Skip to main content
SpringerLink
Log in

Biotreatment of restaurant wastewater with an oily high concentration by newly isolated bacteria from oily sludge

  • Original Paper
  • Published:
World Journal of Microbiology and Biotechnology Aims and scope Submit manuscript

Abstract

High concentration restaurant oily wastewater from restaurants and food processing industries discharged into water environment usually results in environment pollution and inhibits the activity of microorganisms in biological wastewater treatment systems. In this study, 75 strains from oily sludge were isolated with oil degradation activity for edible oil-contained wastewater. Eight isolates were able to grow well in liquid cultures with edible oil as the sole carbon source and discovered with high efficient oil-degrading ability. Seven out of eight isolates were identified as Acinetobacter and one isolate as Kluyvera cryocrescens, based on their 16S rRNA gene sequences. Three highly efficient oil degrading bacteria (Acinetobacter dijkshoorniae LYC46-2, Kluyvera cryocrescens LYC50-1a and Acinetobacter pittii LYC73-4b) were selected and their degradation characteristic were examined, the results showed that the three isolates were effective under pH range from 7.0 to 10.0, and temperature from 25 to 35 °C. For degradation of 2–4% (v/v) of vegetable oil, > 85% degradation percentage were obtained within 30 h. Degradation of the higher concentration oil (6–8%, v/v) result in 50–70% degradation percentage within 72 h, and the degradation percentage for the isolated strains were decreased about 50% for the degradation of 10% oil (< 45%) compared to 2% oil. Different type of oils were also tested, > 90% of degradation percentage were obtained by the three isolates, implied that these strains are capable of removing various oils efficiently. These results suggested that Acinetobacter dijkshoorniae LYC46-2, Kluyvera cryocrescens LYC50-1a and Acinetobacter pittii LYC73-4b are potential species could be efficiently used for high concentration restaurant oily wastewater treatment and might be applicable to a wastewater treatment system for the removal of oil.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5

Similar content being viewed by others

References

  • Abalos A, Viñas M, Sabaté J, Manresa MA, Solanas AM (2004) Enhanced biodegradation of Casablanca crude oil by a microbial consortium in presence of a rhamnolipid produced by Pseudomonas aeruginosa AT10. Biodegradation 15:249–260

    Article  CAS  Google Scholar 

  • Adesodun JK, Mbagwu JS (2008) Biodegradation of waste-lubricating petroleum oil in a tropical alfisol as mediated by animal droppings. Bioresour Technol 99:5659–5665

    Article  CAS  Google Scholar 

  • Akiyama S (1991) Present situation and prospect of recovered oil use. Degradation of fat and oil by "Bacillus subtilis BN 1001". Success in keeping high concentration of BN clean. Yushi 44:46–51

    Google Scholar 

  • Aluyor EO, Obahiagbon KO, Orijesu M (2009) Biodegradation of vegetable oils: a review. Sci Res Essays 4:543–548

    Google Scholar 

  • Atagana H, Haynes R, Wallis FM (2003) The Use of surfactants as possible enhancers in bioremediation of creosote contaminated soil. Water Air Soil Pollut 142:137–149

    Article  CAS  Google Scholar 

  • Awasthi MK, Selvam A, Chan MT et al (2018) Bio-degradation of oily food waste employing thermophilic bacterial strains. Bioresour Technol 248:141–147

    Article  CAS  Google Scholar 

  • Brooksbank AM, Latchford JW, Mudge SM (2007) Degradation and modification of fats, oils and grease by commercial microbial supplements. World J Microbiol Biotechnol 23:977–985

    Article  CAS  Google Scholar 

  • Campo P, Zhao Y, Suidan MT, Venosa AD, Sorial GA (2007) Biodegradation kinetics and toxicity of vegetable oil triacylglycerols under aerobic conditions. Chemosphere 68:2054–2062

    Article  CAS  Google Scholar 

  • Canler JP, Royer C, Duchène P (2001) Aerobic biological treatment of grease from urban wastewater treatment plants. Water Sci Technol 44:219–226

    Article  CAS  Google Scholar 

  • Chanthamalee J, Wongchitphimon T, Luepromchai E (2013) Treatment of oily bilge water from small fishing vessels by PUF-immobilized Gordonia sp. JC11. Water Air Soil Pollut 224:1601

    Article  Google Scholar 

  • Chen F, Yao Q (2015) The development of rural domestic wastewater treatment in China. Adv Mater Res 1073–1076:829–832

