Abstract
ANXA2(AnnexinA2), a calcium-dependent phospholipid binding protein, is involved in various Ca2+-related biological activities. In the present study, full-length cDNA of ANXA2 was isolated from the velvet antler tip tissue of sika deer (Cervus nippon hortulorum); the amino acid sequence and gene expression was analyzed by using bioinformatics and real-time reverse transcriptase polymerase chain reaction (RT-PCR) techniques. Nucleotide sequence analysis reveals that the full-length cDNA of the ANXA2 gene was 1372 bp, of which 1020 bp was in the open-reading frame (ORF) encoding 339 amino acids; its relative molecular weight was 38.3 kDa; and isoelectric point was 6.72. Sequence analysis indicates that the protein includes four conserved tandem-duplication ANX domains. The gene-accession nucleotide sequence number in GenBank is JX315571. Expression analysis by RT-PCR reveals that ANXA2 gene expression has a significant positive correlation with the antler-tissue mineralization process, indicating that this gene may play an important role in the regulation of antler-tissue mineralization.
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Project funding: This research was funded by the General Program of the National Natural Science Foundation of China (31271324), and the Fundamental Research Funds for the Central Universities (2572014EA05-03), and the Fundamental Research Funds for the Central Universities (DL10BA08).
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Hao, L., Xiao, X. & Li, H. Full length cDNA cloning and expression analysis of annexinA2 gene from deer antler tissue. Journal of Forestry Research 25, 953–957 (2014). https://doi.org/10.1007/s11676-014-0542-2
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DOI: https://doi.org/10.1007/s11676-014-0542-2