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Effect of low temperature on the distribution of Chrysanthemum stunt viroid in Chrysanthemum morifolium

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Abstract

Replication and cellular movement of viroids are dependent on host plant components. Low temperatures reduce the physiological activity of host plants and thus suppress the levels of viroids. Chrysanthemum stunt viroid (CSVd) concentration was reduced in infected chrysanthemum (Chrysanthemum morifolium) plants subjected to low-temperature treatment compared with untreated plants. In this study, we investigated the distribution patterns of CSVd in the developing shoot tips of CSVd-infected chrysanthemum plants subjected to one-month, three-month, and six-month low-temperature treatment using in situ hybridization. After one month of low-temperature treatment, strong CSVd signals were detected in leaf primordia (LP) and the apical dome (AD); after three months, weak CSVd signals were detected in LP and the AD; while after six months, CSVd signals were not detected in LP or the AD, but only in tissues within the vascular bundle directly beneath viroid-free LP and AD areas. These changes in the distribution of CSVd in shoot tips subjected to low-temperature treatment might result from a reduction in viroid replication and/or the inhibition of viroid intra- and intercellular movement.

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Authors and Affiliations

  1. NARO Institute of Floricultural Science, Tsukuba, 305-8519, Japan

    Yosuke Matsushita

  2. Toyama Prefectural Agricultural, Forestry, and Fisheries Research Center, Toyama, 939-1327, Japan

    Yoshiteru Shima

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  1. Yosuke Matsushita
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  2. Yoshiteru Shima
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Correspondence to Yosuke Matsushita.

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Matsushita, Y., Shima, Y. Effect of low temperature on the distribution of Chrysanthemum stunt viroid in Chrysanthemum morifolium . Phytoparasitica 43, 609–614 (2015). https://doi.org/10.1007/s12600-015-0490-1

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  • DOI: https://doi.org/10.1007/s12600-015-0490-1

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