    Google Scholar 

  • Elbestawy E, Elmasry MH, Eladl NE (2005) The potentiality of free gram-negative bacteria for removing oil and grease from contaminated industrial effluents. World J Microbiol Biotechnol 21:815–822

    Article  CAS  Google Scholar 

  • Facchin S, Alves PDD, Siqueira FDF, Barroca TM, Victória JMN, Kalapothakis E (2013) Biodiversity and secretion of enzymes with potential utility in wastewater treatment. Open J Ecol 03:34–37

    Article  Google Scholar 

  • Hasanuzzaman M, Umadhay-Briones KM, Zsiros SM, Morita N, Nodasaka Y, Yumoto I, Okuyama H (2004) Isolation, identification, and characterization of a novel, oil-degrading bacterium, Pseudomonas aeruginosa T1. Curr Microbiol 49:108–114

    Article  CAS  Google Scholar 

  • Hassanshahian M, Emtiazi G, Cappello S (2012) Isolation and characterization of crude-oil-degrading bacteria from the Persian Gulf and the Caspian Sea. Mar Pollut Bull 64:7–12

    Article  CAS  Google Scholar 

  • Jiang Y, Qi H, Zhang X (2015) Novel method for separation and screening of lubricant-degrading microorganisms and bacterial biodegradation. Chin J Chem Eng 24:353–359

    Article  Google Scholar 

  • Joo HS, Ndegwa PM, Shoda M et al (2008) Bioremediation of oil-contaminated soil using Candida catenulata and food waste. Environ Pollut 156:891–896

    Article  CAS  Google Scholar 

  • Markossian S, Becker P, Markl H, Antranikian G (2000) Isolation and characterization of lipid-degrading Bacillus thermoleovorans IHI-91 from an icelandic hot spring. Extremophiles 4:365–371

    Article  CAS  Google Scholar 

  • Matsumiya Y, Wakita D, Kimura A, Sanpa S, Kubo M (2007) Isolation and characterization of a lipid-degrading bacterium and its application to lipid-containing wastewater treatment. J Biosci Bioeng 103:325–330

    Article  CAS  Google Scholar 

  • Matsuoka H, Miura A, Hori K (2009) Symbiotic effects of a lipase-secreting bacterium, Burkholderia arboris SL1B1, and a glycerol-assimilating yeast, Candida cylindracea SL1B2, on triacylglycerol degradation. J Biosci Bioeng 107:401–408

    Article  CAS  Google Scholar 

  • Montagnolli RN, Lopes PRM, Bidoia ED (2009) Applied models to biodegradation kinetics of lubricant and vegetable oils in wastewater. Int Biodeterior Biodegrad 63:297–305

    Article  CAS  Google Scholar 

  • Okuda SI, Ito K, Ozawa H, Izaki K (1991) Treatment of lipid-containing wastewater using bacteria which assimilate lipids. J Ferment Bioeng 71:424–429

    Article  CAS  Google Scholar 

  • Prasad MP, Manjunath K (2011) Comparative study on biodegradation of lipid-rich wastewater using lipase producing bacterial species. Indian J Biotechnol 10:121–124

    CAS  Google Scholar 

  • Pyrchenkova IA, Gafarov AB, Puntus IF, Filonov AE, Boronin AM (2006) Selection and characterization of active psychrotrophic microbial oil-degrading microorganisms. Appl Biochem Microbiol 42:263–269

    Article  CAS  Google Scholar 

  • Rahman KSM, Thahira-Rahman J, Lakshmanaperumalsamy P, Banat IM (2002) Towards efficient crude oil degradation by a mixed bacterial consortium. Bioresour Technol 85:257–261

    Article  CAS  Google Scholar 

  • Silva-Bedoya LM, Sánchez-Pinzón MS, Cadavid-Restrepo GE, Moreno-Herrera CX (2016) Bacterial community analysis of an industrial wastewater treatment plant in Colombia with screening for lipid-degrading microorganisms. Microbial Res 192:313–325

    Article  CAS  Google Scholar 

  • Sugimori D, Utsue T (2012) A study of the efficiency of edible oils degraded in alkaline conditions by Pseudomonas aeruginosa SS-219 and Acinetobacter sp. SS-192 bacteria isolated from Japanese soil. World J Microbiol Biotechnol 28:841–848

