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The Effect of n-acetylcysteine and Deferoxamine on Exercise-induced Oxidative Damage in Striatum and Hippocampus of Mice

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Abstract

The aim of this study was to analyze the effects of intense exercise on brain redox status, associated with antioxidant supplementation of N-acetylcysteine (NAC), deferoxamine (DFX) or a combination of both. Seventy-two C57BL-6 adult male mice were randomly assigned to 8 groups: control, NAC, DFX, NAC plus DFX, exercise, exercise with NAC, exercise with DFX, and exercise with NAC plus DFX. They were given antioxidant supplementation, exercise training on a treadmill for 12 weeks, and sacrificed 48 h after the last exercise session. Training significantly increased (P < 0.05) soleus citrate synthase (CS) activity when compared to control. Blood lactate levels classified the exercise as intense. Exercise significantly increased (P < 0.05) oxidation of biomolecules and superoxide dismutase activity in striatum and hippocampus. Training significantly increased (P < 0.05) catalase activity in striatum. NAC and DFX supplementation significantly protected (P < 0.05) against oxidative damage. These results indicate intense exercise as oxidant and NAC and DFX as antioxidant to the hippocampus and the striatum.

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Acknowledgments

This research was supported by grants from CNPq/MCT (Brazil), CAPES/MEC (Brazil), and UNESC (Brazil).

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Authors and Affiliations

  1. Laboratory of Exercise Physiology, PPGCS, Universidade do Extremo Sul Catarinense, Criciuma, SC, 88806-000, Brazil

    Aderbal S. Aguiar Jr, Talita Tuon, Fernanda S. Soares, Luís Gustavo C. da Rocha, Paulo César Silveira & Ricardo A. Pinho

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  1. Aderbal S. Aguiar Jr
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  2. Talita Tuon
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  3. Fernanda S. Soares
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  4. Luís Gustavo C. da Rocha
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  5. Paulo César Silveira
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  6. Ricardo A. Pinho
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Correspondence to Ricardo A. Pinho.

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Aguiar, A.S., Tuon, T., Soares, F.S. et al. The Effect of n-acetylcysteine and Deferoxamine on Exercise-induced Oxidative Damage in Striatum and Hippocampus of Mice. Neurochem Res 33, 729–736 (2008). https://doi.org/10.1007/s11064-007-9485-8

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  • DOI: https://doi.org/10.1007/s11064-007-9485-8

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