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Purification and characterization of a lectin, BPL-3, from the marine green alga Bryopsis plumosa

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Abstract

A novel lectin was isolated and characterized from Bryopsis plumosa (Hudson) Agardh and named BPL-3. This lectin showed specificity to N-acetyl-d-galactosamine as well as N-acetyl-d-glucosamine and agglutinated human erythrocytes of all blood types, showing slight preference to the type A. SDS-PAGE and MALDI-TOF MS data showed that BPL-3 was a monomeric protein with molecular weight of 11.5 kDa. BPL-3 was a non-glycoprotein with pI value of ∼7.0. It was stable in high temperatures up to 70°C and exhibited optimum activity in pH 5.5–10. The N-terminal and internal amino acid sequences of the lectin were determined by Edman degradation and enzymatic digestion, which showed no sequence homology to any other reported proteins. The full sequence of the cDNA encoding this lectin was obtained from PCR using cDNA library, and the degenerate primers were designed from the N-terminal amino acid sequence. The size of the cDNA was 622 bp containing single ORF encoding the lectin precursor. This lectin showed the same sugar specificity to previously reported lectin, Bryohealin, involved in protoplast regeneration of B. plumosa. However, the amino acid sequences of the two lectins were completely different. The homology analysis of the full cDNA sequence of BPL-3 showed that it might belong to H lectin group, which was originally isolated from Roman snails.

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Acknowledgements

This work was supported by a grant (RP-2010-AQ-049) from the National Fisheries Research and Development Institute, Korea.

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Authors and Affiliations

  1. Department of Biology, Kongju National University, Kongju, Chungnam, 314-701, South Korea

    Jong Won Han, Kang Sup Yoon, Tatyana A. Klochkova & Gwang Hoon Kim

  2. Seaweed Research Center, National Fisheries Research and Development Institute, Mokpo, 530-131, South Korea

    Mi-Sook Hwang

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  1. Jong Won Han
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  2. Kang Sup Yoon
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  3. Tatyana A. Klochkova
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  4. Mi-Sook Hwang
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  5. Gwang Hoon Kim
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Correspondence to Gwang Hoon Kim.

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Han, J.W., Yoon, K.S., Klochkova, T.A. et al. Purification and characterization of a lectin, BPL-3, from the marine green alga Bryopsis plumosa . J Appl Phycol 23, 745–753 (2011). https://doi.org/10.1007/s10811-010-9575-x

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