    Article  CAS  Google Scholar 

  • Sugimori D, Nakamura M, Mihara Y (2002) Microbial degradation of lipid by Acinetobacter sp. strain SOD-1. Biosci Biotechnol Biochem 66:1579–1582

    Article  CAS  Google Scholar 

  • Tanaka D, Takashima M, Mizuta A, Tanaka S, Sakatoku A, Nishikawa A, Osawa T, Noguchi M, Aizawa SI, Nakamura S (2010) Acinetobacter sp. Ud-4 efficiently degrades both edible and mineral oils: isolation and characterization. Curr Microbiol 60:203–209

    Article  CAS  Google Scholar 

  • Tano-Debrah K, Ohta Y (1994) Enzyme-assisted aqueous extraction of fat from kernels of the shea tree, butyrospermum parkii. J Am Oil Chem Soc 71:979–983

    Article  CAS  Google Scholar 

  • Tano-Debrah K, Fukuyama S, Otonari N, Taniguchi F, Ogura M (1999) An inoculum for the aerobic treatment of wastewaters with high concentrations of fats and oils. Bioresour Technol 69:133–139

    Article  CAS  Google Scholar 

  • Wahi R, Chuah LA, Choong TSY, Ngaini Z, Nourouzi MM (2013) Oil removal from aqueous state by natural fibrous sorbent: an overview. Sep Purif Technol 113:51–63

    Article  CAS  Google Scholar 

  • Wakelin NG, Forster CF (1997) An investigation into microbial removal of fats, oils and greases. Bioresour Technol 59:37–43

    Article  CAS  Google Scholar 

  • Wei Y, Van Houten RT, Borger AR, Eikelboom DH, Fan Y (2003) Minimization of excess sludge production for biological wastewater treatment. Water Res 37:4453–4467

    Article  CAS  Google Scholar 

  • Zhang GL, Wu YT, Qian XP, Meng Q (2005) Biodegradation of crude oil by Pseudomonas aeruginosa in the presence of rhamnolipids. J Zhejiang Univ Sci B 68:725–730

    Article  Google Scholar 

  • Zhang Z, Gai L, Hou Z, Yang C, Ma C, Wang Z, Sun B, He X, Tang H, Xu P (2010) Characterization and biotechnological potential of petroleum-degrading bacteria isolated from oil-contaminated soils. Bioresour Technol 101:8452–8456

    Article  CAS  Google Scholar 

  • Zhang H, Wang Q, Mortimer SR (2012) Waste cooking oil as an energy resource: review of Chinese policies. Renew Sustain Energy Rev 16:5225–5231

    Article  Google Scholar 

  • Zhao J, Yang K, Li B (2008) Problems and strategies for the market-oreiented collection and disposal of catering wastes in China's urban areas. Resour Sci 30:37–42

    Google Scholar 

Download references

Acknowledgements

This study was financially supported by the Key Research and Development (R&D) Projects of Shanxi Province (201703D32111270 and 201703D321009-4), Talent Training Project of Shanxi Postgraduate Joint Training Base (2018JD17) and Shanxi Scholarship Council of China (No. 2015–042).

Author information

Authors and Affiliations

  1. College of Environmental Science and Engineering, Taiyuan University of Technology, Taiyuan, 030024, Shanxi, People’s Republic of China

    Li-Li Gao & Yong-Chang Lu

  2. College of Mining Engineering, Taiyuan University of Technology, Taiyuan, 030024, Shanxi, People’s Republic of China

    Ji-Long Zhang

  3. Department of Biological and Pharmaceutical Engineering, College of Biomedical Engineering, Taiyuan University of Technology, No. 79 West Yingze Street, Taiyuan, 030024, Shanxi, China

    Jing Li & Jian-Dong Zhang

Authors
  1. Li-Li Gao
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Yong-Chang Lu
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Ji-Long Zhang
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Jing Li
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Jian-Dong Zhang
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Jian-Dong Zhang.

Ethics declarations

Conflict of interest

The authors declare that there is no conflict of interest.

Research involving with human participants

This study does not describe any experimental work related to human.

Additional information

Publisher's Note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Gao, LL., Lu, YC., Zhang, JL. et al. Biotreatment of restaurant wastewater with an oily high concentration by newly isolated bacteria from oily sludge. World J Microbiol Biotechnol 35, 179 (2019). https://doi.org/10.1007/s11274-019-2760-4

Download citation

  • Received:

  • Accepted:

  • Published:

  • DOI: https://doi.org/10.1007/s11274-019-2760-4

Keywords

Search

Navigation