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Equipment used for testing anaerobic biodegradability and activity

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Abstract

Recently there has been a growing demand for information on the biodegradability and microbial toxicity of xenobiotic compounds. The environmental fate and effect of these chemicals in aerobic and anaerobic conditions has been extensively studied using a variety of different methods. This paper reviews the different protocols, equipment and instruments used in anaerobic biodegradation and activity tests, including the more sophisticated automated techniques recently developed. The different systems for detecting biogas production are given particular attention. As well as gasometric methods, the different instrumental techniques used to follow the fate of compounds under anaerobic conditions, via analysis of substrate consumption and product formation are evaluated.

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References

  • NR Adrian T Chow (2001) ArticleTitleIdentification of hydroxylamino-dinitroso-1,3,5-triazine as a transient intermediate formed during the anaerobic biodegradation of hexahydro-1,3,5-trinitro-1,3,5-triazine Environ. Toxicol. Chem. 20 IssueID9 1874–1877

    Google Scholar 

  • I Angelidaki JE Schmidt L Ellegaard BK Ahring (1998) ArticleTitleAn automatic system for simultaneous monitoring of gas evolution in multiple closed vessels J. Microbiol. Meth. 33 IssueID1 93–100

    Google Scholar 

  • NS Battersby V Wilson (1988) ArticleTitleEvaluation of a serum bottle technique for assessing the anaerobic biodegradability of organic chemicals under methanogenic conditions Chemosphere 17 IssueID12 2441–2460

    Google Scholar 

  • A Beaubien C Jolicoeur JF Alary (1988) ArticleTitleAutomated high sensitivity gas metering system for biological processes Biotechnol. Bioeng. 32 105–109

    Google Scholar 

  • RR Birch C Biver R Campagna WE Gledhill U Pagg J Steber H Reust WJ Bontinck (1989) ArticleTitleScreening of chemicals for anaerobic biodegradability Chemosphere 19 IssueID10–11 1527–1550

    Google Scholar 

  • AM Buswell HF Mueller (1952) ArticleTitleMechanism of fermentation Ind. Eng. Chem. 44 2441–2460

    Google Scholar 

  • A Cohen (1992) ArticleTitleEffects of some industrial-chemicals on anaerobic activity measured by Sequential Automated Methanometry (SAM) Water Sci. Technol. 25 IssueID7 11–20

    Google Scholar 

  • Concannon F, Reynolds PJ, Henningan A & Colleran E (1988) Development of a computerised continuous assay for specific methanogenic activity measurement. In: Proceedings of the 5th International Symposium on Anaerobic Digestion: Poster papers, A. Tilche and A. Rozzi (Eds), Monduzzi Editore, Bologna, Italy. pp. 177–181.

  • Council Directive (76/464)/EEC of May 1976 on pollution caused by certain dangerous substances discharged into aquatic environment of the Community. Official Journal of the European Communities L 129 pp. 0023–0029

  • Council Directive 96/61/EC of 24 September 1996 concerning integrated pollution prevention and control. Official Journal of the European Communities L 257, 10/10/1996 pp. 0026–0040

  • MJ Delafontaine J Hutschemakers HP Naveau EJ Nyns (1982) ArticleTitleImproved method for quantitative evaluation of the potential for methane production of mixed liquors through the determination of the content of co-enzyme F420 Biomass 2 115–125

    Google Scholar 

  • Directive (2000/60/EC) of the European Parliament and of the Council of 23 October 2000 establishing a framework for Community action in the field of water policy. Official Journal of the European Communities L 327, pp. 001–0072

  • J Dolfing WGBM Bloeman (1985) ArticleTitleActivity measurements as a tool to characterise the microbial composition of methanogenic environments J. Microbiol. Methods 4 1–12

    Google Scholar 

  • M Dupla T Conte JC Bouvier N Bernet JP Steyer (2004) ArticleTitleDynamic evaluation of a fixed bed anaerobic digestion process in response to organic overloads and toxicant shockloads Water Sci. Technol. 49 IssueID1 61–68

    Google Scholar 

  • ECETOC, (1988) European Chemical Industry Ecology and Toxicology Centre: Evaluation of anaerobic biodegradation. Technical Report No28, Brussels.

