Skip to main content

Advertisement

SpringerLink
Log in

Engineering cotton (Gossypium hirsutum L.) for resistance to cotton leaf curl disease using viral truncated AC1 DNA sequences

  • Published:
Virus Genes Aims and scope Submit manuscript

An Erratum to this article was published on 22 April 2011

Abstract

Several important biological processes are performed by distinct functional domains found on replication-associated protein (Rep) encoded by AC1 of geminiviruses. Two truncated forms of replicase (tAC1) gene, capable of expressing only the N-terminal 669 bp (5′AC1) and C-terminal 783 bp (3′AC1) nucleotides cloned under transcriptional control of the CaMV35S were introduced into cotton (Gossypium hirsutum L.) using LBA4404 strain of Agrobacterium tumefaciens to make use of an interference strategy for impairing cotton leaf curl virus (CLCuV) infection in transgenic cotton. Compared with nontransformed control, we observed that transgenic cotton plants overexpressing either N-terminal (5′AC1) or C-terminal (3′AC1) sequences confer resistance to CLCuV by inhibiting replication of viral genomic and β satellite DNA components. Molecular analysis by Northern blot hybridization revealed high transgene expression in early and late growth stages associated with inhibition of CLCuV replication. Of the eight T1 transgenic lines tested, six had delayed and minor symptoms as compared to nontransformed control lines which developed disease symptoms after 2–3 weeks of whitefly-mediated viral delivery. Virus biological assay and growth of T2 plants proved that transgenic cotton plants overexpressing 5′- and 3′AC1 displayed high resistance level up to 72, 81%, respectively, as compared to non-transformed control plants following inoculation with viruliferous whiteflies giving significantly high cotton seed yield. Progeny analysis of these plants by polymerase chain reaction (PCR), Southern blotting and virus biological assay showed stable transgene, integration, inheritance and cotton leaf curl disease (CLCuD) resistance in two of the eight transgenic lines having single or two transgene insertions. Transgenic cotton expressing partial AC1 gene of CLCuV can be used as virus resistance source in cotton breeding programs aiming to improve virus resistance in cotton crop.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6

Similar content being viewed by others

References

  1. S. Mansoor, R.W. Briddon, Y. Zafar, J. Stanley, Trends Plant Sci. 8, 128 (2003)

    Article  PubMed  CAS  Google Scholar 

  2. A.S. Moffat, Science 286, 835 (1999)

    Article  Google Scholar 

  3. M.I. Boulton, Ann. Appl. Biol. 142, 143 (2003)

    Article  Google Scholar 

  4. M. Rahman, D. Hussain, Y. Zafar, Crop Sci. 42, 2137 (2002)

    Article  CAS  Google Scholar 

  5. R.W. Briddon, S. Mansoor, I.D. Bedford, M.S. Pinner, K. Saunders, J. Stanley, Y. Zafar, K.A. Malik, P.G. Markham, Virology 285, 234 (2001)

    Article  PubMed  CAS  Google Scholar 

  6. R.N. Beachy, Curr. Opin. Biotechnol. 8, 215 (1997)

    Article  PubMed  CAS  Google Scholar 

  7. P.V. Shivaprasad, V.B. Thillaichidambaram, K. Valuthambi, Virus Genes 33, 365 (2006)

    Article  PubMed  CAS  Google Scholar 

  8. S. Asad, W.A.A. Haris, A. Bashir, Y. Zafar, K.A. Malik, C.P. Lichtenstein, Arch. Virol. 148, 2341 (2003)

    Article  PubMed  CAS  Google Scholar 

  9. U. Ganeson, S.S. Suri, S. Rajasubraminiam, M.V. Rajam, I. Dasgupta, Virus Genes 39, 113 (2009)

    Article  Google Scholar 

  10. Y. Hong, J. Stanley, Mol. Plant Microbe Interact. 9, 219 (1996)

    Article  CAS  Google Scholar 

  11. P. Chellappan, M.V. Masona, R. Vanitharani, N.J. Taylor, C.M. Fauquet, Plant Mol. Biol. 56, 601 (2004)

    Article  PubMed  CAS  Google Scholar 

  12. S.F. Hanson, D.P. Maxwell, Phytopathology 89, 480 (1999)

    Article  PubMed  CAS  Google Scholar 

  13. A. Sangare, D. Deng, C.M. Fauquet, R.N. Beachy, Mol. Biol. Rep. 5, 95 (1999)

    CAS  Google Scholar 

  14. A.G. Day, E.R. Bejarano, K.W. Buck, M. Burrell, C.P. Lichtenstein, Proc. Natl. Acad. Sci. USA 88, 6721 (1991)

    Article  PubMed  CAS  Google Scholar 

  15. E.R. Bejarano, C.P. Lichtenstein, Plant Mol. Biol. 24, 241 (1994)

    Article  PubMed  CAS  Google Scholar 

  16. M. Bendahmane, B. Gronenborn, Plant Mol. Biol. 33, 351 (1997)

    Article  PubMed  CAS  Google Scholar 

  17. F.J.L. Aragao, S.G. Ribeiro, L.M.G. Barros, A.C.M. Brasiliero, D.P. Maxwell, E.L. Rech, J.C. Faria, Mol. Breed. 4, 491 (1998)

