Abstract
A total of 135 unrelated sheep from nine Turkish native sheep breeds (Daglıc, Kivircik, Imroz, Chios, Morkaraman, Ivesi, Hemsin, Karayaka and Akkaraman) were investigated to determinate the maternal genetic diversity using a sequence of a 531-bp segment of the mtDNA control region. Analysis of the mtDNA control region sequence revealed 63 haplotypes and 53 polymorphic sites. Haplotype diversity, nucleotide diversity and the average number of nucleotide differences were estimated to be 0.9496 ± 0.011, 0.01407 ± 0.00060 and 7.456, respectively. The sequence analysis also revealed high level of genetic diversity among the native Turkish breeds. These breeds were grouped into three major maternal haplogroups: A, B and C, with one animal belonging from the Akkaraman breed to the rare haplogroup E. Irregular shape of mismatch distribution of haplogroup C could be an indicator that haplogroup C may represent different haplogroups. Contrarily to previous studies carried out on Turkish native breeds, majority of animals grouped in haplogroup A in the present study. This result and the irregular shape of mismatch curve of haplogroup C indicate that genetic structure of Turkish native sheep breeds could be more complicated than it is thought.
Similar content being viewed by others
References
Acar, H., 2010. Bioinformatic analysis in microsatellite-based genetic diversity of Turkish sheep breeds, (unpublished Msc thesis, Middle East Technical University).
Bruford, M.W., Brandley, D.G. and Luikart G., 2003. DNA markers reveal the complexity of livestock domestication, Nature, 4, 900–910.
Chen, S.Y., Duan, Z.Y., Xiangyu, T., Wu, S.F. and Zhang, Y.P., 2006. Origin, genetic diversity, and population structure of Chinese domestic sheep, Gene, 376(2), 216–223.
Demirci, S., Koban, E., Doğan, A. and Togan I., 2010. A Study on Mitochondrial DNA Haplogroups and control Region Sequences of Turkish Native Sheep Breeds International. In: Symposium on Health Informatics and Bioinformatics,(HIBIT’10) Antalya – Türkiye, 54.
Excoffier, L., Laval, G. and Schneider, S., 2005. Arlequin (version 3.0): An integrated software package for population genetics data analysis, Evolutionary Bioinformatics Online, 1, 47–50.
Guo, J., Du, L.X., Ma, Y.H., Guan, W.J., Li, H.B., Zhao, H.B. and Rao, S.Q., 2005. A novel maternal lineage revealed in sheep (Ovis aries), Animal Genetics, 36, 331–336.
Hiendleder, S., Mainz, K., Plante, Y. and Lewalski H., 1998. Analysis of mitochondrial DNA indicates that domestic sheep are derived from two different ancestral maternal sources: no evidence for contributions from Urial and Argali sheep, Journal of Heredity, 89, 113–120.
Hiendleder, S., Kaupe, B., Wassmuth, R. and Janke, A., 2002. Molecular analysis of wild and domestic sheep questions current nomenclature and provides evidence for domestication from two different subspecies, Proceedings of the Royal Society B: Biological Sciences, 269, 893–904.
Koban, E., 2004. Genetic Diversity of Native and Crossbreed Sheep Breeds in Anatolia, unpublished PhD thesis, Middle East Technical University)
Meadows, J.R.S., Li, K., Kantanen, J., Tapio, M., Sipos, W., Pardeshi, V., Gupta, V., Calvo, J.H., Whan, V., Norris B. and Kijas, J.W., 2005. Mitochodrial Sequence Reveals high Levels of Gene Flow Between Breeds of Domestic Sheep from Asia and Europe, Journal of Heredity, 96, 494–501.
Meadows, J.R.S., Cemal, I., Karaca, O., Gootwine, E. and Kijas, J.W., 2007. Five Ovine mitochondrial lineages identified from sheep breeds of the Near East, Genetics, 175, 1371-1379.
Meadows, J.R., Hiendleder, S. and Kijas, J.W., 2011. Haplogroup relationships between domestic and wild sheep resolved using a mitogenome panel, Heredity, 106(4), 700–706.
Pedrosa S., Uzun M., Arranz J.J., Gutiérrez-Gil B.F., San Primitivo F., Bayón Y., 2005. Evidence of three maternal lineages in Near Eastern sheep supporting multiple domestication events, Proceedings of the Royal Society B: Biological Sciences, 272, 2211–2217.
Pereira F., Davis, S.J.M., Pereira, L., McEvoy, B., Bradley, D.G. and Amorim, A., 2006. Genetic signatures of a Mediterranean influence in Iberian Peninsula sheep husbandry, Molecular Biology and Evolution, 23, 1420–1426.
Rozas, J., Sanchez-Delbarrio, J.C., Messeguer, J.C. and Rozas, J.C., 2003, DnaSP, DNA polymorphism analyses by the coalescent and other methods, Bioinformatics, 19, 2496–2497.
Tamura, K., Dudley, J., Nei, M. and Kumar, S., 2007. Epub 2007 MEGA4: Molecular Evolutionary Genetics Analysis (MEGA) software version 4.0, 24(8), 1596–1599
Tapio, M., Marzanov, N., Ozerov, M., Ćinkulov, M., Gonzarenko, G., Kiselyova, T., Murawski, M., Viinalass, H. and Kantanen, J., 2006. Sheep mitochondrial DNA variation in European, Caucasian and Central Asian areas, Molecular Biology and Evolution, 23, 1776–1783.
Uerpmann, H.P., 1996. Animal Domestication-Accident or Intention? The Origins and Spread of Agriculture and Pastoralism in Eurasia. (ed. Harris David, R.), UCL Press Ltd, London, pp. 227–237.
Vigne, J.D., Buitenhuis, H. and Davis, S., 1999, Les premiers pas de la domestication animale à l’Ouest de l’Euphrate : Chypre et l’Anatolie central, Paleorient, 25, 49–62.
Wood, N.J. and Phua, S.H., 1996. Variation in the control region sequence of the sheep mitochondrial genome, Animal Genetics, 27, 25–33.
Yüncü, E, Koban, E. and Togan, I., 2008. Mitochondrial DNA (mtDNA) haplogroup compositions of three native Turkish Sheep Breeds and their implications on the conservation studies. In: 31st International Society of Animal Genetics, Netherlands, 20–24 July. Poster No: 5014
Zeder M.A., 2008, Domestication and early agriculture in the Mediterranean Basin: Origins, diffusion, and impact, Proceedings of the National Academy of Sciences of the United States of America, 105:(33), 11597–11604.
Acknowledgments
This study was financially supported by the Scientific Research Project Council of Uludag University (project number, Z-2009/29). This manuscript was edited by the American Journal Experts (AJE).
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Oner, Y., Calvo, J.H. & Elmaci, C. Investigation of the genetic diversity among native Turkish sheep breeds using mtDNA polymorphisms. Trop Anim Health Prod 45, 947–951 (2013). https://doi.org/10.1007/s11250-012-0313-z
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s11250-012-0313-z