  • MT Garcia E Campos J Sanchez-Leal I Riboa (2000) ArticleTitleAnaerobic degradation and toxicity of commercial cationic surfactants in anaerobic screening tests Chemosphere 41 705–710

    Google Scholar 

  • MT Garcia E Campos J Sanchez-Leal I Riboa (1999) ArticleTitleEffect of alkyl chain length on the anaerobic biodegradability and toxicity of quaternary ammonium based surfactants Chemosphere 38 IssueID15 705–710

    Google Scholar 

  • M Glauser B Jenni M Aragno (1984) ArticleTitleAn inexpensive, automatic gas meter for laboratory-scale methane digesters and other gas-evolving systems J. Microbiol. Methods 2 159–164

    Google Scholar 

  • WE Gledhill (1979) Proposed standard practice for determination of the anaerobic biodegradability organic chemicals. Working Document draft 2 No. 35–24 American Society for Testing Materials Philadelphia

    Google Scholar 

  • LGM Gorris TM Kok Particlede BM Kroon C Drift Particlevan der GD Vogels (1988) ArticleTitleRelationship between methanogenic co-factor content and maximum specific methanogenic activity of anaerobic granular sludges Applied. Environ. Microbiol. 54 1126–1130

    Google Scholar 

  • AJ Guwy DL Hawkes FR Hawkes (1995) ArticleTitleOn-line low flow high-precision gas metering systems Water Research, 29 IssueID3 977–979

    Google Scholar 

  • W Gujer AJB Zehnder (1983) ArticleTitleConversion processes in anaerobic digestion Water. Sci. Technol. 15 127–167

    Google Scholar 

  • AJ Guwy FR Hawkes DL Hawkes AG Rozzi (1997) ArticleTitleHydrogen Production In a High Rate Fluidised Bed Anaerobic Digester Water Res. 31 IssueID6 1291–1298

    Google Scholar 

  • P Gwatkin D Drewill-Smith AG Lane (1986) ArticleTitleAn inexpensive electronic gas flow meter for use with small anaerobic digesters Environ. Technol. Lett. 7 565–570

    Google Scholar 

  • J Healy LY Young (1979) ArticleTitleAnaerobic biodegradation of eleven aromatic compounds to methane Applied Environ. Microbiol. 38 84–89

    Google Scholar 

  • HMSO (1988) Methods for the examination of waters and associated materials: The Assessment of Biodegradability in Anaerobic Sludges, pp. 1–19

  • M Huber U Meyer P Rys (2000) ArticleTitleBiodegradation mechanisms of linear alcohol ethoxylates under anaerobic conditions Environ. Sci. Technol. 34 IssueID9 1737–1741

    Google Scholar 

  • O Ince GK Anderson B Kasapgil (1995) ArticleTitleControl of organic loading rate using the specific methanogenic activity test during start-up of an anaerobic-digestion system Water Res. 29 IssueID1 349–355

    Google Scholar 

  • ISO 11734 International Standard (First Edition 15-12-1995) Water quality-evaluation of the “ultimate” anaerobic biodegradability of organic compounds in digested sludge-Method by measurement of the biogas production

  • ISO/DIS 14853 (1999) Determination of the ultimate anaerobic biodegradability of plastic materials in an aqueous system-Method by measurement of biogas production

  • A James CAL Chernicharo CMM Campos (1990) ArticleTitleThe development of a new methodology for the assessment of specific methanogenic activity Water Res. 24 IssueID7 813–825

    Google Scholar 

  • LD Johnson JC Young (1983) Inhibition of anerobic digestion by organic priority pollutants 1441 Journal Water Pollution Control Federation 55 (12)

    Google Scholar 

  • Jörg R. (2000) Anaerobe Abbaubarkeit von Kunststoffen in aquatischen. Screening-Tests mit dem Methanomat, Stutt. Ber. Abfall. Wirtsch., Bd. 76, Bielefeld: E. Schmidt.

