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MiR-126-3p promotes the cell proliferation and inhibits the cell apoptosis by targeting TSC1 in the porcine granulosa cells

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Abstract

In mammalian ovaries, many studies demonstrated that the proliferation and apoptosis of granulosa cells are involved in folliculogenesis. Previous evidence suggests that miR-126-3p might get involved in the proliferation and apoptosis of granulosa cells, and tuberous sclerosis complex 1 (TSC1) gene was predicted as one target of miR-126-3p, and moreover, granulosa cell-specific TSC1 knockout stimulated folliculogenesis in mice. However, the molecular regulation of miR-126-3p on TSC1 and its effects on cell proliferation and apoptosis remain virtually unexplored in granulosa cells. Using porcine granulosa cells as a model, the luciferase report assay, mutation, deletion, Annexin-V/PI staining, and EdU assays were applied to investigate the molecular mechanism for miR-126-3p regulating the expression of TSC1 and their effects on the cell proliferation and apoptosis. We found that miR-126-3p showed a positive effect on cell proliferation and a negative effect on cell apoptosis in porcine granulosa cells, and knockdown of TSC1 significantly promoted cell proliferation and significantly inhibited cell apoptosis in porcine granulosa cells. Furthermore, miR-126-3p might target and repress the expressions of TSC1 at the post-transcriptional level, thereby promoting cell proliferation and inhibiting cell apoptosis of granulosa cells. These findings would provide of great insight in further exploring the molecular regulation of miR-126-3p and TSC1 on the functions of granulosa cells during the folliculogenesis in mammals.

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Funding

This work was supported by the earmarked fund for the China Agriculture Research System (CARS-35), the Basic Work of Science and Technology Project (2014FY120800), and Guangdong Sailing Program (2014YT02H042).

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Author notes

  1. Xiaolong Yuan and Xi Deng contributed equally to this work.

Authors and Affiliations

  1. Guangdong Provincial Key Lab of Agro-Animal Genomics and Molecular Breeding, National Engineering Research Centre for Breeding Swine Industry, College of Animal Science, South China Agricultural University, Guangzhou, Guangdong, China

    Xiaolong Yuan, Xi Deng, Xiaofeng Zhou, Yan Xing, Zhe Zhang, Hao Zhang & Jiaqi Li

  2. College of Biological and Food Engineering, Guangdong University of Education, Guangzhou, Guangdong, China

    Ailing Zhang

Authors
  1. Xiaolong Yuan
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  2. Xi Deng
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  3. Xiaofeng Zhou
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  4. Ailing Zhang
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  5. Yan Xing
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  6. Zhe Zhang
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  7. Hao Zhang
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  8. Jiaqi Li
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Contributions

Conceptualization: Xiaolong Yuan and Jiaqi Li

Investigation: Xi Deng, Xiaofeng Zhou, Ailing Zhang, and Yan Xin

Writing – original draft: Xiaolong Yuan

Writing – review and editing: Zhe Zhang, Hao Zhang, and Jiaqi Li

All authors read and approved the final manuscript.

Corresponding author

Correspondence to Jiaqi Li.

Ethics declarations

All experiments in the present study were performed in accordance with the guidelines of the Animal Care and Use Committee of South China Agricultural University Guangzhou, China (approval number: SCAU#2013-10).

Competing interest

The authors declare that they have no competing interests.

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Editor: Tetsuji Okamoto

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Yuan, X., Deng, X., Zhou, X. et al. MiR-126-3p promotes the cell proliferation and inhibits the cell apoptosis by targeting TSC1 in the porcine granulosa cells. In Vitro Cell.Dev.Biol.-Animal 54, 715–724 (2018). https://doi.org/10.1007/s11626-018-0292-0

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  • DOI: https://doi.org/10.1007/s11626-018-0292-0

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