References
Kozera B, Rapacz M (2013) Reference genes in real-time PCR. J Appl Genet 54:391–406
Bustin SA, Benes V, Garson JA, Hellemans J, Huggett J, Kubista M et al (2009) The MIQE guidelines: minimum information for publication of quantitative real-time PCR experiments. Clin Chem 55:611–622
Van Rijn SJ, Riemers FM, van den Heuvel D, Wolfswinkel J, Hofland L, Meij BP et al (2014) Expression stability of reference genes for quantitative RT-PCR of healthy and diseased pituitary tissue samples varies between humans, mice, and dogs. Mol Neurobiol 49:893–899
Acknowledgments
We thank Prof. Wiesław Bonicki and Jacek Kunicki from the Department of Neurosurgery at the Maria Sklodowska-Curie Memorial Cancer Center and Institute of Oncology in Warsaw, Poland for their work in collecting tissue samples and clinical data.
Conflict of interest
The authors declare that they have no conflict of interest.
Ethical standard
All procedures performed in studies involving human participants were in accordance with the ethical standards of the institutional and/or national research committee and with the 1964 Helsinki declaration and its later amendments or comparable ethical standards. Informed consent was obtained from all individual participants included in the study.
Author information
Authors and Affiliations
Corresponding author
Electronic supplementary material
Below is the link to the electronic supplementary material.
Rights and permissions
About this article
Cite this article
Bujko, M., Rusetska, N. & Mikula, M. Validating candidate reference genes for qRT-PCR-based gene expression analysis in nonfunctioning pituitary adenomas. Pituitary 19, 110–112 (2016). https://doi.org/10.1007/s11102-015-0656-3
Published:
Issue Date:
DOI: https://doi.org/10.1007/s11102-015-0656-3