  • IJ Kugelman PL McCarty (1965) ArticleTitleCation toxicity and stimulation in waste treatment J. Water Control Federation 37 97

    Google Scholar 

  • Kuss ML & Young JC (1992) Method and apparatus for measuring gas flow using bubble volume. U.S. Patent No. 5,092,181 (March 1992)

  • PL McCarty (1964) Anaerobic Waste Treatment Fundamentals Part 1 Public Works New York 107

    Google Scholar 

  • TL Miller MJ Wolin (1974) ArticleTitleA serum bottle modification of the Hungate techniqu for cultivating obligate anaerobes Applied Microbiology. 27 985–987

    Google Scholar 

  • R Moletta G Albagnac (1982) ArticleTitleA gas meter for low rates of flow: application to the methane fermentation Biotechnol. Lett. 4 IssueID5 319–322

    Google Scholar 

  • FE Mosey XA Fernandez (1989) ArticleTitlePatterns of hydrogen in biogas from the anaerobic digestion of milk-sugars Water. Sci. Technol. 21 4/5 187–196

    Google Scholar 

  • M Mota P Besle P Strehaiano G Goma (1987) ArticleTitleA simple device for fed-batch control in alcoholic fermentation Biotechnol. Bioeng. 29 775–777

    Google Scholar 

  • V O’Flaherty P Lens B Leahy E Colleran (1988) ArticleTitleLong-term competition between sulphate-reducing and methane-producing bacteria during full-scale anaerobic treatment of citric acid production wastewater Water Res. 32 IssueID3 815–825

    Google Scholar 

  • WF Owen DC Stuckey JB Healy LY Young PL McCarty (1979) ArticleTitleBioassay for monitoring biochemical methane potential and anaerobic toxicity Water Res. 13 485–492

    Google Scholar 

  • GC Premier R Dinsdale AJ Guwy FR Hawkes DL Hawkes SJ Wilcox (1997) ArticleTitleSimple Black Box Models Predicting Potential Control Parameters During Disturbances To a Fluidised Bed Anaerobic Reactor Water Sci. Technol. 36 IssueID6–7 229–237

    Google Scholar 

  • A Rozzi L Castellazzi RE Speece (2002) ArticleTitleAcetoclastic methanogenic activity measurement by a titration bioassay Biotechnol. Bioeng. 77 IssueID1 20–26

    Google Scholar 

  • A Rozzi E Remigi C Buckley (2000) ArticleTitleMethanogenic activity measurements by the MAIA biosensor: instructions guide Water Sci. Technol. 44 IssueID4 287–294

    Google Scholar 

  • DR Shelton JM Tiedje (1984) ArticleTitleGeneral method for determining anaerobic biodegradation potential Applied and Environ. Microbiol. 47 IssueID4 850–857

    Google Scholar 

  • V Stergar JZ Koncan (2002) ArticleTitleThe determination of anaerobic biodegradability of pharmaceutical waste using advanced bioassay technique Chem. Biochem. Eng. Quart. 16 IssueID1 17–24

    Google Scholar 

  • JP Steyer JC Bouvier T Conte P Gras J Harmand JP Delgenes (2002) ArticleTitleOn-line measurements of COD, TOC, VFA, total and partial alkalinity in anaerobic digestion processes using infra-red spectrometry Water Sci. Technol. 45 IssueID10 133–138

    Google Scholar 

  • WW Umbreit RH Burris JF Stayffer (1964) Manometric Techniques Burgess Berks

    Google Scholar 

  • MC Veiga M Soto R Mendez JM Lema (1990) ArticleTitleA new device for measurement and control of gas production by bench scale anaerobic digesters Water Res. 24 IssueID12 1551–1554

    Google Scholar 

  • D Valke W Verstraete (1983) ArticleTitleA practical method to estimate the aceoclastic methanogenic biomass in anaerobic sludges J. Water Pollution Control Federation 55 1191–1195

    Google Scholar 

  • L Berg ParticleVan der C Lentz R Athey E Rooke (1974) ArticleTitleAssessment of methanogenic activity in anaerobic digestion: Apparatus and method Biotechnol. Bioeng. 16 1459–1469

    Google Scholar 

  • Young JC, Kuss ML & Nelson MA (1991) Use of anaerobic respirometers for measuring gas production in toxicity and treatability tests. 84th Annual Meeting of the Air and Waste Management Association Vancouver, B.C. June 16–21.

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  1. Wastewater Treatment Unit, School of Applied Sciences, University of Glamorgan, Pontypridd, Mid Glamorgan, CF37 1DL, UK

    A. J. Guwy

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  1. A. J. Guwy
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Guwy, A.J. Equipment used for testing anaerobic biodegradability and activity. Rev Environ Sci Biotechnol 3, 131–139 (2004). https://doi.org/10.1007/s11157-004-1290-0

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