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LPS-induced NO inhibition and antioxidant activities of ethanol extracts and their solvent partitioned fractions from four brown seaweeds

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Abstract

The nitric oxide inhibitory (NOI) and antioxidant (ABTS and DPPH radical scavenging effects with reducing power) activities of the ethanol (EtOH) extracts and solvent partitioned fractions from Scytosiphon lomentaria, Chorda filum, Agarum cribrosum, and Desmarestia viridis were investigated, and the correlation between biological activity and total phenolic (TP) and phlorotannin (TPT) content was determined by PCA analysis. The yield of EtOH extracts from four brown seaweeds ranged from 2.6 to 6.6% with the highest yield from D. viridis, and the predominant compounds in their solvent partitioned fractions had medium and/or less polarity. The TP and TPT content of the EtOH extracts were in the ranges of 25.0–44.1 mg GAE/g sample and 0.2–4.6 mg PG/g sample, respectively, which were mostly included in the organic solvent partitioned fractions. Strong NOI activity was observed in the EtOH extracts and their solvent partitioned fractions from D. viridis and C. filum. In addition, the EtOH extract and its solvent partitioned fractions of D. viridis exhibited little cytotoxicity to Raw 264.7 cells. The most potent ABTS and DPPH radical scavenging capacity was shown in the EtOH extracts and their solvent partitioned fractions from S. lomentaria and C. filum, and both also exhibited strong reducing ability. In the PCA analysis the content of TPT had a good correlation with DPPH (r = 0.62), ABTS (r = 0.69) and reducing power (r = 0.65), however, an unfair correlation was observed between the contents of TP and TPT and NOI, suggesting that the phlorotannins might be responsible for the DPPH and ABTS radical scavenging activities.

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Authors and Affiliations

  1. East Sea Research Institute, KIOST, Uljin, 767-813, Korea

    Myoung Lae Cho

  2. Department of Marine Food Science and Technology, College of Life Sciences, Gangneung-Wonju National University, Gangneung, 210-702, Korea

    Dong-Jin Lee & Sang Guan You

  3. Global Bioresources Research Center, KIOST, Ansan, 426-744, Korea

    Hyi-Seung Lee & Yeon-Ju Lee

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  1. Myoung Lae Cho
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  2. Dong-Jin Lee
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  3. Hyi-Seung Lee
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  4. Yeon-Ju Lee
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  5. Sang Guan You
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Correspondence to Sang Guan You.

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Cho, M.L., Lee, DJ., Lee, HS. et al. LPS-induced NO inhibition and antioxidant activities of ethanol extracts and their solvent partitioned fractions from four brown seaweeds. Ocean Sci. J. 48, 349–359 (2013). https://doi.org/10.1007/s12601-013-0033-y

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  • DOI: https://doi.org/10.1007/s12601-013-0033-y

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