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Identification and validation of powdery mildew (Podosphaera xanthii)-resistant loci in recombinant inbred lines of cucumber (Cucumis sativus L.)

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Abstract

Powdery mildew limits cucumber production worldwide. Most resistant cucumber cultivars become susceptible to powdery mildew at low temperatures. Resistance within a wide temperature range is therefore desirable for cucumber production. We constructed a cucumber genetic linkage map based on a population of 111 recombinant inbred lines derived from a cross between CS-PMR1, with strong and temperature-independent resistance, and Santou, with moderate and temperature-dependent resistance. The map spans 693.0 cM and consists of 296 markers segregating into seven linkage groups; the markers include 289 simple sequence repeats (SSRs), six sequence characterized amplified regions, and one inter simple sequence repeat. Due to the presence of 150 common SSR markers, we were able to compare our map with previously published maps obtained by using populations derived from inter- or intra-variety crosses. We also evaluated powdery mildew resistance of the recombinant inbred lines and identified seven quantitative trait loci (QTL) contributed by CS-PMR1 and two QTL contributed by Santou. Four QTL (pm3.1, pm5.1, pm5.2 and pm5.3) were successfully validated by using populations derived from residual heterozygous lines. Some of the QTL identified in our study are in good agreement with previously published results obtained with materials of different origin. The markers reported here would be useful for introducing high and temperature-independent resistance by accumulation of QTL from CS-PMR1 and Santou.

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Acknowledgments

We thank Dr. M. Yano for his valuable advice and Dr. S. Huang for kindly providing SSR marker information prior to publication. We are grateful to S. Negoro, T. Yamakawa, K. Takeuchi and S. Toyoda for their technical assistance. This work was supported by NARO Research Project No. 211 ‘Establishment of Integrated Basis for Development and Application of Advanced Tools for DNA Marker-Assisted Selection in Horticultural Crops’.

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  1. Y. Sakata

    Present address: NARO Kyushu Okinawa Agricultural Research Center, Kurume, Fukuoka, 839-8503, Japan

Authors and Affiliations

  1. NARO Institute of Vegetable and Tea Science, Tsu, Mie, 514-2392, Japan

    N. Fukino, Y. Yoshioka, M. Sugiyama, Y. Sakata & S. Matsumoto

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  1. N. Fukino
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  2. Y. Yoshioka
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Correspondence to N. Fukino.

Electronic supplementary material

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11032_2013_9867_MOESM1_ESM.pdf

ESM 1 Comparison of marker distribution and linkage group lengths in the maps from 3 studies: hs map (Ren et al. 2009)a, ss map (Miao et al. 2011)b and CS map (this study)c (PDF 49 kb)

11032_2013_9867_MOESM2_ESM.pdf

ESM 2 Comparison of marker order and map distance in the linkage maps constructed by using RILs derived from inter-varietal (center: hs map, Ren et al. 2009) and intra-varietal crosses (left: ss map, Miao et al. 2011; right: CS map, this study). Common markers are connected with lines. Ruler on the left indicates linkage group length (PDF 427 kb)

11032_2013_9867_MOESM3_ESM.pdf

ESM 3 Positions and LOD scores of QTL pm4.1 for powdery mildew resistance in 109 RILs derived from C. sativus CS-PMR1 × ‘Santou’. Horizontal lines represent LOD thresholds at the 0.05 level of significance. LD25 and LD20, leaf disc assays at 25 and 20 °C, respectively; GH-S and GH-A, evaluation of naturally occurring disease in late spring to summer and in autumn, respectively (PDF 353 kb)

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Fukino, N., Yoshioka, Y., Sugiyama, M. et al. Identification and validation of powdery mildew (Podosphaera xanthii)-resistant loci in recombinant inbred lines of cucumber (Cucumis sativus L.). Mol Breeding 32, 267–277 (2013). https://doi.org/10.1007/s11032-013-9867-3

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