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Transgenic rice expressing rice stripe virus NS3 protein, a suppressor of RNA silencing, shows resistance to rice blast disease

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Abstract

The NS3 protein of rice stripe virus (RSV), encoded by the virion strand of RNA3, is a viral suppressor of RNA silencing (VSR). Rice expressing NS3 had a normal phenotype, was initially sensitive to RSV but recovered at the later stages of infection. RSV accumulated slightly more in transgenic than in wild-type plants at the early stage of infection, but accumulation was similar later. Transgenic rice expressing NS3 also showed enhanced resistance to the fungus Magnaporthe oryzae. Meanwhile, expressional levels of genes related to the salicylic acid (SA) and jasmonic acid (JA) pathways were not significantly altered, indicating that the defense to M. oryzae was independent of the SA and JA pathways. We propose that NS3 may have dual functions, facilitating viral infection as a VSR and inhibiting pathogenic development as an inducer of host defense.

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Acknowledgments

This work was financially supported by the State Basic Research Program of China (2012CB722504), Special Fund for Agro-scientific Research in the Public Interest (201003031), the International Science & Technology Cooperation Program of China (2012DFA30900), the National Natural Science Foundation of China (31272016), the Program for Zhejiang Leading Team of Science and Technology Innovation, and the Program for Leading Team of Agricultural Research and Innovation of Ministry of Agriculture, China. We thank Professor M.J. Adams, Rothamsted Research, Harpenden, UK for correcting the English of the manuscript.

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Authors and Affiliations

  1. College of Agriculture and Biotechnology, Zhejiang University, Hangzhou, 310058, China

    Gentu Wu

  2. State Key Laboratory Breeding Base for Zhejiang Sustainable Pest and Disease Control, Zhejiang Academy of Agricultural Sciences, Hangzhou, 310021, China

    Gentu Wu, Jiaoyu Wang, Yong Yang, Bo Dong, Yanli Wang, Guochang Sun, Chengqi Yan, Fei Yan & Jianping Chen

  3. Institute of Plant Protection and Microbiology, Zhejiang Academy of Agricultural Sciences, Hangzhou, 310021, China

    Jiaoyu Wang, Yanli Wang & Guochang Sun

  4. Key Laboratory of Biotechnology in Plant Protection of Ministry of China, Institute of Virology and Biotechnology, Zhejiang Academy of Agricultural Sciences, Hangzhou, 310021, China

    Yong Yang, Bo Dong, Chengqi Yan, Fei Yan & Jianping Chen

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  1. Gentu Wu
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  2. Jiaoyu Wang
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  3. Yong Yang
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  4. Bo Dong
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  5. Yanli Wang
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  8. Fei Yan
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Corresponding authors

Correspondence to Fei Yan or Jianping Chen.

Electronic supplementary material

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11262_2014_1051_MOESM1_ESM.tif

NS3 transgenic rice plants and NS3 expression in 5 lines. a Diagram of the vector used for transformation; the CaMV 35S drives the NS3 coding sequence. b Transgenic (NS3) and wild type (WT) plants. c Northern blot showing expression of NS3 mRNA in five lines of NS3 transgenic plants.. Supplementary material 1 (TIFF 2869 kb)

Materials and methods . Supplementary material 2 (DOC 63 kb)

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Wu, G., Wang, J., Yang, Y. et al. Transgenic rice expressing rice stripe virus NS3 protein, a suppressor of RNA silencing, shows resistance to rice blast disease. Virus Genes 48, 566–569 (2014). https://doi.org/10.1007/s11262-014-1051-2

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  • DOI: https://doi.org/10.1007/s11262-014-1051-2

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