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Phenotypic profiling and gene expression analyses for aromatic and volatile compounds in Chamoes (Cucumis melo)

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Abstract

Gotgam chamoe (GgC), a native oriental melon in Korea, is known to possess the aroma of a dried persimmon, an agronomic relevance for melon breeding program. The volatile compounds and the transcript levels of aromatic compound genes in cultivar (Ohbokggul chamoe [OC]) and GgC were profiled. A total of 62 volatile compounds were identified and quantified. Twenty-eight volatile compounds were specific to either the OC or the GgC. The amounts of volatile alcohol, saturated hydrocarbon, and unsaturated hydrocarbon compounds were 2.2, 2.7, and 1.1 times higher in OC, respectively. The amounts of ketone volatiles were 1.2 times higher in GgC, whereas the total amounts of esters were similar. In the shikimate pathway, transcriptional patterns with the fruit parts were different between the two chamoes for CmDAHPS, CmDHD/SDH, and CmEPSPS. The expression levels of all six genes investigated, especially CmCS, were highest in the peel of both chamoes compared to the other parts. The transcript levels of the aromatic amino acid biosynthesis genes demonstrate that phenylalanine and tyrosine are present more in edible parts of the chamoe, while tryptophan may be accumulated low in the chamoe. In addition, phenylalanine and tryptophan are synthesized more in GgC than the OC.

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Abbreviations

Cm :

Cucumis melo

PE:

Peel

PU:

Pulp

S:

Stalk

OC:

Ohbokggul chamoe

GgC:

Gotgam chamoe

GC:

Gas chromatography

E4P:

Erythrose 4-phosphate

PEP:

Phosphoenolpyruvate

CM:

Chorismatemutase

AS:

Anthranilate synthase

DAHPS:

3-deoxy-d-arabino-heptulosonate 7-phosphate synthase

CS:

Chorismate synthase

EPSP:

5-enolpyruvylshikimate 3-phosphate synthase

AAT:

Alcohol acetyltransferase

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Acknowledgments

This work was financially supported by Grants from the Next-Generation Bio Green 21 Program (No. PJ008200), Cabbage Genomics assisted breeding supporting Center (CGC) research programs, and Hallym University Research Fund (HRF-201302-006) funded by Rural Development Administration and Ministry for Food, Agriculture, Forestry and Fisheries of the Korean Government, and Hallym University, respectively.

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Authors and Affiliations

  1. Plant Systems Engineering Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), 125 Gwahangno, Yuseong-gu, Daejeon, 305-806, Korea

    Jeongyeo Lee, Jungeun Kim, Sung Ran Min & HyeRan Kim

  2. Division of Environment-Friendly Research, Gyeongsangnam-do Agricultural Research and Extension Service, Jinju, 660-360, Korea

    Min Keun Kim

  3. Department of Food Science and Nutrition, Hallym University, 1 Hallymdaehak-gil, Chuncheon, 200-702, Gangwon-do, Korea

    Seung Hwan Hwang & Soon Sung Lim

  4. Breeding & Research Institute, Nongwoo Bio Co., LTD, Yeoju, Kyonggi-do, 469-885, Korea

    Jong Moon Ahn

  5. Department of Crop Science, College of Agriculture & Life Sciences, Chungnam National University, 79 Daehangno, Yuseong-gu, Daejeon, 305-764, Korea

    Sang Un Park

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  1. Jeongyeo Lee
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  2. Min Keun Kim
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  8. Soon Sung Lim
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Correspondence to Soon Sung Lim or HyeRan Kim.

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Jeongyeo Lee and Min Keun Kim have contributed equally to this work.

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Lee, J., Kim, M.K., Hwang, S.H. et al. Phenotypic profiling and gene expression analyses for aromatic and volatile compounds in Chamoes (Cucumis melo). Mol Biol Rep 41, 3487–3497 (2014). https://doi.org/10.1007/s11033-014-3211-9

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