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Anti-inflammatory effects of sinapic acid on 2,4,6-trinitrobenzenesulfonic acid-induced colitis in mice

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Abstract

This study was conducted to evaluate the effect of sinapic acid, a cinnamic acid derivative, on inflammatory changes in a mouse model of colitis. Colitis was induced by intracolonic instillation of 2,4,6-trinitrobenzenesulfonic acid (TNBS). Sinapic acid (10, 30, and 100 mg/kg) and dexamethasone (2 mg/kg) were orally administered to Balb/c female mice after TNBS instillation. The anti-inflammatory effect of sinapic acid on colonic injury or damage was assessed by clinical, macroscopic, microscopic, and biochemical analyses. Compared with TNBS control, treatment with sinapic acid significantly improved colonic weight and length and decreased the macroscopic and microscopic changes in TNBS-induced colitis. Furthermore, myeloperoxidase activity and the colonic tissue levels of malondialdehyde and tumor necrosis factor alpha were decreased by administration of sinapic acid. The findings of this study suggest that sinapic acid exerts anti-inflammatory effects on intestinal inflammation and can be selected as a novel therapeutic candidate in the treatment of inflammatory bowel disease.

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References

  • Chachu KA, Osterman MT (2016) How to diagnose and treat IBD mimics in the refractory IBD patient who does not have IBD. Inflamm Bowel Dis 22:1262–1274

    Article  PubMed  Google Scholar 

  • Chen C (2016) Sinapic acid and its derivates as medicine in oxidative stress-induced diseases and aging. Oxid Med Cell Longev. https://doi.org/10.1155/2016/3571614

    Google Scholar 

  • Cuvelier ME, Richard H, Berset C (1992) Comparison of the antioxidative activity of some acid-phenols—structure–activity relationship. Biosci Biotechnol Biochem 56:324–325

    Article  CAS  Google Scholar 

  • Duerr RH, Taylor KD, Brant SR, Rioux JD, Silverberg MS, Daly MJ, Steinhart AH, Abraham C, Regueiro M, Griffiths A, Dassopoulos T, Bitton A, Yang H, Targan S, Datta LW, Kistner EO, Schumm LP, Lee AT, Gregersen PK, Barmada MM, Rotter JI, Nicolae DL, Cho JH (2006) A genome-wide association study identifies IL23R as an inflammatory bowel disease gene. Science 314:1461–1463

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Duvnjak M, Bilic A, Barsic N, Tomasic V, Stojsavljevic S (2013) Classical medications in the treatment of inflammatory bowel diseases. Acta Med Croatica 67:11–124

    Google Scholar 

  • Fukuda W, Takagi T, Katada K, Mizushima K, Okayama T, Yoshida N, Kamada K, Uchiyama K, Ishikawa T, Handa O, Konishi H, Yagi N, Ichikawa H, Yoshikawa T, Cepinskas G, Naito Y, Itoh Y (2014) Anti-inflammatory effects of carbon monoxide-releasing molecule on trinitrobenzene sulfonic acid-induced colitis in mice. Dig Dis Sci 59:1142–1151

    Article  CAS  PubMed  Google Scholar 

  • Galano A, Francisco-Marquez M, Alvarez-Idaboy JR (2011) Mechanism and kinetics studies on the antioxidant activity of sinapinic acid. Phys Chem Chem Phys 13:11199–11205

    Article  CAS  PubMed  Google Scholar 

  • Gan XL, Hei ZQ, Huang HQ, Chen LX, Li SR, Cai J (2006) Effect of Astragalus membranaceus injection on the activity of the intestinal mucosal mast cells after hemorrhagic shock-reperfusion in rats. Chin Med J (Engl) 119:1892–1898

    Google Scholar 

  • Hooper KM, Barlow PG, Stevens C, Henderson P (2017) Inflammatory bowel disease drugs: a focus on autophagy. J Crohns Colitis 11:118–127

    Article  PubMed  Google Scholar 

  • Jia Y, Guan Q, Jiang Y, Salh B, Guo Y, Tu P, Du C (2014) Amelioration of dextran sulphate sodium-induced colitis in mice by echinacoside-enriched extract of Cistanche tubulosa. Phytother Res 28:110–119

    Article  CAS  PubMed  Google Scholar 

  • Katz LH, Kopylov U, Fudim E, Yavzori M, Picard O, Ungar B, Eliakim R, Ben-Horin S, Chowers Y (2014) Expression of IL-2, IL-17 and TNF-alpha in patients with Crohn’s disease treated with anti-TNF antibodies. Clin Res Hepatol Gastroenterol 38:491–498

    Article  CAS  PubMed  Google Scholar 

  • Kim DH, Yoon BH, Jung WY, Kim JM, Park SJ, Park DH, Huh Y, Park C, Cheong JH, Lee KT, Shin CY, Ryu JH (2010) Sinapic acid attenuates kainic acid-induced hippocampal neuronal damage in mice. Neuropharmacology 59:20–30

    Article  CAS  PubMed  Google Scholar 

  • Liu X, Wang J (2011) Anti-inflammatory effects of iridoid glycosides fraction of Folium syringae leaves on TNBS-induced colitis in rats. J Ethnopharmacol 133:780–787

