseudomonas aeruginosa and determination of their inhibition mechanism. Antibiotic susceptibility and classification of acquired resistance profiles of 80 P. aeruginosa strains were determined based on Centers for Disease Control and Prevention (CDC) new definition as multidrug-resistant (MDR), extensively drug-resistant (XDR), and pan-drug-resistant (PDR) followed by antibacterial assessment of lactobacilli against them by different methods. Among the 80 P. aeruginosa strains, 1 (1.3%), 50 (62.5%), and 78 (97.5%) were PDR, XDR, and MDR, respectively, and effective antibiotics against them were fosfomycin and polymyxins. Among 57 isolated lactobacillus strains, two strains which were identified as Lactobacillus fermentum using biochemical and 16S rDNA methods showed broad inhibition/killing and anti-biofilm effects against all P. aeruginosa strains. They formed strong biofilms and had bile salts and low pH tolerance. Although investigation of inhibition mechanism of these strains showed no bacteriocin production, results obtained by high-performance liquid chromatography (HPLC) analysis indicated that their inhibitory effect was the result of production of three main organic acids including lactic acid, acetic acid, and formic acid. Considering the broad activity of these two L. fermentum strains, they can potentially be used in bio-control of drug-resistant strains of P. aeruginosa.

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Acknowledgments

The authors are grateful to Afsaneh Shokri and Fatemeh Khodabakhsh for their valuable help in editing of paper.

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Authors and Affiliations

  1. Department of Biology, Faculty of Sciences, University of Isfahan, Hezar Jarib Street, Isfahan, Iran

    Dariush Shokri & Mohammad Rabbani Khorasgani

  2. Department of Pharmaceutical Biotechnology and Pharmaceutical Sciences Research Center, Faculty of Pharmacy, Shiraz University of Medical Sciences, Shiraz, Iran

    Milad Mohkam & Younes Ghasemi

  3. Faculty of Basic Sciences, Department of Microbiology, Islamic Azad University, Shahrekord Branch, Shahrekord, Iran

    Seyed Masih Fatemi

  4. Department of Biotechnology, Faculty of Advanced Sciences and Technologies, University of Isfahan, Isfahan, Iran

    Asghar Taheri-Kafrani

Authors
  1. Dariush Shokri
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  2. Mohammad Rabbani Khorasgani
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  3. Milad Mohkam
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  4. Seyed Masih Fatemi
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  5. Younes Ghasemi
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Correspondence to Mohammad Rabbani Khorasgani.

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Funding

This study was funded by the University of Isfahan and Iran National Science Foundation, Presidency of Islamic Republic of Iran Vice-Presidency for Science and Technology (grant number: 92045033).

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This article does not contain any studies with human participants or animals performed by any of the authors.

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Shokri, D., Khorasgani, M.R., Mohkam, M. et al. The Inhibition Effect of Lactobacilli Against Growth and Biofilm Formation of Pseudomonas aeruginosa . Probiotics & Antimicro. Prot. 10, 34–42 (2018). https://doi.org/10.1007/s12602-017-9267-9

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  • DOI: https://doi.org/10.1007/s12602-017-9267-9

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