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In vitro differentiation of mouse ES cells into hepatocytes with coagulation factors VIII and IX expression profiles

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Abstract

Coagulation factors II, V, VII, VIII, IX and X are produced by hepatocytes. So factors VIII and IX deficiencies, which result in hemophilia A and B, have the potential to respond to cellular replacement therapy. Embryonic stem (ES) cells provide a unique source for therapeutic applications. Here, E14 mouse ES cells have been induced into hepatocytes in vitro. Morphology revealed that ES-derived hepatic-like cells were round or polyhedral shaped with distinct boundary of individual cells, and some arranged in trabeculae. These cells expressed endodermal-or liver-specific mRNA—transthyretin (TTR), α1-anti-trypsin (AAT), α-fetoprotein (AFP), albumin (ALB), glucose-6-phoshpatase (G6P) and tyrosine aminotransferase (TAT). Approximately (85.1±0.5)% of the ES-derived cells was stained positive green with ICG uptake. These cells were also stained magenta as a result of PAS reaction. In this paper, expression of coagulation factors VIII and IX mRNA in the ES-derived cells is documented. Therefore, ES cells might be developed as substitute donor cells for the therapy of coagulation factor deficiencies.

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Authors and Affiliations

  1. Center for Stem Cell Research, the Second Affiliated Hospital of Sun Yat-sen University, Guangzhou, 510120, China

    Meng Ying, Huang Shaoliang & Min Jun

  2. Department of Pediatrics, the Second Affiliated Hospital of Sun Yat-sen University, Guangzhou, 510120, China

    Huang Shaoliang

  3. Department of Surgery, the Second Affiliated Hospital of Sun Yat-sen University, Guangzhou, 510120, China

    Min Jun

  4. Experimental Animal Center, Sun Yat-sen Medical College, Sun Yat-sen University, Guangzhou, 510089, China

    Guo Zhongmin

Authors
  1. Meng Ying
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  2. Huang Shaoliang
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  3. Min Jun
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  4. Guo Zhongmin
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Correspondence to Meng Ying.

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Meng, Y., Huang, S., Min, J. et al. In vitro differentiation of mouse ES cells into hepatocytes with coagulation factors VIII and IX expression profiles. SCI CHINA SER C 49, 259–264 (2006). https://doi.org/10.1007/s11427-006-0259-3

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  • DOI: https://doi.org/10.1007/s11427-006-0259-3

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