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Diversity of Colletotrichum spp. isolated from chili pepper fruit exhibiting symptoms of anthracnose in Thailand

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Abstract

Colletotrichum spp. are causal agents of anthracnose disease in chili fruits and other tropical crops. The disease is increasing in chili fruits in Thailand and significantly reduces fruit quality and fruit production. Forty-eight isolates of Colletotrichum spp. associated with chili anthracnose were collected from different areas of Thailand during 2010–2015. Based on morphological characteristic identification, 10 isolates were shown to belong to the C. gloeosporioides species complex, 24 isolates belong to the C. acutatum species complex and 14 isolates to C. capsici. For molecular identification, two primer sets, ITS1/ITS4 and ACT528/ACT738, were used for amplification of the internal transcribed spacer of rRNA gene (ITS1–5.8S–ITS2) and partial region actin gene (ACT), respectively. The phylogenetic analysis of individual and combined ITS region and actin nucleotide sequences identified the collected isolates into 4 species: C. gloeosporioides, C. siamense, C. acutatum and C. capsici. The pathogenicity test demonstrated that all four species were pathogenic on intact unwounded and healthy fruits. These results indicated that C. capsici, C. acutatum, C. gloeosporioides and C. siamense were the causal agents of chili anthracnose disease.

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Acknowledgements

In this work, we wish to acknowledge the financial support from the Thailand Research Fund (TRF) through the Royal Golden Jubilee (RGJ) Ph.D. program (Grant No. PHD/0158/2551). The authors acknowledge the Department of Plant Pathology, Kasetsart University for laboratory facilities and the cooperation of the many Thailand chili growers who participated in this study.

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Authors and Affiliations

  1. Department of Plant Pathology, Faculty of Agriculture, Kasetsart University, 50 Ngamwongwan Road, Chatuchak, Bangkok, 10900, Thailand

    Sawita Suwannarat, Pattavipha Songkumarn & Somsiri Sangchote

  2. Division of Plant Protection, Department of Crop Science, University of Natural Resources and Life Sciences Vienna, Konrad-Lorenz-Strasse 24, 3430, Tulln, Austria

    Siegrid Steinkellner

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  1. Sawita Suwannarat
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  2. Siegrid Steinkellner
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  3. Pattavipha Songkumarn
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  4. Somsiri Sangchote
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Correspondence to Somsiri Sangchote.

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Section editor: Gerhard Rambold

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Suwannarat, S., Steinkellner, S., Songkumarn, P. et al. Diversity of Colletotrichum spp. isolated from chili pepper fruit exhibiting symptoms of anthracnose in Thailand. Mycol Progress 16, 677–686 (2017). https://doi.org/10.1007/s11557-017-1304-2

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  • DOI: https://doi.org/10.1007/s11557-017-1304-2

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