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Pseudocercospora griseola Causing Angular Leaf Spot on Phaseolus vulgaris Produces 1,8-Dihydroxynaphthalene-Melanin

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Abstract

Pseudocercospora griseola is the causal agent of angular leaf spot of common bean (ALS). It has undergone parallel coevolution with its host and two major groups have been defined, “Andean” (P. griseola f. griseola) and “Mesoamerican” (P. griseola f. mesoamericana). The aim of this study was to analyze the nature and the level of the dark pigment synthesized by the representatives of each group. After 21 days of incubation on potato dextrose agar medium, P. griseola f. griseola isolate S3b developed colonies with diameters of 17.5 ± 1.3 mm and concentric rings of pigmentation. Isolate T4 of P. griseola f. mesoamericana presented smaller colonies (9.9 ± 0.3 mm) with a uniform dark-gray color. Both isolates, S3b and T4, produced the same pigment, a 1,8-dihydroxynaphthalene-melanin, although different in quantity and structural features as suggested by the IR spectrum. The P. griseola f. griseola isolate S3b had a higher growth rate and melanin content as well as smaller sensitivity to melanin synthesis inhibitors compared to the isolate T4 of P. griseola f. mesoamericana. These results suggest a possible link between melanin and growth in P. griseola.

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Acknowledgments

Saparrat, M. C. N. and Stenglein, S. A. are researchers from the Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Argentina. Balatti, P. A. is a researcher from the Comisión de Investigaciones Científicas de la Provincia de Buenos Aires (CICBA), Argentina. This research was partially supported by grants from CONICET, CICBA, UNLP (A173 Project), Argentina.

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Authors and Affiliations

  1. Instituto de Fisiología Vegetal (INFIVE), Universidad Nacional de La Plata (UNLP)-CCT-La Plata-Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Diag. 113 y 61, CC 327, 1900, La Plata, Argentina

    Mario C. N. Saparrat, Geraldine E. Fermoselle & Pedro A. Balatti

  2. Instituto de Botánica Spegazzini, Facultad de Ciencias Naturales y Museo, Universidad Nacional de La Plata, 53 # 477, 1900, La Plata, Argentina

    Mario C. N. Saparrat

  3. Cátedra de Microbiología Agrícola, Facultad de Ciencias Agrarias y Forestales, Universidad Nacional de La Plata, 60 y 119, 1900, La Plata, Argentina

    Mario C. N. Saparrat, Geraldine E. Fermoselle & Pedro A. Balatti

  4. Laboratorio de Biología Funcional y Biotecnología (BIOLAB)-CEBB-CONICET, Facultad de Agronomía de Azul, UNCPBA, Rep. de Italia 780, 7300, Azul, Buenos Aires, Argentina

    Sebastián A. Stenglein

  5. Instituto Fitotécnico de Santa Catalina, Facultad de Ciencias Agrarias y Forestales, Universidad Nacional de La Plata, 1842, Buenos Aires, Argentina

    Mónica B. Aulicino

  6. Cátedra de Fitopatología-CIDEFI, Facultad de Ciencias Agrarias y Forestales, Universidad Nacional de La Plata, 60 y 119, CC31, 1900, La Plata, Argentina

    Pedro A. Balatti

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  1. Mario C. N. Saparrat
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  2. Geraldine E. Fermoselle
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  3. Sebastián A. Stenglein
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  4. Mónica B. Aulicino
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  5. Pedro A. Balatti
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Correspondence to Mario C. N. Saparrat.

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Saparrat, M.C.N., Fermoselle, G.E., Stenglein, S.A. et al. Pseudocercospora griseola Causing Angular Leaf Spot on Phaseolus vulgaris Produces 1,8-Dihydroxynaphthalene-Melanin. Mycopathologia 168, 41–47 (2009). https://doi.org/10.1007/s11046-009-9194-8

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