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Differentially expressed proteins associated with myogenesis and adipogenesis in skeletal muscle and adipose tissue between bulls and steers

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Abstract

The objective of this study was to identify some proteins associated with testosterone-related differences in myogenesis and adipogenesis between bulls and steers. Global proteins were monitored in skeletal muscle and adipose tissue from bulls (n = 20) and steers (n = 20), respectively. We identified four differentially expressed (twofold or more) proteins in skeletal muscle from bulls, myosin light chain 1 (MLC1), ankyrin repeat domain-containing protein 1 (ANKRD1) and heat shock protein beta 1 (HSPB1) that were up-regulated and cofilin 2 (CFL2) that was down-regulated, and also identified two down-regulated proteins in adipose tissue, transaldolase 1 (TALDO1) and l-lactate dehydrogenase B chain (LDHB). In vitro, after myogenic differentiation of a bovine cell line, the mRNA expression of HSPB1 not only increased approximately tenfold in response to differentiation but threefold in response to testosterone addition, respectively, but that of ANKRD1 and CFL2 did not significantly change in response to myogenic differentiation or testosterone addition. Likewise, after adipogenic differentiation of a bovine cell line, the mRNA expression of TALDO1 and LDHB did not significantly vary in response to adipogenic differentiation or testosterone addition. Therefore, we suggest that HSPB1 could have an important role during testosterone-related myogenesis.

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Acknowledgments

This research was supported by a grant to M. Baik from the National Research Laboratory Program through the National Research Foundation of Korea (NRF), funded by the Ministry of Education, Science and Technology, Republic of Korea (ROA-2007-000-20057-0).

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Authors and Affiliations

  1. Department of Agricultural Biotechnology, Research Institute for Agriculture and Life Sciences, Seoul National University, Seoul, 151-921, Republic of Korea

    Qiankun Zhang, Jung-A Han, Sang Kee Kang, Nam Kyung Lee & Yun-Jaie Choi

  2. Department of Animal Science and PNU-Special Animal Biotechnology Center, Pusan National University, Miryang, Gyeongnam, 627-706, Republic of Korea

    Hong-Gu Lee

  3. Department of Molecular Biotechnology, WCU-RNNM, Biotechnology Research Institute, Chonnam National University, Gwangju, 500-757, Republic of Korea

    Myunggi Baik

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  1. Qiankun Zhang
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Correspondence to Yun-Jaie Choi.

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Qiankun Zhang and Hong-Gu Lee contributed equally to this study.

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Zhang, Q., Lee, HG., Han, JA. et al. Differentially expressed proteins associated with myogenesis and adipogenesis in skeletal muscle and adipose tissue between bulls and steers. Mol Biol Rep 39, 953–960 (2012). https://doi.org/10.1007/s11033-011-0821-3

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