    Article  CAS  Google Scholar 

  18. E. Noris, G.P. Accotto, R. Tavazza, A. Brunetti, S. Crespi, M. Tavazza, Virolology 224, 130 (1996)

    Article  CAS  Google Scholar 

  19. T.A. Wilkins, R. Mishra, N.L. Trolinder, J. Food Agric. Environ. 2, 179 (2004)

    Google Scholar 

  20. J. Sambrook, F.F. Fritsch, T. Maniatis, Moleculat Cloning: A Laboratory Manual, 2nd edn. (Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, 1989), pp. 1.3–1.4

    Google Scholar 

  21. S. Asad, Z. Mukhtar, F. Nazir, J.A. Hashmi, S. Mansoor, Y. Zafar, M. Arshad, Mol. Biotechnol. 40, 161 (2008)

    Article  PubMed  CAS  Google Scholar 

  22. J.D.G. Jones, Curr. Opin. Plant Biol. 4, 281 (2001)

    Article  PubMed  CAS  Google Scholar 

  23. R.M. Solomon-Blackburn, J.E. Bradshaw, Potato Res. 58, 87 (2007)

    Article  Google Scholar 

  24. M. Rahman, D. Hussain, K.A. Malik, Y. Zafar, Plant Path. 54, 764 (2005)

    Article  CAS  Google Scholar 

  25. M. Padidam, S. Sawyer, C.M. Fauquet, Virology 265, 218 (1999)

    Article  PubMed  CAS  Google Scholar 

  26. R.W. Briddon, Mol. Plant Pathol. 4, 427 (2003)

    Article  PubMed  CAS  Google Scholar 

  27. J.M. Anderson, D.L. Bucholtz, A.E. Greene, M.G. Francki, S.M. Gray, H. Sharma, H.W. Ohm, K.L. Perry, Phytopathology 88, 851 (1998)

    Article  PubMed  CAS  Google Scholar 

  28. M. Prins, M. Laimer, E. Noris, J. Schubert, M. Wassenger, M. Tepfer, Mol. Plant Pathol. 9, 73 (2008)

    PubMed  CAS  Google Scholar 

  29. F.J. Perlak, R.W. Deaton, T.A. Armstrong, R.L. Fuchs, S.R. Sims, J.T. Greenplate, D.A. Fischhoff, Bio/Technology 8, 939 (1990)

    Article  PubMed  CAS  Google Scholar 

  30. J.C. Sanford, S.A. Johnston, J. Theor. Biol. 113, 395 (1985)

    Article  Google Scholar 

  31. N. Taylor, P. Chavariaga, K. Raemakers, D. Siritunga, P. Zhang, Plant Mol. Biol. 56, 671 (2004)

    Article  PubMed  CAS  Google Scholar 

  32. C. Desbeiz, H. Lecoq, Plant Pathol. 46, 809 (1997)

    Article  Google Scholar 

  33. J. Stanley, Vet. Microbiol. 88, 121 (2004)

    Article  Google Scholar 

  34. A. Lucioli, E. Noris, A. Brunetti, R. Tavazza, V. Ruzza, A.G. Castillo, E.R. Bejarano, G.P. Accoto, M. Tavaza, J. Virol. 77, 6785 (2003)

    Article  PubMed  CAS  Google Scholar 

  35. P. Hanson, S.K. Green, G. Kuo, Tomato Genet. Coop. Rep. 56, 17 (2006)

    Google Scholar 

  36. A. Brunetti, M. Tavazza, E. Noris, R. Tavazza, P. Caciagli, G. Ancora, S. Crespi, G.P. Acotto, Mol. Plant Microbe Interact. 10, 571 (1997)

    Article  CAS  Google Scholar 

  37. R. Goldbach, E. Bucher, M. Prins, Virus Res. 92, 207 (2003)

    Article  PubMed  CAS  Google Scholar 

  38. F. Heyraud, V. Matzeit, M. Kammann, S. Schaefer, J. Schell, B. Gronenborn, EMBO J. 12, 4445 (1993)

    PubMed  CAS  Google Scholar 

  39. A.I. Sanz, A. Fraile, F.G. Arnel, X. Zhou, D.J. Robinson, S. Khalid, T. Butt, B.D. Harrison, J. Gen. Virol. 81, 1839 (2000)

    PubMed  CAS  Google Scholar 

Download references

Acknowledgments

We would like to thank Miss. Shahnaz Akhtar, Mrs. Kalsoom Akhtar, Mr Saeed Faisal, and Mr. Karim Bukhsh for their help in tissue culture and glasshouse trials, respectively. We also acknowledge PSF and HEC for providing financial support to carry out this work. We also extend our thanks to Dr. John P. Carr, Department of Plant Sciences, University of Cambridge for providing access to his Lab and to carry out some hybridization work.

Author information

Authors and Affiliations

  1. Agricultural Biotechnology Division, National Institute for Biotechnology and Genetic Engineering (NIBGE), P. O. Box 577, Jhang Road, Faisalabad, Pakistan

    Jamil A. Hashmi, Yusuf Zafar, Muhammad Arshad, Shahid Mansoor & Shaheen Asad

Authors
  1. Jamil A. Hashmi
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Yusuf Zafar
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Muhammad Arshad
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Shahid Mansoor
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Shaheen Asad
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding authors

Correspondence to Yusuf Zafar or Shaheen Asad.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Hashmi, J.A., Zafar, Y., Arshad, M. et al. Engineering cotton (Gossypium hirsutum L.) for resistance to cotton leaf curl disease using viral truncated AC1 DNA sequences. Virus Genes 42, 286–296 (2011). https://doi.org/10.1007/s11262-011-0569-9

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11262-011-0569-9

Keywords

Search

Navigation