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Solution NMR structure of Alr2454 from Nostoc sp. PCC 7120, the first structural representative of Pfam domain family PF11267

  • Published:
Journal of Structural and Functional Genomics

Abstract

Protein domain family PF11267 (DUF3067) is a family of proteins of unknown function found in both bacteria and eukaryotes. Here we present the solution NMR structure of the 102-residue Alr2454 protein from Nostoc sp. PCC 7120, which constitutes the first structural representative from this conserved protein domain family. The structure of Nostoc sp. Alr2454 adopts a novel protein fold.

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Abbreviations

DSS:

2,2-Dimethyl-2-silapentane-5-sulfonic acid

DTT:

Dithiothreitol

HSQC:

Heteronuclear single quantum coherence

IPTG:

Isopropyl β-D-1-thiogalactopyranoside

NOE:

Nuclear Overhauser effect

NESG:

Northeast Structural Genomics Consortium

PDB:

Protein Data Bank

RMSD:

Root-mean-square-deviation

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Acknowledgments

The authors thank Roberto Tejero, Barry Honig, Bomina Yu, Colleen Ciccosanti, and G.V.T. Swapna for helpful discussions. This research was supported by National Institute of General Medical Sciences Protein Structure Initiative (PSI-Biology) program grants U54-GM074958 and U54-GM094597.

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Authors and Affiliations

  1. Department of Molecular Biology and Biochemistry, Center for Advanced Biotechnology and Medicine, and the Northeast Structural Genomics Consortium, Rutgers, The State University of New Jersey, Piscataway, NJ, 08854, USA

    James M. Aramini, Dong Yup Lee, Haleema Janjua, Rong Xiao, Thomas B. Acton, John K. Everett & Gaetano T. Montelione

  2. Department of Biochemistry and Medical Biophysics, Center for Computational Biology and Bioinformatics, and the Northeast Structural Genomics Consortium, Columbia University, New York, NY, 10032, USA

    Donald Petrey

  3. Department of Biochemistry, Robert Wood Johnson Medical School, University of Medicine and Dentistry of New Jersey, Piscataway, NJ, 08854, USA

    Gaetano T. Montelione

Authors
  1. James M. Aramini
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  2. Donald Petrey
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  3. Dong Yup Lee
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Corresponding authors

Correspondence to James M. Aramini or Gaetano T. Montelione.

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Aramini, J.M., Petrey, D., Lee, D.Y. et al. Solution NMR structure of Alr2454 from Nostoc sp. PCC 7120, the first structural representative of Pfam domain family PF11267. J Struct Funct Genomics 13, 171–176 (2012). https://doi.org/10.1007/s10969-012-9135-5

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  • DOI: https://doi.org/10.1007/s10969-012-9135-5

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