    Article  CAS  PubMed  Google Scholar 

  • Luceri C, Guglielmi F, Lodovici M, Giannini L, Messerini L, Dolara P (2004) Plant phenolic 4-coumaric acid protects against intestinal inflammation in rats. Scand J Gastroenterol 39:1128–1133

    CAS  PubMed  Google Scholar 

  • Menchen LA, Colon AL, Moro MA, Leza JC, Lizasoain I, Menchen P, Alvarez E, Lorenzo P (2001) N-(3-(aminomethyl)benzyl)acetamidine, an inducible nitric oxide synthase inhibitor, decreases colonic inflammation induced by trinitrobenzene sulphonic acid in rats. Life Sci 69:479–491

    Article  CAS  PubMed  Google Scholar 

  • Morris GP, Beck PL, Herridge MS, Depew WT, Szewczuk MR, Wallace JL (1989) Hapten-induced model of chronic inflammation and ulceration in the rat colon. Gastroenterology 96:795–803

    Article  CAS  PubMed  Google Scholar 

  • Niwa T, Doi U, Kato Y, Osawa T (1999) Inhibitory mechanism of sinapinic acid against peroxynitrite-mediated tyrosine nitration of protein in vitro. FEBS Lett 459:43–46

    Article  CAS  PubMed  Google Scholar 

  • Ohkawa H, Ohishi N, Yagi K (1979) Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Anal Biochem 95:351–358

    Article  CAS  PubMed  Google Scholar 

  • Park SY, Neupane GP, Lee SO, Lee JS, Kim MY, Kim SY, Park YJ, Kim JA (2014) Protective effects of Pogostemon cabin Bentham water extraction on inflammatory cytokine expression in TNBS-induced colitis in rats. Arch Pharm Res 37:253–262

    Article  CAS  PubMed  Google Scholar 

  • Podolsky DK (2002) Inflammatory bowel disease. N Engl J Med 347:417–429

    Article  CAS  PubMed  Google Scholar 

  • Sadar SS, Vyawahare NS, Bodhankar SL (2016) Ferulic acid ameliorates TNBS-induced ulcerative colitis through modulation of cytokines, oxidative stress, iNOS, COX-2, and apoptosis in laboratory rats. EXCLI J 15:482–499

    PubMed  PubMed Central  Google Scholar 

  • Sartor RB, Cromartie WJ, Powell DW, Schwab JH (1985) Granulomatous enterocolitis induced in rats by purified bacterial cell wall fragments. Gastroenterology 89:587–595

    Article  CAS  PubMed  Google Scholar 

  • Van Meeteren ME, Meijssen MA, Zijlstra FJ (2000) The effect of dexamethasone treatment on murine colitis. Scand J Gastroenterol 35:517–521

    Article  PubMed  Google Scholar 

  • Vetuschi A, Latella G, Sferra R, Caprilli R, Gaudio E (2002) Increased proliferation and apoptosis of colonic epithelial cells in dextran sulfate sodium-induced colitis in rats. Dig Dis Sci 47:1447–1457

    Article  PubMed  Google Scholar 

  • Wallace JL (1988) Release of platelet-activating factor (PAF) and accelerated healing induced by a PAF antagonist in an animal model of chronic colitis. Can J Physiol Pharmacol 66:422–425

    Article  CAS  PubMed  Google Scholar 

  • Wang YH, Dong J, Zhang JX, Zhai J, Ge B (2016) Effects of mimic of manganese superoxide dismutase on 2,4,6-trinitrobenzene sulfonic acid-induced colitis in rats. Arch Pharm Res 39:1296–1306

    Article  CAS  PubMed  Google Scholar 

  • Zaotrski H, Sałaga M, Zielińska M, Piechota-Polańczyk A, Owczarek K, Kordek R, Lewandowska U, Chen C, Fichna J (2015) Experimental colitis in mice is attenuated by topical administration of chlorogenic acid. Naunyn Schmiedebergs Arch Pharmacol 388:643–651

    Article  Google Scholar 

  • Zhang Z, Wu X, Cao S, Wang L, Wang D, Yang H, Feng Y, Wang S, Li L (2016) Caffeic acid ameliorates colitis in association with increased Akkermansia population in the gut microbiota of mice. Oncotarget 7:31790–31799

    PubMed  PubMed Central  Google Scholar 

  • Zou Y, Dai SX, Chi HG, Li T, He ZW, Wang J, Ye CG, Huang GL, Zhao B, Li WY, Wan Z, Feng JS, Zheng XB (2015) Baicalin attenuates TNBS-induced colitis in rats by modulating the Th1/Treg paradigm. Arch Pharm Res 38:1873–1887

    Article  CAS  PubMed  Google Scholar 

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Acknowledgements

This research was supported by the Chung-Ang University Research Grants in 2015.

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  1. College of Pharmacy, Chung-Ang University, 84 Heukseok-ro, Dongjak-gu, Seoul, 06974, Republic of Korea

    Ji-Yun Lee

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Lee, JY. Anti-inflammatory effects of sinapic acid on 2,4,6-trinitrobenzenesulfonic acid-induced colitis in mice. Arch. Pharm. Res. 41, 243–250 (2018). https://doi.org/10.1007/s12272-018-1006-6

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  • DOI: https://doi.org/10.1007/s12272-018-1006-6

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