Abstract
The paired box domain gene 5 (PAX5) is frequently altered in both childhood and adult B-cell acute lymphoblastic leukemia, and takes part in leukemogenesis. We analyzed data from the database of Gene Expression Omnibus (accession number: GSE11877) using bioinformatical and statistical methods. The results showed that cases of PAX5 alteration can cluster using unsupervised clustering algorithms, and one gene, zinc and ring finger 1 (ZNRF1), was characterized and validated by quantitative RT-PCR. ZNRF1 may be associated with leukemogenesis of ALL with PAX5 alteration.
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12185_2013_1309_MOESM1_ESM.tif
Supplementary material 1 PAX5 deletions in B-ALL patients. Deletions were analyzed on 2% agarose gel. M is a 100 bp DNA ladder, P1 have strong Δ7/8/9 expression besides full length (FL). P2 have Δ7/8 but have no FL expression.P3, P4 and P6 have FL and Δ7/8 and P5 have FL, Δ8, Δ7/8. (TIFF 169 kb)
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Shang, Z., Zhao, Y., Zhou, K. et al. PAX5 alteration-associated gene-expression signatures in B-cell acute lymphoblastic leukemia. Int J Hematol 97, 599–603 (2013). https://doi.org/10.1007/s12185-013-1309-9
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DOI: https://doi.org/10.1007/s12185-013-1309-9