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Serum microRNAs; miR-30c-5p, miR-223-3p, miR-302c-3p and miR-17-5p could be used as novel non-invasive biomarkers for HCV-positive cirrhosis and hepatocellular carcinoma

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Abstract

Recently, serum miRNAs have been evolved as possible biomarkers for different diseases including hepatocellular carcinoma and other types of cancers. Investigating certain serum miRNAs as novel non-invasive markers for early detection of HCV-positive cirrhosis and hepatocellular carcinoma (HCC). The expression profiles of 58 miRNA were analyzed in patient’s plasma of chronic hepatitis C (CHC), HCV-positive cirrhosis and HCV-positive HCC and compared with control group samples. Totally 94 plasma samples; 64 patient plasma (26 CHC, 30 HCV-positive cirrhosis, 8 HCV-positive HCC) and 28 control group plasma, were included. The expression profiles of 58 miRNAs were detected for all patient and control group plasma samples by qRT-PCR using BioMarkTM 96.96 Dynamic Array (Fluidigm Corporation) system. In CHC group, expression profiles of miR-30a-5p, miR-30c-5p, miR-206 and miR-302c-3p were found significantly deregulated (p < 0.05) when compared versus control group. In HCV-positive cirrhosis group, expression profiles of miR-30c-5p, miR-223-3p, miR-302c-3p, miR-17-5p, miR-130a-3p, miR-93-5p, miR-302c-5p and miR-223-3p were found significantly deregulated (p < 0.05). In HCV-positive HCC group, expression profiles of miR-17-5p, miR-223-3p and miR-24-3p were found significant (p < 0.05). When all groups were compared versus control, miR-30c-5p, miR-223-3p, miR-302c-3p and miR-17-5p were found significantly deregulated for cirrhosis and HCC. These results imply that miR-30c-5p, miR-223-3p, miR-302c-3p and miR-17-5p could be used as novel non-invasive biomarkers of HCV-positive HCC in very early, even at cirrhosis stage of liver disease.

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Acknowledgments

All authors thank to Mersin University Scientific Research Fund for their financial support [Project no. BAP-SBE FM (ZÖ) 2011-5 YL].

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Authors and Affiliations

  1. Department of Pharmaceutical Microbiology, Faculty of Pharmacy, Mersin University, Yenisehir, Mersin, 33169, Turkey

    Zehra Oksuz, Mehmet Sami Serin & Aylin Dogen

  2. Advanced Technology, Research, and Education Centre, Mersin University, Mersin, Turkey

    Mehmet Sami Serin & Engin Kaplan

  3. Department of Medical Microbiology, Faculty of Medicine, Mersin University, Mersin, Turkey

    Seda Tezcan, Gonul Aslan & Gurol Emekdas

  4. Department of Gastroenterology, Faculty of Medicine, Mersin University, Mersin, Turkey

    Orhan Sezgin & Engin Altintas

  5. Department of Hematology, Faculty of Medicine, Mersin University, Mersin, Turkey

    Eyup Naci Tiftik

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  1. Zehra Oksuz
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  2. Mehmet Sami Serin
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  3. Engin Kaplan
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Correspondence to Mehmet Sami Serin.

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Oksuz, Z., Serin, M.S., Kaplan, E. et al. Serum microRNAs; miR-30c-5p, miR-223-3p, miR-302c-3p and miR-17-5p could be used as novel non-invasive biomarkers for HCV-positive cirrhosis and hepatocellular carcinoma. Mol Biol Rep 42, 713–720 (2015). https://doi.org/10.1007/s11033-014-3819-9

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  • DOI: https://doi.org/10.1007/s11033-014-3819-9

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